Experiment: R2A with 6-Pentyl-2H-pyran-2-one 0.2865 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS07715 and ABIE41_RS07720 are separated by 61 nucleotides ABIE41_RS07720 and ABIE41_RS07725 are separated by 141 nucleotides ABIE41_RS07725 and ABIE41_RS07730 are separated by 66 nucleotides
ABIE41_RS07715: ABIE41_RS07715 - phosphonate ABC transporter substrate-binding protein, at 1,576,331 to 1,577,248
_RS07715
ABIE41_RS07720: ABIE41_RS07720 - phosphonate ABC transporter ATP-binding protein, at 1,577,310 to 1,578,128
_RS07720
ABIE41_RS07725: ABIE41_RS07725 - TetR/AcrR family transcriptional regulator, at 1,578,270 to 1,578,947
_RS07725
ABIE41_RS07730: ABIE41_RS07730 - efflux RND transporter periplasmic adaptor subunit, at 1,579,014 to 1,580,120
_RS07730
Position (kb) 1577
1578
1579 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1576.392 kb on + strand at 1576.392 kb on + strand at 1576.392 kb on + strand at 1576.393 kb on - strand at 1576.393 kb on - strand at 1576.393 kb on - strand at 1576.393 kb on - strand at 1576.393 kb on - strand at 1576.436 kb on + strand, within ABIE41_RS07715 at 1576.437 kb on + strand, within ABIE41_RS07715 at 1576.437 kb on + strand, within ABIE41_RS07715 at 1576.437 kb on + strand, within ABIE41_RS07715 at 1576.437 kb on + strand, within ABIE41_RS07715 at 1576.437 kb on + strand, within ABIE41_RS07715 at 1576.437 kb on + strand, within ABIE41_RS07715 at 1576.437 kb on + strand, within ABIE41_RS07715 at 1576.438 kb on - strand, within ABIE41_RS07715 at 1576.438 kb on - strand, within ABIE41_RS07715 at 1576.438 kb on - strand, within ABIE41_RS07715 at 1576.438 kb on - strand, within ABIE41_RS07715 at 1576.530 kb on + strand, within ABIE41_RS07715 at 1576.567 kb on - strand, within ABIE41_RS07715 at 1576.567 kb on - strand, within ABIE41_RS07715 at 1576.567 kb on - strand, within ABIE41_RS07715 at 1576.731 kb on + strand, within ABIE41_RS07715 at 1576.731 kb on + strand, within ABIE41_RS07715 at 1576.732 kb on - strand, within ABIE41_RS07715 at 1576.732 kb on - strand, within ABIE41_RS07715 at 1576.733 kb on - strand, within ABIE41_RS07715 at 1576.869 kb on + strand, within ABIE41_RS07715 at 1576.869 kb on + strand, within ABIE41_RS07715 at 1576.869 kb on + strand, within ABIE41_RS07715 at 1576.869 kb on + strand, within ABIE41_RS07715 at 1576.869 kb on + strand, within ABIE41_RS07715 at 1576.869 kb on + strand, within ABIE41_RS07715 at 1576.869 kb on + strand, within ABIE41_RS07715 at 1576.869 kb on + strand, within ABIE41_RS07715 at 1576.869 kb on + strand, within ABIE41_RS07715 at 1576.870 kb on - strand, within ABIE41_RS07715 at 1576.870 kb on - strand, within ABIE41_RS07715 at 1576.870 kb on - strand, within ABIE41_RS07715 at 1576.870 kb on - strand, within ABIE41_RS07715 at 1576.873 kb on - strand, within ABIE41_RS07715 at 1576.881 kb on + strand, within ABIE41_RS07715 at 1576.884 kb on + strand, within ABIE41_RS07715 at 1576.885 kb on - strand, within