Experiment: R2A with 6-Pentyl-2H-pyran-2-one 0.2865 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS08700 and ABIE41_RS08705 are separated by 48 nucleotides ABIE41_RS08705 and ABIE41_RS08710 are separated by 308 nucleotides ABIE41_RS08710 and ABIE41_RS08715 are separated by 7 nucleotides ABIE41_RS08715 and ABIE41_RS08720 are separated by 35 nucleotides
ABIE41_RS08700: ABIE41_RS08700 - bifunctional diguanylate cyclase/phosphodiesterase, at 1,801,166 to 1,802,860
_RS08700
ABIE41_RS08705: ABIE41_RS08705 - polyhydroxyalkanoate synthesis repressor PhaR, at 1,802,909 to 1,803,505
_RS08705
ABIE41_RS08710: ABIE41_RS08710 - acetyl-CoA C-acetyltransferase, at 1,803,814 to 1,804,992
_RS08710
ABIE41_RS08715: ABIE41_RS08715 - DUF1737 domain-containing protein, at 1,805,000 to 1,805,215
_RS08715
ABIE41_RS08720: ABIE41_RS08720 - acetoacetyl-CoA reductase, at 1,805,251 to 1,805,973
_RS08720
Position (kb)
1803
1804
1805 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1803.372 kb on + strand, within ABIE41_RS08705 at 1803.372 kb on + strand, within ABIE41_RS08705 at 1803.372 kb on + strand, within ABIE41_RS08705 at 1803.429 kb on + strand, within ABIE41_RS08705 at 1803.429 kb on + strand, within ABIE41_RS08705 at 1803.451 kb on - strand at 1803.469 kb on - strand at 1804.280 kb on - strand, within ABIE41_RS08710 at 1805.021 kb on + strand at 1805.021 kb on + strand at 1805.021 kb on + strand at 1805.022 kb on - strand, within ABIE41_RS08715 at 1805.022 kb on - strand, within ABIE41_RS08715 at 1805.027 kb on + strand, within ABIE41_RS08715 at 1805.028 kb on - strand, within ABIE41_RS08715 at 1805.099 kb on + strand, within ABIE41_RS08715 at 1805.183 kb on + strand, within ABIE41_RS08715 at 1805.184 kb on - strand, within ABIE41_RS08715 at 1805.184 kb on - strand, within ABIE41_RS08715 at 1805.213 kb on + strand at 1805.213 kb on + strand at 1805.213 kb on + strand at 1805.213 kb on + strand at 1805.348 kb on - strand, within ABIE41_RS08720 at 1805.348 kb on - strand, within ABIE41_RS08720 at 1805.426 kb on - strand, within ABIE41_RS08720 at 1805.785 kb on + strand, within ABIE41_RS08720 at 1805.785 kb on + strand, within ABIE41_RS08720 at 1805.786 kb on - strand, within ABIE41_RS08720 at 1805.786 kb on - strand, within ABIE41_RS08720 at 1805.786 kb on - strand, within ABIE41_RS08720 at 1805.787 kb on + strand, within ABIE41_RS08720 at 1805.787 kb on + strand, within ABIE41_RS08720 at 1805.962 kb on + strand at 1805.962 kb on + strand at 1805.962 kb on + strand at 1805.962 kb on + strand at 1805.962 kb on + strand at 1805.963 kb on - strand at 1805.963 kb on - strand at 1805.963 kb on - strand at 1805.963 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 0.2865 mM remove 1,803,372 + ABIE41_RS08705 0.78 -1.7 1,803,372 + ABIE41_RS08705 0.78 -2.3 1,803,372 + ABIE41_RS08705 0.78 -0.5 1,803,429 + ABIE41_RS08705 0.87 -0.8 1,803,429 + ABIE41_RS08705 0.87 -1.2 1,803,451 - -1.4 1,803,469 - +0.5 1,804,280 - ABIE41_RS08710 0.40 -0.4 1,805,021 + +1.0 1,805,021 + +0.2 1,805,021 + +2.3 1,805,022 - ABIE41_RS08715 0.10 +0.5 1,805,022 - ABIE41_RS08715 0.10 -0.2 1,805,027 + ABIE41_RS08715 0.12 -1.3 1,805,028 - ABIE41_RS08715 0.13 +0.1 1,805,099 + ABIE41_RS08715 0.46 +0.4 1,805,183 + ABIE41_RS08715 0.85 +0.8 1,805,184 - ABIE41_RS08715 0.85 +0.4 1,805,184 - ABIE41_RS08715 0.85 -0.3 1,805,213 + +1.8 1,805,213 + -0.4 1,805,213 + +1.2 1,805,213 + +0.8 1,805,348 - ABIE41_RS08720 0.13 -0.6 1,805,348 - ABIE41_RS08720 0.13 -0.2 1,805,426 - ABIE41_RS08720 0.24 -0.3 1,805,785 + ABIE41_RS08720 0.74 -0.1 1,805,785 + ABIE41_RS08720 0.74 +0.6 1,805,786 - ABIE41_RS08720 0.74 +0.4 1,805,786 - ABIE41_RS08720 0.74 -0.1 1,805,786 - ABIE41_RS08720 0.74 +0.1 1,805,787 + ABIE41_RS08720 0.74 -0.7 1,805,787 + ABIE41_RS08720 0.74 +0.4 1,805,962 + -0.2 1,805,962 + +0.3 1,805,962 + -1.2 1,805,962 + +1.8 1,805,962 + -1.0 1,805,963 - +0.8 1,805,963 - +0.5 1,805,963 - -0.3 1,805,963 - +0.7
Or see this region's nucleotide sequence