Experiment: HBM-CucXylem
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS14745 and ycfP are separated by 59 nucleotides ycfP and nagZ are separated by 28 nucleotides nagZ and thiK are separated by 9 nucleotides thiK and lpoB overlap by 41 nucleotides
LU632_RS14745: LU632_RS14745 - IS5 family transposase, at 2,803,988 to 2,804,836
_RS14745
LU632_RS14750: ycfP - alpha/beta hydrolase YcfP, at 2,804,896 to 2,805,456
ycfP
LU632_RS14755: nagZ - beta-N-acetylhexosaminidase, at 2,805,485 to 2,806,513
nagZ
LU632_RS14760: thiK - thiamine kinase, at 2,806,523 to 2,807,392
thiK
LU632_RS14765: lpoB - penicillin-binding protein activator LpoB, at 2,807,352 to 2,807,918
lpoB
Position (kb)
2805
2806
2807 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2804.509 kb on + strand, within LU632_RS14745 at 2804.867 kb on + strand at 2804.869 kb on + strand at 2804.919 kb on - strand at 2804.997 kb on + strand, within ycfP at 2805.134 kb on + strand, within ycfP at 2805.134 kb on + strand, within ycfP at 2805.134 kb on + strand, within ycfP at 2805.135 kb on - strand, within ycfP at 2805.135 kb on - strand, within ycfP at 2805.135 kb on - strand, within ycfP at 2805.135 kb on - strand, within ycfP at 2805.165 kb on + strand, within ycfP at 2805.166 kb on - strand, within ycfP at 2805.166 kb on - strand, within ycfP at 2805.166 kb on - strand, within ycfP at 2805.250 kb on + strand, within ycfP at 2805.330 kb on + strand, within ycfP at 2805.330 kb on + strand, within ycfP at 2805.330 kb on + strand, within ycfP at 2805.356 kb on + strand, within ycfP at 2805.360 kb on + strand, within ycfP at 2805.370 kb on + strand, within ycfP at 2805.371 kb on - strand, within ycfP at 2805.371 kb on - strand, within ycfP at 2805.447 kb on - strand at 2805.447 kb on - strand at 2805.451 kb on + strand at 2805.452 kb on - strand at 2805.597 kb on + strand, within nagZ at 2805.597 kb on + strand, within nagZ at 2805.598 kb on - strand, within nagZ at 2805.598 kb on - strand, within nagZ at 2805.598 kb on - strand, within nagZ at 2805.638 kb on - strand, within nagZ at 2805.726 kb on + strand, within nagZ at 2805.727 kb on - strand, within nagZ at 2805.791 kb on + strand, within nagZ at 2805.792 kb on - strand, within nagZ at 2805.815 kb on - strand, within nagZ at 2805.847 kb on + strand, within nagZ at 2805.856 kb on + strand, within nagZ at 2805.856 kb on + strand, within nagZ at 2805.857 kb on - strand, within nagZ at 2805.861 kb on + strand, within nagZ at 2805.862 kb on - strand, within nagZ at 2805.866 kb on - strand, within nagZ at 2805.866 kb on - strand, within nagZ at 2805.866 kb on - strand, within nagZ at 2805.866 kb on - strand, within nagZ at 2805.866 kb on - strand, within nagZ at 