Experiment: HBM-CucXylem
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt queE and queD are separated by 16 nucleotides queD and cysJ are separated by 305 nucleotides cysJ and cysI overlap by 1 nucleotides
LU632_RS06390: queE - 7-carboxy-7-deazaguanine synthase QueE, at 1,228,183 to 1,228,854
queE
LU632_RS06395: queD - 6-carboxytetrahydropterin synthase QueD, at 1,228,871 to 1,229,233
queD
LU632_RS06400: cysJ - NADPH-dependent assimilatory sulfite reductase flavoprotein subunit, at 1,229,539 to 1,231,332
cysJ
LU632_RS06405: cysI - assimilatory sulfite reductase (NADPH) hemoprotein subunit, at 1,231,332 to 1,233,047
cysI
Position (kb)
1229
1230
1231
1232 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1228.552 kb on - strand, within queE at 1228.552 kb on - strand, within queE at 1228.602 kb on + strand, within queE at 1228.603 kb on - strand, within queE at 1228.603 kb on - strand, within queE at 1228.655 kb on + strand, within queE at 1228.655 kb on + strand, within queE at 1228.656 kb on - strand, within queE at 1228.786 kb on - strand, within queE at 1228.806 kb on + strand at 1228.834 kb on - strand at 1228.837 kb on + strand at 1228.842 kb on + strand at 1228.843 kb on - strand at 1228.845 kb on + strand at 1228.845 kb on + strand at 1229.009 kb on - strand, within queD at 1229.019 kb on + strand, within queD at 1229.019 kb on + strand, within queD at 1229.019 kb on + strand, within queD at 1229.020 kb on - strand, within queD at 1229.020 kb on - strand, within queD at 1229.020 kb on - strand, within queD at 1229.020 kb on - strand, within queD at 1229.020 kb on - strand, within queD at 1229.040 kb on + strand, within queD at 1229.040 kb on + strand, within queD at 1229.040 kb on + strand, within queD at 1229.040 kb on + strand, within queD at 1229.041 kb on - strand, within queD at 1229.043 kb on - strand, within queD at 1229.131 kb on + strand, within queD at 1229.132 kb on - strand, within queD at 1229.162 kb on - strand, within queD at 1229.162 kb on - strand, within queD at 1229.219 kb on + strand at 1229.396 kb on - strand at 1229.435 kb on + strand at 1229.451 kb on + strand at 1229.465 kb on + strand at 1229.466 kb on - strand at 1229.653 kb on + strand at 1229.653 kb on + strand at 1229.654 kb on - strand at 1229.724 kb on - strand, within cysJ at 1229.784 kb on - strand, within cysJ at 1229.799 kb on + strand, within cysJ at 1229.800 kb on - strand, within cysJ at 1229.829 kb on + strand, within cysJ at 1229.998 kb on + strand, within cysJ at 1230.282 kb on + strand, within cysJ at 1230.282 kb on + strand, within cysJ at 1230.340 kb on + strand, within cysJ at 1230.370 kb on + strand, within cysJ at 1230.592 kb on + strand, within cysJ at 1230.619 kb on - strand, within cysJ at 1230.619 kb on - strand, within cysJ at 1230.638 kb on + strand, within cysJ at 1230.695 kb on - strand, within cysJ at 1230.739 kb on - strand, within cysJ at 1230.841 kb on - strand, within cysJ at 1230.855 kb on + strand, within cysJ at 1230.856 kb on - strand, within cysJ at 1230.861 kb on + strand, within cysJ at 1231.051 kb on - strand, within cysJ at 1231.105 kb on - strand, within cysJ at 1231.111 kb on + strand, within cysJ at 1231.176 kb on + strand at 1231.176 kb on + strand at 1231.176 kb on + strand at 1231.177 kb on - strand at 1231.177 kb on - strand at 1231.177 kb on - strand at 1231.327 kb on + strand at 1231.413 kb on + strand at 1231.413 kb on + strand at 1231.502 kb on + strand at 1231.502 kb on + strand at 1231.503 kb on - strand at 1231.506 kb on + strand, within cysI at 1231.506 kb on + strand, within cysI at 1231.506 kb on + strand, within cysI at 1231.507 kb on - strand, within cysI at 1231.507 kb on - strand, within cysI at 1231.507 kb on - strand, within cysI at 1231.634 kb on + strand, within cysI at 1231.725 kb on - strand, within cysI at 1231.786 kb on + strand, within cysI at 1231.787 kb on - strand, within cysI at 1231.965 kb on + strand, within cysI at 1231.983 kb on - strand, within cysI at 1232.046 kb on - strand, within cysI at 1232.051 kb on + strand, within cysI at 1232.120 kb on + strand, within cysI at 1232.121 kb on - strand, within cysI at 1232.121 kb on - strand, within cysI at 1232.121 kb on - strand, within cysI at 1232.123 kb on + strand, within cysI at 1232.134 kb on - strand, within cysI at 1232.259 kb on + strand, within cysI
