Experiment: HBM-CucXylem
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thrA and thrB are separated by 2 nucleotides thrB and thrC are separated by 3 nucleotides
LU632_RS02610: thrA - bifunctional aspartate kinase/homoserine dehydrogenase I, at 520,215 to 522,677
thrA
LU632_RS02615: thrB - homoserine kinase, at 522,680 to 523,609
thrB
LU632_RS02620: thrC - threonine synthase, at 523,613 to 524,899
thrC
Position (kb) 520
521
522
523 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 519.395 kb on - strand at 519.405 kb on - strand at 519.587 kb on - strand at 519.620 kb on + strand at 519.662 kb on - strand at 519.764 kb on - strand at 519.784 kb on + strand at 519.785 kb on - strand at 519.809 kb on - strand at 519.863 kb on - strand at 519.884 kb on - strand at 519.979 kb on + strand at 520.023 kb on - strand at 520.049 kb on - strand at 520.056 kb on - strand at 520.071 kb on + strand at 520.095 kb on + strand at 520.099 kb on - strand at 520.099 kb on - strand at 520.111 kb on - strand at 520.140 kb on - strand at 520.395 kb on - strand at 520.403 kb on + strand at 520.528 kb on + strand, within thrA at 520.580 kb on - strand, within thrA at 520.913 kb on + strand, within thrA at 520.965 kb on + strand, within thrA at 520.989 kb on + strand, within thrA at 521.021 kb on + strand, within thrA at 521.021 kb on + strand, within thrA at 521.228 kb on + strand, within thrA at 521.229 kb on - strand, within thrA at 521.281 kb on - strand, within thrA at 521.416 kb on + strand, within thrA at 521.450 kb on - strand, within thrA at 521.700 kb on - strand, within thrA at 521.700 kb on - strand, within thrA at 521.855 kb on + strand, within thrA at 521.905 kb on - strand, within thrA at 521.976 kb on + strand, within thrA at 521.976 kb on + strand, within thrA at 521.977 kb on - strand, within thrA at 521.977 kb on - strand, within thrA at 521.977 kb on - strand, within thrA at 521.989 kb on + strand, within thrA at 521.989 kb on + strand, within thrA at 521.989 kb on + strand, within thrA at 521.994 kb on + strand, within thrA at 522.061 kb on + strand, within thrA at 522.140 kb on + strand, within thrA at 522.141 kb on - strand, within thrA at 522.141 kb on - strand, within thrA at 522.166 kb on + strand, within thrA at 522.167 kb on - strand, within thrA at 522.435 kb on - strand at 522.519 kb on + strand at 522.519 kb on + strand at 522.650 kb on + strand at 522.651 kb on - strand at 522.723 kb on + strand at 522.787 kb on + strand, within thrB at 522.842 kb on - strand, within thrB at 522.869 kb on - strand, within thrB at 523.016 kb on + strand, within thrB at 523.016 kb on + strand, within thrB at 523.017 kb on - strand, within thrB at 523.017 kb on - strand, within thrB at 523.219 kb on + strand, within thrB at 523.219 kb on + strand, within thrB at 523.262 kb on + strand, within thrB at 523.263 kb on - strand, within thrB at 523.263 kb on - strand, within thrB at 523.384 kb on + strand, within thrB at 523.427 kb on - strand, within thrB at 523.432 kb on + strand, within thrB at 523.499 kb on - strand, within thrB at 523.539 kb on - strand at 523.539 kb on - strand at 523.568 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-CucXylem remove 519,395 - -1.0 519,405 - +0.3 519,587 - -0.8 519,620 + -0.2 519,662 - -0.4 519,764 - -1.8 519,784 + +0.8 519,785 - +0.7 519,809 - -0.7 519,863 - +0.1 519,884 - -0.8 519,979 + -0.1 520,023 - -1.1 520,049 - -0.9 520,056 - -0.8 520,071 + +0.1 520,095 + +0.5 520,099 - -0.8 520,099 - +0.1 520,111 - -1.9 520,140 - -1.8 520,395 - +0.1 520,403 + -1.6 520,528 + thrA LU632_RS02610 0.13 -1.4 520,580 - thrA LU632_RS02610 0.15 -0.5 520,913 + thrA LU632_RS02610 0.28 -0.9 520,965 + thrA LU632_RS02610 0.30 -1.9 520,989 + thrA LU632_RS02610 0.31 -1.6 521,021 + thrA LU632_RS02610 0.33 +0.7 521,021 + thrA LU632_RS02610 0.33 -0.2 521,228 + thrA LU632_RS02610 0.41 -0.4 521,229 - thrA LU632_RS02610 0.41 -0.2 521,281 - thrA LU632_RS02610 0.43 -1.4 521,416 + thrA LU632_RS02610 0.49 +1.7 521,450 - thrA LU632_RS02610 0.50 -0.9 521,700 - thrA LU632_RS02610 0.60 -0.6 521,700 - thrA LU632_RS02610 0.60 +1.3 521,855 + thrA LU632_RS02610 0.67 +0.1 521,905 - thrA LU632_RS02610 0.69 -3.4 521,976 + thrA LU632_RS02610 0.71 -0.9 521,976 + thrA LU632_RS02610 0.71 -1.6 521,977 - thrA LU632_RS02610 0.72 +0.7 521,977 - thrA LU632_RS02610 0.72 +0.5 521,977 - thrA LU632_RS02610 0.72 +1.1 521,989 + thrA LU632_RS02610 0.72 -2.1 521,989 + thrA LU632_RS02610 0.72 -0.6 521,989 + thrA LU632_RS02610 0.72 -2.6 521,994 + thrA LU632_RS02610 0.72 -1.5 522,061 + thrA LU632_RS02610 0.75 -1.0 522,140 + thrA LU632_RS02610 0.78 +2.3 522,141 - thrA LU632_RS02610 0.78 -0.7 522,141 - thrA LU632_RS02610 0.78 -1.3 522,166 + thrA LU632_RS02610 0.79 -0.1 522,167 - thrA LU632_RS02610 0.79 +0.7 522,435 - +0.1 522,519 + -1.9 522,519 + -1.3 522,650 + -0.3 522,651 - +0.1 522,723 + +0.7 522,787 + thrB LU632_RS02615 0.12 -0.9 522,842 - thrB LU632_RS02615 0.17 -2.5 522,869 - thrB LU632_RS02615 0.20 -0.3 523,016 + thrB LU632_RS02615 0.36 -1.8 523,016 + thrB LU632_RS02615 0.36 +1.7 523,017 - thrB LU632_RS02615 0.36 -1.5 523,017 - thrB LU632_RS02615 0.36 +1.1 523,219 + thrB LU632_RS02615 0.58 -1.3 523,219 + thrB LU632_RS02615 0.58 -0.5 523,262 + thrB LU632_RS02615 0.63 -1.1 523,263 - thrB LU632_RS02615 0.63 -1.3 523,263 - thrB LU632_RS02615 0.63 -1.5 523,384 + thrB LU632_RS02615 0.76 -1.2 523,427 - thrB LU632_RS02615 0.80 +0.7 523,432 + thrB LU632_RS02615 0.81 -1.5 523,499 - thrB LU632_RS02615 0.88 -1.2 523,539 - -1.3 523,539 - -1.3 523,568 - -3.1
Or see this region's nucleotide sequence