Experiment: R2A with 6-Pentyl-2H-pyran-2-one 0.2865 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS12415 and ABIE41_RS12420 are separated by 108 nucleotides ABIE41_RS12420 and ABIE41_RS12425 are separated by 1 nucleotides ABIE41_RS12425 and ABIE41_RS12430 are separated by 53 nucleotides
ABIE41_RS12415: ABIE41_RS12415 - LysR family transcriptional regulator, at 2,541,985 to 2,542,866
_RS12415
ABIE41_RS12420: ABIE41_RS12420 - acyclic terpene utilization AtuA family protein, at 2,542,975 to 2,544,315
_RS12420
ABIE41_RS12425: ABIE41_RS12425 - hypothetical protein, at 2,544,317 to 2,544,628
_RS12425
ABIE41_RS12430: ABIE41_RS12430 - tripartite tricarboxylate transporter substrate-binding protein, at 2,544,682 to 2,545,638
_RS12430
Position (kb)
2542
2543
2544
2545 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2541.986 kb on + strand at 2541.987 kb on - strand at 2542.212 kb on + strand, within ABIE41_RS12415 at 2542.214 kb on + strand, within ABIE41_RS12415 at 2542.214 kb on + strand, within ABIE41_RS12415 at 2542.214 kb on + strand, within ABIE41_RS12415 at 2542.214 kb on + strand, within ABIE41_RS12415 at 2542.214 kb on + strand, within ABIE41_RS12415 at 2542.214 kb on + strand, within ABIE41_RS12415 at 2542.214 kb on + strand, within ABIE41_RS12415 at 2542.215 kb on - strand, within ABIE41_RS12415 at 2542.215 kb on - strand, within ABIE41_RS12415 at 2542.215 kb on - strand, within ABIE41_RS12415 at 2542.215 kb on - strand, within ABIE41_RS12415 at 2542.215 kb on - strand, within ABIE41_RS12415 at 2542.215 kb on - strand, within ABIE41_RS12415 at 2542.215 kb on - strand, within ABIE41_RS12415 at 2542.215 kb on - strand, within ABIE41_RS12415 at 2542.215 kb on - strand, within ABIE41_RS12415 at 2542.888 kb on - strand at 2542.897 kb on + strand at 2542.929 kb on + strand at 2542.930 kb on - strand at 2543.214 kb on + strand, within ABIE41_RS12420 at 2543.220 kb on + strand, within ABIE41_RS12420 at 2543.220 kb on + strand, within ABIE41_RS12420 at 2543.221 kb on - strand, within ABIE41_RS12420 at 2543.414 kb on - strand, within ABIE41_RS12420 at 2543.414 kb on - strand, within ABIE41_RS12420 at 2543.497 kb on + strand, within ABIE41_RS12420 at 2543.498 kb on - strand, within ABIE41_RS12420 at 2543.498 kb on - strand, within ABIE41_RS12420 at 2543.498 kb on - strand, within ABIE41_RS12420 at 2543.611 kb on + strand, within ABIE41_RS12420 at 2543.612 kb on - strand, within ABIE41_RS12420 at 2543.612 kb on - strand, within ABIE41_RS12420 at 2543.764 kb on + strand, within ABIE41_RS12420 at 2543.764 kb on + strand, within ABIE41_RS12420 at 2543.765 kb on - strand, within ABIE41_RS12420 at 2543.950 kb on + strand, within ABIE41_RS12420 at 2543.950 kb on + strand, within ABIE41_RS12420 at 2544.113 kb on - strand, within ABIE41_RS12420 at 2544.193 kb on + strand at 2544.194 kb on - strand at 2544.485 kb on + strand, within ABIE41_RS12425 at 2544.632 kb