Experiment: HBM-CucXylem
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisF and hisA overlap by 19 nucleotides hisA and hisH are separated by 5 nucleotides hisH and hisB are separated by 0 nucleotides
LU632_RS08920: hisF - imidazole glycerol phosphate synthase subunit HisF, at 1,684,087 to 1,684,863
hisF
LU632_RS08925: hisA - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 1,684,845 to 1,685,582
hisA
LU632_RS08930: hisH - imidazole glycerol phosphate synthase subunit HisH, at 1,685,588 to 1,686,178
hisH
LU632_RS08935: hisB - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 1,686,179 to 1,687,246
hisB
Position (kb)
1685
1686
1687 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1684.589 kb on - strand, within hisF at 1684.589 kb on - strand, within hisF at 1684.589 kb on - strand, within hisF at 1684.801 kb on + strand at 1684.866 kb on + strand at 1684.866 kb on + strand at 1684.866 kb on + strand at 1685.234 kb on + strand, within hisA at 1685.234 kb on + strand, within hisA at 1685.471 kb on - strand, within hisA at 1685.478 kb on + strand, within hisA at 1685.503 kb on + strand, within hisA at 1685.576 kb on + strand at 1685.589 kb on + strand at 1685.590 kb on - strand at 1685.703 kb on + strand, within hisH at 1685.703 kb on + strand, within hisH at 1685.703 kb on + strand, within hisH at 1685.704 kb on - strand, within hisH at 1685.704 kb on - strand, within hisH at 1685.707 kb on + strand, within hisH at 1685.708 kb on - strand, within hisH at 1685.708 kb on - strand, within hisH at 1685.708 kb on - strand, within hisH at 1685.708 kb on - strand, within hisH at 1685.708 kb on - strand, within hisH at 1685.745 kb on + strand, within hisH at 1685.745 kb on + strand, within hisH at 1685.746 kb on - strand, within hisH at 1685.746 kb on - strand, within hisH at 1685.746 kb on - strand, within hisH at 1685.760 kb on + strand, within hisH at 1685.766 kb on + strand, within hisH at 1685.766 kb on + strand, within hisH at 1685.767 kb on - strand, within hisH at 1685.767 kb on - strand, within hisH at 1685.767 kb on - strand, within hisH at 1686.181 kb on - strand at 1686.181 kb on - strand at 1686.368 kb on + strand, within hisB at 1686.391 kb on + strand, within hisB at 1686.403 kb on + strand, within hisB at 1686.403 kb on + strand, within hisB at 1686.403 kb on + strand, within hisB at 1686.404 kb on - strand, within hisB at 1686.405 kb on + strand, within hisB at 1686.405 kb on + strand, within hisB at 1686.573 kb on + strand, within hisB at 1686.574 kb on - strand, within hisB at 1686.574 kb on - strand, within hisB at 1686.595 kb on + strand, within hisB at 1686.638 kb on - strand, within hisB at 1686.638 kb on - strand, within hisB at 1686.638 kb on - strand, within hisB at 1686.638 kb on - strand, within hisB at 1686.638 kb on - strand, within hisB at 1686.638 kb on - strand, within hisB at 1686.725 kb on - strand, within hisB at 1686.769 kb on + strand, within hisB at 1686.805 kb on - strand, within hisB at 1686.827 kb on - strand, within hisB at 1686.961 kb on + strand, within hisB at 1686.961 kb on + strand, within hisB at 1686.961 kb on + strand, within hisB at 1686.962 kb on - strand, within hisB at 1686.995 kb on - strand, within hisB at 1687.162 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-CucXylem remove 1,684,589 - hisF LU632_RS08920 0.65 +0.6 1,684,589 - hisF LU632_RS08920 0.65 +0.7 1,684,589 - hisF LU632_RS08920 0.65 -0.2 1,684,801 + -1.8 1,684,866 + -1.2 1,684,866 + -0.2 1,684,866 + -0.2 1,685,234 + hisA LU632_RS08925 0.53 -0.9 1,685,234 + hisA LU632_RS08925 0.53 -0.2 1,685,471 - hisA LU632_RS08925 0.85 -1.1 1,685,478 + hisA LU632_RS08925 0.86 -1.9 1,685,503 + hisA LU632_RS08925 0.89 -0.2 1,685,576 + -1.8 1,685,589 + -0.2 1,685,590 - -0.2 1,685,703 + hisH LU632_RS08930 0.19 -1.2 1,685,703 + hisH LU632_RS08930 0.19 -1.3 1,685,703 + hisH LU632_RS08930 0.19 -0.6 1,685,704 - hisH LU632_RS08930 0.20 -1.6 1,685,704 - hisH LU632_RS08930 0.20 -1.1 1,685,707 + hisH LU632_RS08930 0.20 +0.1 1,685,708 - hisH LU632_RS08930 0.20 -0.5 1,685,708 - hisH LU632_RS08930 0.20 -2.1 1,685,708 - hisH LU632_RS08930 0.20 -2.1 1,685,708 - hisH LU632_RS08930 0.20 -0.9 1,685,708 - hisH LU632_RS08930 0.20 -1.5 1,685,745 + hisH LU632_RS08930 0.27 -0.9 1,685,745 + hisH LU632_RS08930 0.27 -1.1 1,685,746 - hisH LU632_RS08930 0.27 -1.2 1,685,746 - hisH LU632_RS08930 0.27 -1.7 1,685,746 - hisH LU632_RS08930 0.27 -1.2 1,685,760 + hisH LU632_RS08930 0.29 -1.1 1,685,766 + hisH LU632_RS08930 0.30 +2.4 1,685,766 + hisH LU632_RS08930 0.30 +0.4 1,685,767 - hisH LU632_RS08930 0.30 -0.8 1,685,767 - hisH LU632_RS08930 0.30 -1.6 1,685,767 - hisH LU632_RS08930 0.30 -1.5 1,686,181 - -1.6 1,686,181 - -0.7 1,686,368 + hisB LU632_RS08935 0.18 -0.9 1,686,391 + hisB LU632_RS08935 0.20 -0.6 1,686,403 + hisB LU632_RS08935 0.21 +0.3 1,686,403 + hisB LU632_RS08935 0.21 -1.1 1,686,403 + hisB LU632_RS08935 0.21 -0.5 1,686,404 - hisB LU632_RS08935 0.21 +1.4 1,686,405 + hisB LU632_RS08935 0.21 -2.2 1,686,405 + hisB LU632_RS08935 0.21 -0.8 1,686,573 + hisB LU632_RS08935 0.37 -0.7 1,686,574 - hisB LU632_RS08935 0.37 -0.5 1,686,574 - hisB LU632_RS08935 0.37 +0.4 1,686,595 + hisB LU632_RS08935 0.39 -1.5 1,686,638 - hisB LU632_RS08935 0.43 +0.1 1,686,638 - hisB LU632_RS08935 0.43 -1.4 1,686,638 - hisB LU632_RS08935 0.43 -0.3 1,686,638 - hisB LU632_RS08935 0.43 -0.1 1,686,638 - hisB LU632_RS08935 0.43 -0.6 1,686,638 - hisB LU632_RS08935 0.43 -0.6 1,686,725 - hisB LU632_RS08935 0.51 +1.1 1,686,769 + hisB LU632_RS08935 0.55 -0.8 1,686,805 - hisB LU632_RS08935 0.59 -1.5 1,686,827 - hisB LU632_RS08935 0.61 +1.4 1,686,961 + hisB LU632_RS08935 0.73 -1.1 1,686,961 + hisB LU632_RS08935 0.73 -1.2 1,686,961 + hisB LU632_RS08935 0.73 -2.1 1,686,962 - hisB LU632_RS08935 0.73 -0.8 1,686,995 - hisB LU632_RS08935 0.76 -0.7 1,687,162 + -1.0
Or see this region's nucleotide sequence