Experiment: R2A with 6-Pentyl-2H-pyran-2-one 0.2865 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS22945 and ABIE41_RS22950 are separated by 91 nucleotides ABIE41_RS22950 and ABIE41_RS22955 are separated by 139 nucleotides
ABIE41_RS22945: ABIE41_RS22945 - cytochrome c oxidase subunit II, at 4,725,463 to 4,726,248
_RS22945
ABIE41_RS22950: ABIE41_RS22950 - cytochrome c oxidase subunit I, at 4,726,340 to 4,727,971
_RS22950
ABIE41_RS22955: ABIE41_RS22955 - heme o synthase, at 4,728,111 to 4,729,058
_RS22955
Position (kb)
4726
4727
4728 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 4725.517 kb on + strand at 4725.592 kb on + strand, within ABIE41_RS22945 at 4725.592 kb on + strand, within ABIE41_RS22945 at 4725.592 kb on + strand, within ABIE41_RS22945 at 4725.648 kb on + strand, within ABIE41_RS22945 at 4725.649 kb on - strand, within ABIE41_RS22945 at 4725.799 kb on + strand, within ABIE41_RS22945 at 4725.799 kb on + strand, within ABIE41_RS22945 at 4725.800 kb on - strand, within ABIE41_RS22945 at 4726.084 kb on + strand, within ABIE41_RS22945 at 4726.084 kb on + strand, within ABIE41_RS22945 at 4726.084 kb on + strand, within ABIE41_RS22945 at 4726.085 kb on - strand, within ABIE41_RS22945 at 4726.088 kb on - strand, within ABIE41_RS22945 at 4726.126 kb on + strand, within ABIE41_RS22945 at 4726.126 kb on + strand, within ABIE41_RS22945 at 4726.126 kb on + strand, within ABIE41_RS22945 at 4726.127 kb on - strand, within ABIE41_RS22945 at 4726.165 kb on + strand, within ABIE41_RS22945 at 4726.166 kb on - strand, within ABIE41_RS22945 at 4726.458 kb on - strand at 4726.565 kb on + strand, within ABIE41_RS22950 at 4726.566 kb on - strand, within ABIE41_RS22950 at 4726.806 kb on - strand, within ABIE41_RS22950 at 4727.076 kb on - strand, within ABIE41_RS22950 at 4727.076 kb on - strand, within ABIE41_RS22950 at 4727.222 kb on + strand, within ABIE41_RS22950 at 4727.237 kb on + strand, within ABIE41_RS22950 at 4727.291 kb on + strand, within ABIE41_RS22950 at 4727.295 kb on - strand, within ABIE41_RS22950 at 4727.407 kb on + strand, within ABIE41_RS22950 at 4727.525 kb on + strand, within ABIE41_RS22950 at 4727.525 kb on + strand, within ABIE41_RS22950 at 4727.526 kb on - strand, within ABIE41_RS22950 at 4727.528 kb on + strand, within ABIE41_RS22950 at 4727.529 kb on - strand, within ABIE41_RS22950 at 4727.594 kb on + strand, within ABIE41_RS22950 at 4727.595 kb on - strand, within ABIE41_RS22950 at 4727.598 kb on - strand, within ABIE41_RS22950 at 4727.622 kb on - strand, within ABIE41_RS22950 at 4727.733 kb on - strand, within ABIE41_RS22950 at 4727.742 kb on - strand, within ABIE41_RS22950 at 4727.789 kb on + strand, within ABIE41_RS22950 at 