on - strand at 2544.717 kb on + strand at 2544.717 kb on + strand at 2544.717 kb on + strand at 2544.718 kb on - strand at 2544.718 kb on - strand at 2544.718 kb on - strand at 2544.718 kb on - strand at 2544.997 kb on + strand, within ABIE41_RS12430 at 2545.106 kb on - strand, within ABIE41_RS12430 at 2545.106 kb on - strand, within ABIE41_RS12430 at 2545.106 kb on - strand, within ABIE41_RS12430 at 2545.207 kb on + strand, within ABIE41_RS12430 at 2545.208 kb on - strand, within ABIE41_RS12430 at 2545.208 kb on - strand, within ABIE41_RS12430 at 2545.208 kb on - strand, within ABIE41_RS12430 at 2545.208 kb on - strand, within ABIE41_RS12430 at 2545.208 kb on - strand, within ABIE41_RS12430 at 2545.216 kb on + strand, within ABIE41_RS12430
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 0.2865 mM remove 2,541,986 + -0.7 2,541,987 - +0.5 2,542,212 + ABIE41_RS12415 0.26 +0.1 2,542,214 + ABIE41_RS12415 0.26 -0.0 2,542,214 + ABIE41_RS12415 0.26 +0.8 2,542,214 + ABIE41_RS12415 0.26 +1.2 2,542,214 + ABIE41_RS12415 0.26 +0.2 2,542,214 + ABIE41_RS12415 0.26 -0.3 2,542,214 + ABIE41_RS12415 0.26 +0.3 2,542,214 + ABIE41_RS12415 0.26 +0.5 2,542,215 - ABIE41_RS12415 0.26 -0.1 2,542,215 - ABIE41_RS12415 0.26 -0.7 2,542,215 - ABIE41_RS12415 0.26 +0.2 2,542,215 - ABIE41_RS12415 0.26 +3.4 2,542,215 - ABIE41_RS12415 0.26 +0.1 2,542,215 - ABIE41_RS12415 0.26 -0.2 2,542,215 - ABIE41_RS12415 0.26 -0.3 2,542,215 - ABIE41_RS12415 0.26 +0.7 2,542,215 - ABIE41_RS12415 0.26 +0.0 2,542,888 - -2.2 2,542,897 + -0.2 2,542,929 + +0.8 2,542,930 - +0.2 2,543,214 + ABIE41_RS12420 0.18 +0.3 2,543,220 + ABIE41_RS12420 0.18 +0.4 2,543,220 + ABIE41_RS12420 0.18 +0.4 2,543,221 - ABIE41_RS12420 0.18 -0.1 2,543,414 - ABIE41_RS12420 0.33 -1.1 2,543,414 - ABIE41_RS12420 0.33 +1.1 2,543,497 + ABIE41_RS12420 0.39 -1.4 2,543,498 - ABIE41_RS12420 0.39 +0.6 2,543,498 - ABIE41_RS12420 0.39 +0.3 2,543,498 - ABIE41_RS12420 0.39 +0.8 2,543,611 + ABIE41_RS12420 0.47 +0.0 2,543,612 - ABIE41_RS12420 0.48 +0.1 2,543,612 - ABIE41_RS12420 0.48 +0.1 2,543,764 + ABIE41_RS12420 0.59 +1.8 2,543,764 + ABIE41_RS12420 0.59 -0.5 2,543,765 - ABIE41_RS12420 0.59 +0.1 2,543,950 + ABIE41_RS12420 0.73 -0.8 2,543,950 + ABIE41_RS12420 0.73 +2.5 2,544,113 - ABIE41_RS12420 0.85 -0.3 2,544,193 + +0.4 2,544,194 - +0.1 2,544,485 + ABIE41_RS12425 0.54 -0.9 2,544,632 - +0.2 2,544,717 + -0.4 2,544,717 + +1.8 2,544,717 + -0.1 2,544,718 - +0.1 2,544,718 - +0.4 2,544,718 - -0.1 2,544,718 - -0.6 2,544,997 + ABIE41_RS12430 0.33 +0.5 2,545,106 - ABIE41_RS12430 0.44 -0.0 2,545,106 - ABIE41_RS12430 0.44 +0.2 2,545,106 - ABIE41_RS12430 0.44 -1.1 2,545,207 + ABIE41_RS12430 0.55 +0.5 2,545,208 - ABIE41_RS12430 0.55 +0.6 2,545,208 - ABIE41_RS12430 0.55 -0.4 2,545,208 - ABIE41_RS12430 0.55 -1.7 2,545,208 - ABIE41_RS12430 0.55 -0.3 2,545,208 - ABIE41_RS12430 0.55 -2.1 2,545,216 + ABIE41_RS12430 0.56 -2.0
Or see this region's nucleotide sequence