Experiment: HBM-CucXylem
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysJ and cysI overlap by 1 nucleotides cysI and LU632_RS06410 are separated by 12 nucleotides
LU632_RS06400: cysJ - NADPH-dependent assimilatory sulfite reductase flavoprotein subunit, at 1,229,539 to 1,231,332
cysJ
LU632_RS06405: cysI - assimilatory sulfite reductase (NADPH) hemoprotein subunit, at 1,231,332 to 1,233,047
cysI
LU632_RS06410: LU632_RS06410 - phosphoadenylyl-sulfate reductase, at 1,233,060 to 1,233,791
_RS06410
Position (kb)
1231
1232
1233
1234 Strain fitness (log2 ratio)
-2
-1
0
1 at 1230.340 kb on + strand, within cysJ at 1230.370 kb on + strand, within cysJ at 1230.592 kb on + strand, within cysJ at 1230.619 kb on - strand, within cysJ at 1230.619 kb on - strand, within cysJ at 1230.638 kb on + strand, within cysJ at 1230.695 kb on - strand, within cysJ at 1230.739 kb on - strand, within cysJ at 1230.841 kb on - strand, within cysJ at 1230.855 kb on + strand, within cysJ at 1230.856 kb on - strand, within cysJ at 1230.861 kb on + strand, within cysJ at 1231.051 kb on - strand, within cysJ at 1231.105 kb on - strand, within cysJ at 1231.111 kb on + strand, within cysJ at 1231.176 kb on + strand at 1231.176 kb on + strand at 1231.176 kb on + strand at 1231.177 kb on - strand at 1231.177 kb on - strand at 1231.177 kb on - strand at 1231.327 kb on + strand at 1231.413 kb on + strand at 1231.413 kb on + strand at 1231.502 kb on + strand at 1231.502 kb on + strand at 1231.503 kb on - strand at 1231.506 kb on + strand, within cysI at 1231.506 kb on + strand, within cysI at 1231.506 kb on + strand, within cysI at 1231.507 kb on - strand, within cysI at 1231.507 kb on - strand, within cysI at 1231.507 kb on - strand, within cysI at 1231.634 kb on + strand, within cysI at 1231.725 kb on - strand, within cysI at 1231.786 kb on + strand, within cysI at 1231.787 kb on - strand, within cysI at 1231.965 kb on + strand, within cysI at 1231.983 kb on - strand, within cysI at 1232.046 kb on - strand, within cysI at 1232.051 kb on + strand, within cysI at 1232.120 kb on + strand, within cysI at 1232.121 kb on - strand, within cysI at 1232.121 kb on - strand, within cysI at 1232.121 kb on - strand, within cysI at 1232.123 kb on + strand, within cysI at 1232.134 kb on - strand, within cysI at 1232.259 kb on + strand, within cysI at 1232.610 kb on - strand, within cysI at 1232.747 kb on + strand, within cysI at 1232.747 kb on + strand, within cysI at 1232.748 kb on - strand, within cysI at 1232.835 kb on + strand, within cysI at 1232.838 kb on - strand, within cysI at 1232.873 kb on + strand, within cysI at 1232.883 kb on - strand at 1232.921 kb on + strand at 1233.318 kb on + strand, within LU632_RS06410 at 1233.451 kb on - strand, within LU632_RS06410 at 1233.684 kb on + strand, within LU632_RS06410 at 1233.702 kb on - strand, within LU632_RS06410 at 1233.789 kb on + strand at 1233.867 kb on - strand at 1233.867 kb on - strand at 1233.867 kb on - strand at 1233.963 kb on + strand at 1234.008 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-CucXylem remove 1,230,340 + cysJ LU632_RS06400 0.45 -1.4 1,230,370 + cysJ LU632_RS06400 0.46 +0.5 1,230,592 + cysJ LU632_RS06400 0.59 -0.8 1,230,619 - cysJ LU632_RS06400 0.60 -0.0 1,230,619 - cysJ LU632_RS06400 0.60 -0.5 1,230,638 + cysJ LU632_RS06400 0.61 +0.2 1,230,695 - cysJ LU632_RS06400 0.64 -2.6 1,230,739 - cysJ LU632_RS06400 0.67 -0.6 1,230,841 - cysJ LU632_RS06400 0.73 +1.5 1,230,855 + cysJ LU632_RS06400 0.73 -1.8 1,230,856 - cysJ LU632_RS06400 0.73 -0.9 1,230,861 + cysJ LU632_RS06400 0.74 -0.8 1,231,051 - cysJ LU632_RS06400 0.84 -1.5 1,231,105 - cysJ LU632_RS06400 0.87 +0.5 1,231,111 + cysJ LU632_RS06400 0.88 -0.0 1,231,176 + -0.0 1,231,176 + +0.4 1,231,176 + -0.8 1,231,177 - -2.2 1,231,177 - -1.9 1,231,177 - -0.2 1,231,327 + +1.5 1,231,413 + +0.5 1,231,413 + -0.7 1,231,502 + -1.5 1,231,502 + -1.8 1,231,503 - -1.5 1,231,506 + cysI LU632_RS06405 0.10 -0.2 1,231,506 + cysI LU632_RS06405 0.10 -1.8 1,231,506 + cysI LU632_RS06405 0.10 -0.3 1,231,507 - cysI LU632_RS06405 0.10 +0.1 1,231,507 - cysI LU632_RS06405 0.10 -1.0 1,231,507 - cysI LU632_RS06405 0.10 +0.5 1,231,634 + cysI LU632_RS06405 0.18 +0.4 1,231,725 - cysI LU632_RS06405 0.23 +0.3 1,231,786 + cysI LU632_RS06405 0.26 -0.4 1,231,787 - cysI LU632_RS06405 0.27 -2.2 1,231,965 + cysI LU632_RS06405 0.37 +0.2 1,231,983 - cysI LU632_RS06405 0.38 +0.2 1,232,046 - cysI LU632_RS06405 0.42 +0.5 1,232,051 + cysI LU632_RS06405 0.42 -0.8 1,232,120 + cysI LU632_RS06405 0.46 -0.0 1,232,121 - cysI LU632_RS06405 0.46 -2.2 1,232,121 - cysI LU632_RS06405 0.46 -1.9 1,232,121 - cysI LU632_RS06405 0.46 -1.6 1,232,123 + cysI LU632_RS06405 0.46 -0.0 1,232,134 - cysI LU632_RS06405 0.47 -2.5 1,232,259 + cysI LU632_RS06405 0.54 -0.8 1,232,610 - cysI LU632_RS06405 0.74 -1.6 1,232,747 + cysI LU632_RS06405 0.82 -0.7 1,232,747 + cysI LU632_RS06405 0.82 -0.4 1,232,748 - cysI LU632_RS06405 0.83 -2.4 1,232,835 + cysI LU632_RS06405 0.88 -0.2 1,232,838 - cysI LU632_RS06405 0.88 -1.8 1,232,873 + cysI LU632_RS06405 0.90 -1.1 1,232,883 - -0.8 1,232,921 + +0.1 1,233,318 + LU632_RS06410 0.35 -1.1 1,233,451 - LU632_RS06410 0.53 +0.2 1,233,684 + LU632_RS06410 0.85 -0.6 1,233,702 - LU632_RS06410 0.88 +0.5 1,233,789 + +0.7 1,233,867 - +0.1 1,233,867 - -0.6 1,233,867 - -0.3 1,233,963 + -1.2 1,234,008 - -0.7
Or see this region's nucleotide sequence