Experiment: HBM-CucXylem
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pntB and pntA are separated by 10 nucleotides pntA and LU632_RS11440 are separated by 228 nucleotides
LU632_RS11430: pntB - Re/Si-specific NAD(P)(+) transhydrogenase subunit beta, at 2,180,527 to 2,181,915
pntB
LU632_RS11435: pntA - Re/Si-specific NAD(P)(+) transhydrogenase subunit alpha, at 2,181,926 to 2,183,458
pntA
LU632_RS11440: LU632_RS11440 - IS5 family transposase, at 2,183,687 to 2,184,454
_RS11440
Position (kb)
2181
2182
2183
2184 Strain fitness (log2 ratio)
-1
0
1
2 at 2181.114 kb on + strand, within pntB at 2181.142 kb on - strand, within pntB at 2181.142 kb on - strand, within pntB at 2181.142 kb on - strand, within pntB at 2181.143 kb on - strand, within pntB at 2181.147 kb on - strand, within pntB at 2181.147 kb on - strand, within pntB at 2181.147 kb on - strand, within pntB at 2181.147 kb on - strand, within pntB at 2181.253 kb on - strand, within pntB at 2181.328 kb on - strand, within pntB at 2181.387 kb on + strand, within pntB at 2181.388 kb on - strand, within pntB at 2181.427 kb on - strand, within pntB at 2181.600 kb on - strand, within pntB at 2181.887 kb on + strand at 2181.887 kb on + strand at 2181.887 kb on + strand at 2181.887 kb on + strand at 2181.887 kb on + strand at 2181.888 kb on - strand at 2181.898 kb on + strand at 2181.902 kb on - strand at 2182.140 kb on + strand, within pntA at 2182.141 kb on - strand, within pntA at 2182.141 kb on - strand, within pntA at 2182.151 kb on + strand, within pntA at 2182.169 kb on + strand, within pntA at 2182.442 kb on - strand, within pntA at 2182.515 kb on - strand, within pntA at 2182.713 kb on - strand, within pntA at 2183.099 kb on - strand, within pntA at 2183.157 kb on - strand, within pntA at 2183.189 kb on - strand, within pntA at 2183.189 kb on - strand, within pntA at 2183.254 kb on + strand, within pntA at 2183.271 kb on - strand, within pntA at 2183.311 kb on - strand at 2183.454 kb on - strand at 2183.458 kb on + strand at 2183.627 kb on - strand at 2183.695 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-CucXylem remove 2,181,114 + pntB LU632_RS11430 0.42 -0.2 2,181,142 - pntB LU632_RS11430 0.44 -0.8 2,181,142 - pntB LU632_RS11430 0.44 +1.2 2,181,142 - pntB LU632_RS11430 0.44 +1.2 2,181,143 - pntB LU632_RS11430 0.44 +0.8 2,181,147 - pntB LU632_RS11430 0.45 -0.3 2,181,147 - pntB LU632_RS11430 0.45 +1.2 2,181,147 - pntB LU632_RS11430 0.45 +0.1 2,181,147 - pntB LU632_RS11430 0.45 -0.9 2,181,253 - pntB LU632_RS11430 0.52 +1.8 2,181,328 - pntB LU632_RS11430 0.58 -0.3 2,181,387 + pntB LU632_RS11430 0.62 +1.2 2,181,388 - pntB LU632_RS11430 0.62 +1.0 2,181,427 - pntB LU632_RS11430 0.65 -0.4 2,181,600 - pntB LU632_RS11430 0.77 -0.2 2,181,887 + -0.9 2,181,887 + +1.0 2,181,887 + +0.6 2,181,887 + +0.2 2,181,887 + -1.3 2,181,888 - +0.1 2,181,898 + +0.8 2,181,902 - -0.1 2,182,140 + pntA LU632_RS11435 0.14 +0.5 2,182,141 - pntA LU632_RS11435 0.14 +0.4 2,182,141 - pntA LU632_RS11435 0.14 +0.2 2,182,151 + pntA LU632_RS11435 0.15 +1.6 2,182,169 + pntA LU632_RS11435 0.16 -0.1 2,182,442 - pntA LU632_RS11435 0.34 +1.0 2,182,515 - pntA LU632_RS11435 0.38 +0.2 2,182,713 - pntA LU632_RS11435 0.51 -0.8 2,183,099 - pntA LU632_RS11435 0.77 +0.0 2,183,157 - pntA LU632_RS11435 0.80 -0.8 2,183,189 - pntA LU632_RS11435 0.82 -0.5 2,183,189 - pntA LU632_RS11435 0.82 +0.0 2,183,254 + pntA LU632_RS11435 0.87 -0.3 2,183,271 - pntA LU632_RS11435 0.88 +1.2 2,183,311 - +2.0 2,183,454 - +1.0 2,183,458 + -0.2 2,183,627 - -0.1 2,183,695 + -0.2
Or see this region's nucleotide sequence