Experiment: R2A with 6-Pentyl-2H-pyran-2-one 0.2865 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS12215 and ABIE41_RS12220 are separated by 9 nucleotides ABIE41_RS12220 and ABIE41_RS12225 overlap by 1 nucleotides ABIE41_RS12225 and ABIE41_RS12230 are separated by 70 nucleotides ABIE41_RS12230 and ABIE41_RS12235 are separated by 318 nucleotides ABIE41_RS12235 and ABIE41_RS12240 are separated by 32 nucleotides
ABIE41_RS12215: ABIE41_RS12215 - GntR family transcriptional regulator, at 2,500,453 to 2,501,139
_RS12215
ABIE41_RS12220: ABIE41_RS12220 - PIN domain-containing protein, at 2,501,149 to 2,501,583
_RS12220
ABIE41_RS12225: ABIE41_RS12225 - type II toxin-antitoxin system Phd/YefM family antitoxin, at 2,501,583 to 2,501,825
_RS12225
ABIE41_RS12230: ABIE41_RS12230 - allophanate hydrolase, at 2,501,896 to 2,503,692
_RS12230
ABIE41_RS12235: ABIE41_RS12235 - isochorismatase family cysteine hydrolase, at 2,504,011 to 2,504,649
_RS12235
ABIE41_RS12240: ABIE41_RS12240 - regulator, at 2,504,682 to 2,506,298
_RS12240
Position (kb)
2501
2502
2503
2504 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2500.903 kb on + strand, within ABIE41_RS12215 at 2500.904 kb on - strand, within ABIE41_RS12215 at 2500.904 kb on - strand, within ABIE41_RS12215 at 2500.904 kb on - strand, within ABIE41_RS12215 at 2500.925 kb on - strand, within ABIE41_RS12215 at 2501.014 kb on + strand, within ABIE41_RS12215 at 2501.014 kb on + strand, within ABIE41_RS12215 at 2501.014 kb on + strand, within ABIE41_RS12215 at 2501.015 kb on - strand, within ABIE41_RS12215 at 2501.015 kb on - strand, within ABIE41_RS12215 at 2501.015 kb on - strand, within ABIE41_RS12215 at 2501.122 kb on + strand at 2501.123 kb on - strand at 2501.363 kb on + strand, within ABIE41_RS12220 at 2501.364 kb on - strand, within ABIE41_RS12220 at 2501.380 kb on - strand, within ABIE41_RS12220 at 2501.470 kb on + strand, within ABIE41_RS12220 at 2501.512 kb on + strand, within ABIE41_RS12220 at 2501.512 kb on + strand, within ABIE41_RS12220 at 2501.513 kb on - strand, within ABIE41_RS12220 at 2501.579 kb on + strand at 2501.579 kb on + strand at 2501.580 kb on - strand at 2501.580 kb on - strand at 2501.628 kb on - strand, within ABIE41_RS12225 at 2501.676 kb on + strand, within ABIE41_RS12225 at 2501.696 kb on - strand, within ABIE41_RS12225 at 2501.709 kb on + strand, within ABIE41_RS12225 at 2501.846 kb on - strand at 2501.857 kb on + strand at 2501.858 kb on - strand at 2501.858 kb on - strand at 2502.513 kb on + strand, within ABIE41_RS12230 at 2502.514 kb on - strand, within ABIE41_RS12230 at 2502.514 kb on - strand, within ABIE41_RS12230 at 2502.515 kb on + strand, within ABIE41_RS12230 at 2502.515 kb on + strand, within ABIE41_RS12230 at 2502.516 kb on - strand, within ABIE41_RS12230 at 2502.516 kb on - strand, within ABIE41_RS12230 at 2502.797 kb on + strand, within ABIE41_RS12230 at 2502.798 kb on - strand, within ABIE41_RS12230 at 2502.818 kb on + strand, within ABIE41_RS12230 at 2502.831 kb on + strand, within ABIE41_RS12230 at 2503.616 kb on + strand at 2503.616 kb on + strand at 2503.617 kb on - strand at 2503.617 kb on - strand at 2503.617 kb on - strand at 2504.374 kb on + strand, within ABIE41_RS12235 at 2504.375 kb on - strand, within ABIE41_RS12235 at 2504.375 kb on - strand, within ABIE41_RS12235 at 2504.527 kb on + strand, within ABIE41_RS12235 at 2504.527 kb on + strand, within ABIE41_RS12235 at 2504.528 kb on - strand, within ABIE41_RS12235 at 2504.528 kb on - strand, within ABIE41_RS12235 at 2504.528 kb on - strand, within ABIE41_RS12235 at 2504.528 kb on - strand, within ABIE41_RS12235 at 2504.528 kb on - strand, within ABIE41_RS12235 at 2504.528 kb on - strand, within ABIE41_RS12235
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 0.2865 mM remove 2,500,903 + ABIE41_RS12215 0.66 +0.1 2,500,904 - ABIE41_RS12215 0.66 -0.4 2,500,904 - ABIE41_RS12215 0.66 +0.1 2,500,904 - ABIE41_RS12215 0.66 -0.7 2,500,925 - ABIE41_RS12215 0.69 +1.2 2,501,014 + ABIE41_RS12215 0.82 +1.2 2,501,014 + ABIE41_RS12215 0.82 -0.4 2,501,014 + ABIE41_RS12215 0.82 +0.6 2,501,015 - ABIE41_RS12215 0.82 +0.3 2,501,015 - ABIE41_RS12215 0.82 +1.5 2,501,015 - ABIE41_RS12215 0.82 +0.4 2,501,122 + -0.3 2,501,123 - +0.8 2,501,363 + ABIE41_RS12220 0.49 +1.1 2,501,364 - ABIE41_RS12220 0.49 -0.1 2,501,380 - ABIE41_RS12220 0.53 -1.0 2,501,470 + ABIE41_RS12220 0.74 +0.5 2,501,512 + ABIE41_RS12220 0.83 +0.3 2,501,512 + ABIE41_RS12220 0.83 +0.7 2,501,513 - ABIE41_RS12220 0.84 -0.1 2,501,579 + -1.9 2,501,579 + +0.3 2,501,580 - +0.5 2,501,580 - +0.2 2,501,628 - ABIE41_RS12225 0.19 -0.9 2,501,676 + ABIE41_RS12225 0.38 -0.3 2,501,696 - ABIE41_RS12225 0.47 -0.1 2,501,709 + ABIE41_RS12225 0.52 +1.1 2,501,846 - -0.1 2,501,857 + +0.9 2,501,858 - +0.6 2,501,858 - -0.9 2,502,513 + ABIE41_RS12230 0.34 +0.3 2,502,514 - ABIE41_RS12230 0.34 -0.1 2,502,514 - ABIE41_RS12230 0.34 +0.2 2,502,515 + ABIE41_RS12230 0.34 +0.0 2,502,515 + ABIE41_RS12230 0.34 +0.0 2,502,516 - ABIE41_RS12230 0.35 +0.1 2,502,516 - ABIE41_RS12230 0.35 -0.3 2,502,797 + ABIE41_RS12230 0.50 +0.8 2,502,798 - ABIE41_RS12230 0.50 +2.1 2,502,818 + ABIE41_RS12230 0.51 +0.7 2,502,831 + ABIE41_RS12230 0.52 +0.4 2,503,616 + +0.2 2,503,616 + +0.2 2,503,617 - -0.6 2,503,617 - -0.1 2,503,617 - +0.0 2,504,374 + ABIE41_RS12235 0.57 +0.3 2,504,375 - ABIE41_RS12235 0.57 +1.3 2,504,375 - ABIE41_RS12235 0.57 +1.1 2,504,527 + ABIE41_RS12235 0.81 +0.3 2,504,527 + ABIE41_RS12235 0.81 +0.4 2,504,528 - ABIE41_RS12235 0.81 +0.6 2,504,528 - ABIE41_RS12235 0.81 -0.4 2,504,528 - ABIE41_RS12235 0.81 +0.7 2,504,528 - ABIE41_RS12235 0.81 -1.8 2,504,528 - ABIE41_RS12235 0.81 +0.0 2,504,528 - ABIE41_RS12235 0.81 -0.1
Or see this region's nucleotide sequence