4727.789 kb on + strand, within ABIE41_RS22950 at 4727.790 kb on - strand, within ABIE41_RS22950 at 4727.790 kb on - strand, within ABIE41_RS22950 at 4727.790 kb on - strand, within ABIE41_RS22950 at 4727.790 kb on - strand, within ABIE41_RS22950 at 4727.822 kb on + strand at 4727.970 kb on - strand at 4728.517 kb on - strand, within ABIE41_RS22955 at 4728.517 kb on - strand, within ABIE41_RS22955 at 4728.564 kb on - strand, within ABIE41_RS22955 at 4728.664 kb on + strand, within ABIE41_RS22955 at 4728.721 kb on - strand, within ABIE41_RS22955 at 4728.792 kb on + strand, within ABIE41_RS22955
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 0.2865 mM remove 4,725,517 + -1.1 4,725,592 + ABIE41_RS22945 0.16 -1.5 4,725,592 + ABIE41_RS22945 0.16 -2.0 4,725,592 + ABIE41_RS22945 0.16 -1.6 4,725,648 + ABIE41_RS22945 0.24 -2.1 4,725,649 - ABIE41_RS22945 0.24 -2.8 4,725,799 + ABIE41_RS22945 0.43 -0.8 4,725,799 + ABIE41_RS22945 0.43 -2.1 4,725,800 - ABIE41_RS22945 0.43 -3.7 4,726,084 + ABIE41_RS22945 0.79 -1.2 4,726,084 + ABIE41_RS22945 0.79 -2.0 4,726,084 + ABIE41_RS22945 0.79 -0.9 4,726,085 - ABIE41_RS22945 0.79 -3.0 4,726,088 - ABIE41_RS22945 0.80 -1.9 4,726,126 + ABIE41_RS22945 0.84 -1.1 4,726,126 + ABIE41_RS22945 0.84 -0.3 4,726,126 + ABIE41_RS22945 0.84 -1.3 4,726,127 - ABIE41_RS22945 0.84 -0.5 4,726,165 + ABIE41_RS22945 0.89 -1.7 4,726,166 - ABIE41_RS22945 0.89 -2.9 4,726,458 - -2.5 4,726,565 + ABIE41_RS22950 0.14 +0.8 4,726,566 - ABIE41_RS22950 0.14 -1.4 4,726,806 - ABIE41_RS22950 0.29 -2.8 4,727,076 - ABIE41_RS22950 0.45 -1.0 4,727,076 - ABIE41_RS22950 0.45 -1.1 4,727,222 + ABIE41_RS22950 0.54 -4.9 4,727,237 + ABIE41_RS22950 0.55 -3.4 4,727,291 + ABIE41_RS22950 0.58 -4.1 4,727,295 - ABIE41_RS22950 0.59 -0.2 4,727,407 + ABIE41_RS22950 0.65 +0.7 4,727,525 + ABIE41_RS22950 0.73 -3.5 4,727,525 + ABIE41_RS22950 0.73 +0.2 4,727,526 - ABIE41_RS22950 0.73 +0.2 4,727,528 + ABIE41_RS22950 0.73 -0.5 4,727,529 - ABIE41_RS22950 0.73 -0.5 4,727,594 + ABIE41_RS22950 0.77 -1.8 4,727,595 - ABIE41_RS22950 0.77 -1.8 4,727,598 - ABIE41_RS22950 0.77 +1.8 4,727,622 - ABIE41_RS22950 0.79 -1.2 4,727,733 - ABIE41_RS22950 0.85 -0.8 4,727,742 - ABIE41_RS22950 0.86 +0.2 4,727,789 + ABIE41_RS22950 0.89 -2.5 4,727,789 + ABIE41_RS22950 0.89 -2.5 4,727,790 - ABIE41_RS22950 0.89 +0.2 4,727,790 - ABIE41_RS22950 0.89 +0.8 4,727,790 - ABIE41_RS22950 0.89 -2.0 4,727,790 - ABIE41_RS22950 0.89 -0.5 4,727,822 + -1.2 4,727,970 - -1.0 4,728,517 - ABIE41_RS22955 0.43 -2.0 4,728,517 - ABIE41_RS22955 0.43 -1.2 4,728,564 - ABIE41_RS22955 0.48 -2.4 4,728,664 + ABIE41_RS22955 0.58 -1.2 4,728,721 - ABIE41_RS22955 0.64 -1.2 4,728,792 + ABIE41_RS22955 0.72 -0.8
Or see this region's nucleotide sequence