Experiment: HBM-CucXylem
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pdxA and rsmA overlap by 1 nucleotides rsmA and apaG are separated by 1 nucleotides apaG and apaH are separated by 44 nucleotides
LU632_RS02725: pdxA - 4-hydroxythreonine-4-phosphate dehydrogenase PdxA, at 548,981 to 549,964
pdxA
LU632_RS02730: rsmA - 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA, at 549,964 to 550,794
rsmA
LU632_RS02735: apaG - Co2+/Mg2+ efflux protein ApaG, at 550,796 to 551,173
apaG
LU632_RS02740: apaH - bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH, at 551,218 to 552,048
apaH
Position (kb) 550
551
552 Strain fitness (log2 ratio) -2
-1
0
1 at 549.829 kb on + strand, within pdxA at 549.934 kb on + strand at 549.962 kb on + strand at 550.033 kb on + strand at 550.034 kb on - strand at 550.036 kb on - strand at 550.144 kb on - strand, within rsmA at 550.261 kb on + strand, within rsmA at 550.309 kb on + strand, within rsmA at 550.310 kb on - strand, within rsmA at 550.311 kb on + strand, within rsmA at 550.349 kb on - strand, within rsmA at 550.351 kb on - strand, within rsmA at 550.459 kb on + strand, within rsmA at 550.538 kb on + strand, within rsmA at 550.629 kb on + strand, within rsmA at 550.835 kb on - strand, within apaG at 550.874 kb on + strand, within apaG at 550.888 kb on + strand, within apaG at 550.888 kb on + strand, within apaG at 550.929 kb on + strand, within apaG at 551.039 kb on + strand, within apaG at 551.092 kb on + strand, within apaG at 551.092 kb on + strand, within apaG at 551.092 kb on + strand, within apaG at 551.092 kb on + strand, within apaG at 551.092 kb on + strand, within apaG at 551.093 kb on - strand, within apaG at 551.093 kb on - strand, within apaG at 551.093 kb on - strand, within apaG at 551.093 kb on - strand, within apaG at 551.127 kb on + strand, within apaG at 551.172 kb on - strand at 552.050 kb on + strand at 552.065 kb on + strand at 552.065 kb on + strand at 552.065 kb on + strand at 552.065 kb on + strand at 552.066 kb on - strand at 552.088 kb on + strand at 552.088 kb on + strand at 552.088 kb on + strand at 552.088 kb on + strand at 552.089 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-CucXylem remove 549,829 + pdxA LU632_RS02725 0.86 +0.7 549,934 + +0.1 549,962 + +1.7 550,033 + +1.7 550,034 - +0.1 550,036 - -0.6 550,144 - rsmA LU632_RS02730 0.22 -1.3 550,261 + rsmA LU632_RS02730 0.36 +0.7 550,309 + rsmA LU632_RS02730 0.42 +0.7 550,310 - rsmA LU632_RS02730 0.42 -0.3 550,311 + rsmA LU632_RS02730 0.42 -1.3 550,349 - rsmA LU632_RS02730 0.46 -1.0 550,351 - rsmA LU632_RS02730 0.47 -0.6 550,459 + rsmA LU632_RS02730 0.60 -0.5 550,538 + rsmA LU632_RS02730 0.69 +1.1 550,629 + rsmA LU632_RS02730 0.80 -0.3 550,835 - apaG LU632_RS02735 0.10 -1.8 550,874 + apaG LU632_RS02735 0.21 -1.6 550,888 + apaG LU632_RS02735 0.24 -1.4 550,888 + apaG LU632_RS02735 0.24 -0.7 550,929 + apaG LU632_RS02735 0.35 -1.5 551,039 + apaG LU632_RS02735 0.64 +0.1 551,092 + apaG LU632_RS02735 0.78 -1.6 551,092 + apaG LU632_RS02735 0.78 +0.3 551,092 + apaG LU632_RS02735 0.78 -0.9 551,092 + apaG LU632_RS02735 0.78 -0.7 551,092 + apaG LU632_RS02735 0.78 -0.2 551,093 - apaG LU632_RS02735 0.79 -0.9 551,093 - apaG LU632_RS02735 0.79 -1.1 551,093 - apaG LU632_RS02735 0.79 -1.4 551,093 - apaG LU632_RS02735 0.79 +0.4 551,127 + apaG LU632_RS02735 0.88 -1.0 551,172 - -0.9 552,050 + -0.0 552,065 + -0.9 552,065 + -1.3 552,065 + +0.0 552,065 + -0.5 552,066 - -0.2 552,088 + -2.0 552,088 + -1.0 552,088 + -0.1 552,088 + +0.2 552,089 - +0.6
Or see this region's nucleotide sequence