2805.922 kb on + strand, within nagZ at 2806.061 kb on - strand, within nagZ at 2806.145 kb on + strand, within nagZ at 2806.166 kb on - strand, within nagZ at 2806.405 kb on + strand, within nagZ at 2806.405 kb on + strand, within nagZ at 2806.420 kb on + strand at 2806.431 kb on - strand at 2806.477 kb on - strand at 2806.490 kb on + strand at 2806.491 kb on - strand at 2806.491 kb on - strand at 2806.491 kb on - strand at 2806.491 kb on - strand at 2806.491 kb on - strand at 2806.493 kb on + strand at 2806.567 kb on + strand at 2806.568 kb on - strand at 2806.605 kb on + strand at 2806.664 kb on + strand, within thiK at 2806.664 kb on + strand, within thiK at 2806.664 kb on + strand, within thiK at 2806.664 kb on + strand, within thiK at 2806.664 kb on + strand, within thiK at 2806.695 kb on + strand, within thiK at 2806.743 kb on - strand, within thiK at 2806.743 kb on - strand, within thiK at 2806.826 kb on + strand, within thiK at 2806.826 kb on + strand, within thiK at 2806.827 kb on - strand, within thiK at 2806.827 kb on - strand, within thiK at 2806.828 kb on + strand, within thiK at 2806.829 kb on - strand, within thiK at 2806.846 kb on + strand, within thiK at 2807.056 kb on + strand, within thiK at 2807.165 kb on + strand, within thiK at 2807.166 kb on - strand, within thiK at 2807.253 kb on - strand, within thiK at 2807.288 kb on + strand, within thiK at 2807.288 kb on + strand, within thiK at 2807.291 kb on - strand, within thiK at 2807.291 kb on - strand, within thiK at 2807.453 kb on + strand, within lpoB at 2807.453 kb on + strand, within lpoB at 2807.457 kb on - strand, within lpoB at 2807.457 kb on - strand, within lpoB at 2807.458 kb on + strand, within lpoB at 2807.467 kb on + strand, within lpoB
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-CucXylem remove 2,804,509 + LU632_RS14745 0.61 -1.0 2,804,867 + -1.9 2,804,869 + +0.3 2,804,919 - +0.1 2,804,997 + ycfP LU632_RS14750 0.18 -0.9 2,805,134 + ycfP LU632_RS14750 0.42 -0.8 2,805,134 + ycfP LU632_RS14750 0.42 -0.2 2,805,134 + ycfP LU632_RS14750 0.42 -1.3 2,805,135 - ycfP LU632_RS14750 0.43 -1.1 2,805,135 - ycfP LU632_RS14750 0.43 +0.6 2,805,135 - ycfP LU632_RS14750 0.43 +1.3 2,805,135 - ycfP LU632_RS14750 0.43 -0.9 2,805,165 + ycfP LU632_RS14750 0.48 +0.1 2,805,166 - ycfP LU632_RS14750 0.48 +1.0 2,805,166 - ycfP LU632_RS14750 0.48 +0.4 2,805,166 - ycfP LU632_RS14750 0.48 -1.3 2,805,250 + ycfP LU632_RS14750 0.63 +1.0 2,805,330 + ycfP LU632_RS14750 0.77 -0.2 2,805,330 + ycfP LU632_RS14750 0.77 +0.9 2,805,330 + ycfP LU632_RS14750 0.77 -1.0 2,805,356 + ycfP LU632_RS14750 0.82 +0.2 2,805,360 + ycfP LU632_RS14750 0.83 -0.7 2,805,370 + ycfP LU632_RS14750 0.84 -1.0 2,805,371 - ycfP LU632_RS14750 