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-CucXylem remove 1,228,552 - queE LU632_RS06390 0.55 +1.5 1,228,552 - queE LU632_RS06390 0.55 +1.5 1,228,602 + queE LU632_RS06390 0.62 +0.2 1,228,603 - queE LU632_RS06390 0.62 +1.0 1,228,603 - queE LU632_RS06390 0.62 +0.5 1,228,655 + queE LU632_RS06390 0.70 +0.6 1,228,655 + queE LU632_RS06390 0.70 -1.0 1,228,656 - queE LU632_RS06390 0.70 -0.1 1,228,786 - queE LU632_RS06390 0.90 +0.5 1,228,806 + -0.3 1,228,834 - -0.2 1,228,837 + -0.0 1,228,842 + +0.5 1,228,843 - -0.8 1,228,845 + -0.5 1,228,845 + -0.9 1,229,009 - queD LU632_RS06395 0.38 +0.9 1,229,019 + queD LU632_RS06395 0.41 +1.0 1,229,019 + queD LU632_RS06395 0.41 -0.1 1,229,019 + queD LU632_RS06395 0.41 -2.5 1,229,020 - queD LU632_RS06395 0.41 +1.5 1,229,020 - queD LU632_RS06395 0.41 +0.0 1,229,020 - queD LU632_RS06395 0.41 +0.2 1,229,020 - queD LU632_RS06395 0.41 -0.2 1,229,020 - queD LU632_RS06395 0.41 -0.0 1,229,040 + queD LU632_RS06395 0.47 +0.7 1,229,040 + queD LU632_RS06395 0.47 +0.5 1,229,040 + queD LU632_RS06395 0.47 -0.7 1,229,040 + queD LU632_RS06395 0.47 -1.3 1,229,041 - queD LU632_RS06395 0.47 +1.9 1,229,043 - queD LU632_RS06395 0.47 -0.5 1,229,131 + queD LU632_RS06395 0.72 -0.0 1,229,132 - queD LU632_RS06395 0.72 -1.2 1,229,162 - queD LU632_RS06395 0.80 +0.2 1,229,162 - queD LU632_RS06395 0.80 -0.0 1,229,219 + -0.7 1,229,396 - -1.6 1,229,435 + +0.1 1,229,451 + -0.0 1,229,465 + +0.3 1,229,466 - -1.0 1,229,653 + +0.1 1,229,653 + -1.7 1,229,654 - -0.9 1,229,724 - cysJ LU632_RS06400 0.10 +1.1 1,229,784 - cysJ LU632_RS06400 0.14 -1.8 1,229,799 + cysJ LU632_RS06400 0.14 -0.3 1,229,800 - cysJ LU632_RS06400 0.15 -1.3 1,229,829 + cysJ LU632_RS06400 0.16 -0.4 1,229,998 + cysJ LU632_RS06400 0.26 +0.5 1,230,282 + cysJ LU632_RS06400 0.41 +0.5 1,230,282 + cysJ LU632_RS06400 0.41 -2.2 1,230,340 + cysJ LU632_RS06400 0.45 -1.4 1,230,370 + cysJ LU632_RS06400 0.46 +0.5 1,230,592 + cysJ LU632_RS06400 0.59 -0.8 1,230,619 - cysJ LU632_RS06400 0.60 -0.0 1,230,619 - cysJ LU632_RS06400 0.60 -0.5 1,230,638 + cysJ LU632_RS06400 0.61 +0.2 1,230,695 - cysJ LU632_RS06400 0.64 -2.6 1,230,739 - cysJ LU632_RS06400 0.67 -0.6 1,230,841 - cysJ LU632_RS06400 0.73 +1.5 1,230,855 + cysJ LU632_RS06400 0.73 -1.8 1,230,856 - cysJ LU632_RS06400 0.73 -0.9 1,230,861 + cysJ LU632_RS06400 0.74 -0.8 1,231,051 - cysJ LU632_RS06400 0.84 -1.5 1,231,105 - cysJ LU632_RS06400 0.87 +0.5 1,231,111 + cysJ LU632_RS06400 0.88 -0.0 1,231,176 + -0.0 1,231,176 + +0.4 1,231,176 + -0.8 1,231,177 - -2.2 1,231,177 - -1.9 1,231,177 - -0.2 1,231,327 + +1.5 1,231,413 + +0.5 1,231,413 + -0.7 1,231,502 + -1.5 1,231,502 + -1.8 1,231,503 - -1.5 1,231,506 + cysI LU632_RS06405 0.10 -0.2 1,231,506 + cysI LU632_RS06405 0.10 -1.8 1,231,506 + cysI LU632_RS06405 0.10 -0.3 1,231,507 - cysI LU632_RS06405 0.10 +0.1 1,231,507 - cysI LU632_RS06405 0.10 -1.0 1,231,507 - cysI LU632_RS06405 0.10 +0.5 1,231,634 + cysI LU632_RS06405 0.18 +0.4 1,231,725 - cysI LU632_RS06405 0.23 +0.3 1,231,786 + cysI LU632_RS06405 0.26 -0.4 1,231,787 - cysI LU632_RS06405 0.27 -2.2 1,231,965 + cysI LU632_RS06405 0.37 +0.2 1,231,983 - cysI LU632_RS06405 0.38 +0.2 1,232,046 - cysI LU632_RS06405 0.42 +0.5 1,232,051 + cysI LU632_RS06405 0.42 -0.8 1,232,120 + cysI LU632_RS06405 0.46 -0.0 1,232,121 - cysI LU632_RS06405 0.46 -2.2 1,232,121 - cysI LU632_RS06405 0.46 -1.9 1,232,121 - cysI LU632_RS06405 0.46 -1.6 1,232,123 + cysI LU632_RS06405 0.46 -0.0 1,232,134 - cysI LU632_RS06405 0.47 -2.5 1,232,259 + cysI LU632_RS06405 0.54 -0.8
Or see this region's nucleotide sequence