0.85 +0.2 2,805,371 - ycfP LU632_RS14750 0.85 +0.2 2,805,447 - -0.3 2,805,447 - +0.2 2,805,451 + +0.8 2,805,452 - +0.2 2,805,597 + nagZ LU632_RS14755 0.11 +1.6 2,805,597 + nagZ LU632_RS14755 0.11 +0.3 2,805,598 - nagZ LU632_RS14755 0.11 -0.0 2,805,598 - nagZ LU632_RS14755 0.11 -1.0 2,805,598 - nagZ LU632_RS14755 0.11 +0.2 2,805,638 - nagZ LU632_RS14755 0.15 -0.6 2,805,726 + nagZ LU632_RS14755 0.23 -1.9 2,805,727 - nagZ LU632_RS14755 0.24 -0.3 2,805,791 + nagZ LU632_RS14755 0.30 -0.2 2,805,792 - nagZ LU632_RS14755 0.30 +0.8 2,805,815 - nagZ LU632_RS14755 0.32 +1.0 2,805,847 + nagZ LU632_RS14755 0.35 +0.2 2,805,856 + nagZ LU632_RS14755 0.36 -0.0 2,805,856 + nagZ LU632_RS14755 0.36 -0.8 2,805,857 - nagZ LU632_RS14755 0.36 +0.7 2,805,861 + nagZ LU632_RS14755 0.37 -1.6 2,805,862 - nagZ LU632_RS14755 0.37 -0.6 2,805,866 - nagZ LU632_RS14755 0.37 -0.2 2,805,866 - nagZ LU632_RS14755 0.37 -0.3 2,805,866 - nagZ LU632_RS14755 0.37 +2.0 2,805,866 - nagZ LU632_RS14755 0.37 +0.3 2,805,866 - nagZ LU632_RS14755 0.37 -0.2 2,805,922 + nagZ LU632_RS14755 0.42 -0.6 2,806,061 - nagZ LU632_RS14755 0.56 -0.8 2,806,145 + nagZ LU632_RS14755 0.64 +0.3 2,806,166 - nagZ LU632_RS14755 0.66 +0.3 2,806,405 + nagZ LU632_RS14755 0.89 -0.6 2,806,405 + nagZ LU632_RS14755 0.89 -0.6 2,806,420 + -0.0 2,806,431 - -1.3 2,806,477 - -0.4 2,806,490 + +0.2 2,806,491 - +0.2 2,806,491 - -0.1 2,806,491 - -0.0 2,806,491 - -0.4 2,806,491 - +0.2 2,806,493 + -0.6 2,806,567 + +1.0 2,806,568 - -0.5 2,806,605 + +0.3 2,806,664 + thiK LU632_RS14760 0.16 -0.8 2,806,664 + thiK LU632_RS14760 0.16 -0.1 2,806,664 + thiK LU632_RS14760 0.16 -0.6 2,806,664 + thiK LU632_RS14760 0.16 +0.3 2,806,664 + thiK LU632_RS14760 0.16 -0.2 2,806,695 + thiK LU632_RS14760 0.20 +1.6 2,806,743 - thiK LU632_RS14760 0.25 -0.8 2,806,743 - thiK LU632_RS14760 0.25 +0.2 2,806,826 + thiK LU632_RS14760 0.35 +0.2 2,806,826 + thiK LU632_RS14760 0.35 +0.4 2,806,827 - thiK LU632_RS14760 0.35 +0.9 2,806,827 - thiK LU632_RS14760 0.35 +0.5 2,806,828 + thiK LU632_RS14760 0.35 -0.4 2,806,829 - thiK LU632_RS14760 0.35 +0.3 2,806,846 + thiK LU632_RS14760 0.37 +1.1 2,807,056 + thiK LU632_RS14760 0.61 -1.1 2,807,165 + thiK LU632_RS14760 0.74 +1.0 2,807,166 - thiK LU632_RS14760 0.74 +0.6 2,807,253 - thiK LU632_RS14760 0.84 -0.0 2,807,288 + thiK LU632_RS14760 0.88 -0.4 2,807,288 + thiK LU632_RS14760 0.88 +0.2 2,807,291 - thiK LU632_RS14760 0.88 -0.2 2,807,291 - thiK LU632_RS14760 0.88 -0.0 2,807,453 + lpoB LU632_RS14765 0.18 -0.1 2,807,453 + lpoB LU632_RS14765 0.18 -0.6 2,807,457 - lpoB LU632_RS14765 0.19 -0.6 2,807,457 - lpoB LU632_RS14765 0.19 -0.4 2,807,458 + lpoB LU632_RS14765 0.19 -0.5 2,807,467 + lpoB LU632_RS14765 0.20 +0.0
Or see this region's nucleotide sequence