ABIE41_RS07715 at 1576.885 kb on - strand, within ABIE41_RS07715 at 1576.974 kb on + strand, within ABIE41_RS07715 at 1576.974 kb on + strand, within ABIE41_RS07715 at 1576.974 kb on + strand, within ABIE41_RS07715 at 1576.974 kb on + strand, within ABIE41_RS07715 at 1576.974 kb on + strand, within ABIE41_RS07715 at 1576.974 kb on + strand, within ABIE41_RS07715 at 1576.974 kb on + strand, within ABIE41_RS07715 at 1576.974 kb on + strand, within ABIE41_RS07715 at 1576.974 kb on + strand, within ABIE41_RS07715 at 1576.974 kb on + strand, within ABIE41_RS07715 at 1576.974 kb on + strand, within ABIE41_RS07715 at 1576.974 kb on + strand, within ABIE41_RS07715 at 1576.974 kb on + strand, within ABIE41_RS07715 at 1576.975 kb on - strand, within ABIE41_RS07715 at 1576.975 kb on - strand, within ABIE41_RS07715 at 1576.975 kb on - strand, within ABIE41_RS07715 at 1576.975 kb on - strand, within ABIE41_RS07715 at 1576.975 kb on - strand, within ABIE41_RS07715 at 1577.026 kb on - strand, within ABIE41_RS07715 at 1577.076 kb on + strand, within ABIE41_RS07715 at 1577.076 kb on + strand, within ABIE41_RS07715 at 1577.077 kb on - strand, within ABIE41_RS07715 at 1577.077 kb on - strand, within ABIE41_RS07715 at 1577.077 kb on - strand, within ABIE41_RS07715 at 1577.077 kb on - strand, within ABIE41_RS07715 at 1577.094 kb on + strand, within ABIE41_RS07715 at 1577.094 kb on + strand, within ABIE41_RS07715 at 1577.094 kb on + strand, within ABIE41_RS07715 at 1577.155 kb on - strand, within ABIE41_RS07715 at 1577.817 kb on + strand, within ABIE41_RS07720 at 1578.216 kb on + strand at 1578.216 kb on + strand at 1578.310 kb on + strand at 1578.310 kb on + strand at 1578.310 kb on + strand at 1578.311 kb on - strand at 1578.311 kb on - strand at 1578.311 kb on - strand at 1578.311 kb on - strand at 1578.311 kb on - strand at 1578.311 kb on - strand at 1578.514 kb on + strand, within ABIE41_RS07725 at 1578.742 kb on + strand, within ABIE41_RS07725 at 1578.742 kb on + strand, within ABIE41_RS07725 at 1578.742 kb on + strand, within ABIE41_RS07725 at 1578.742 kb on + strand, within ABIE41_RS07725 at 1578.742 kb on + strand, within ABIE41_RS07725 at 1578.742 kb on + strand, within ABIE41_RS07725 at 1578.743 kb on - strand, within ABIE41_RS07725 at 1578.743 kb on - strand, within ABIE41_RS07725 at 1578.743 kb on - strand, within ABIE41_RS07725 at 1578.743 kb on - strand, within ABIE41_RS07725 at 1578.803 kb on - strand, within ABIE41_RS07725 at 1578.813 kb on + strand, within ABIE41_RS07725 at 1578.814 kb on - strand, within ABIE41_RS07725 at 1578.957 kb on + strand at 1578.984 kb on + strand at 1579.013 kb on + strand at 1579.013 kb on + strand at 1579.013 kb on + strand at 1579.013 kb on + strand at 1579.013 kb on + strand at 1579.013 kb on + strand at 1579.014 kb on - strand at 1579.014 kb on - strand at 1579.014 kb on - strand at 1579.014 kb on - strand at 1579.014 kb on - strand at 1579.029 kb on + strand at 1579.029 kb on + strand at 1579.029 kb on + strand at 1579.066 kb on - strand at 1579.068 kb on + strand at 1579.068 kb on + strand at 1579.068 kb on + strand at 1579.068 kb on + strand at 1579.069 kb on - strand at 1579.069 kb on - strand at 1579.069 kb on - strand at 1579.069 kb on - strand at 1579.069 kb on - strand at 1579.069 kb on - strand at 1579.080 kb on + strand at 1579.080 kb on + strand at 1579.083 kb on + strand at 1579.084 kb on - strand at 1579.084 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 0.2865 mM remove 1,576,392 + +0.9 1,576,392 + -0.7 1,576,392 + +0.2 1,576,393 - +1.3 1,576,393 - -1.1 1,576,393 - +0.6 1,576,393 - +0.0 1,576,393 - -0.2 1,576,436 + ABIE41_RS07715 0.11 +1.1 1,576,437 + ABIE41_RS07715 0.12 -0.6 1,576,437 + ABIE41_RS07715 0.12 +0.6 1,576,437 + ABIE41_RS07715 0.12 -0.2 1,576,437 + ABIE41_RS07715 0.12 +0.2 1,576,437 + ABIE41_RS07715 0.12 +0.4 1,576,437 + ABIE41_RS07715 0.12 +0.5 1,576,437 + ABIE41_RS07715 0.12 -0.8 1,576,438 - ABIE41_RS07715 0.12 -0.7 1,576,438 - ABIE41_RS07715 0.12 +0.5 1,576,438 - ABIE41_RS07715 0.12 -1.3 1,576,438 - ABIE41_RS07715 0.12 +0.5 1,576,530 + ABIE41_RS07715 0.22 +0.1 1,576,567 - ABIE41_RS07715 0.26 +1.9 1,576,567 - ABIE41_RS07715 0.26 -0.0 1,576,567 - ABIE41_RS07715 0.26 -0.2 1,576,731 + ABIE41_RS07715 0.44 -0.9 1,576,731 + ABIE41_RS07715 0.44 +0.1 1,576,732 - ABIE41_RS07715 0.44 +0.5 1,576,732 - ABIE41_RS07715 0.44 +1.5 1,576,733 - ABIE41_RS07715 0.44 +0.9 1,576,869 + ABIE41_RS07715 0.59 +0.2 1,576,869 + ABIE41_RS07715 0.59 +1.5 1,576,869 + ABIE41_RS07715 0.59 +1.8 1,576,869 + ABIE41_RS07715 0.59 +0.1 1,576,869 + ABIE41_RS07715 0.59 +0.3 1,576,869 + ABIE41_RS07715 0.59 +2.1 1,576,869 + ABIE41_RS07715 0.59 -2.5 1,576,869 + ABIE41_RS07715 0.59 +0.2 1,576,869 + ABIE41_RS07715 0.59 +1.9 1,576,870 - ABIE41_RS07715 0.59 +0.6 1,576,870 - ABIE41_RS07715 0.59 +1.0 1,576,870 - ABIE41_RS07715 0.59 +0.4 1,576,870 - ABIE41_RS07715 0.59 +0.2 1,576,873 - ABIE41_RS07715 0.59 -0.1 1,576,881 + ABIE41_RS07715 0.60 -0.2 1,576,884 + ABIE41_RS07715 0.60 -3.5 1,576,885 - ABIE41_RS07715 0.60 -0.6 1,576,885 - ABIE41_RS07715 0.60 -0.1 1,576,974 + ABIE41_RS07715 0.70 -1.1 1,576,974 + ABIE41_RS07715 0.70 +0.1 1,576,974 + ABIE41_RS07715 0.70 +0.7 1,576,974 + ABIE41_RS07715 0.70 -0.2 1,576,974 + ABIE41_RS07715 0.70 +0.5 1,576,974 + ABIE41_RS07715 0.70 +0.1 1,576,974 + ABIE41_RS07715 0.70 +0.5 1,576,974 + ABIE41_RS07715 0.70 -1.3 1,576,974 + ABIE41_RS07715 0.70 +0.1 1,576,974 + ABIE41_RS07715 0.70 -0.3 1,576,974 + ABIE41_RS07715 0.70 +0.9 1,576,974 + ABIE41_RS07715 0.70 +0.1 1,576,974 + ABIE41_RS07715 0.70 -0.5 1,576,975 - ABIE41_RS07715 0.70 +1.7 1,576,975 - ABIE41_RS07715 0.70 -1.7 1,576,975 - ABIE41_RS07715 0.70 +0.5 1,576,975 - ABIE41_RS07715 0.70 +0.3 1,576,975 - ABIE41_RS07715 0.70 +0.8 1,577,026 - ABIE41_RS07715 0.76 +0.1 1,577,076 + ABIE41_RS07715 0.81 -0.6 1,577,076 + ABIE41_RS07715 0.81 -0.4 1,577,077 - ABIE41_RS07715 0.81 +0.9 1,577,077 - ABIE41_RS07715 0.81 +0.7 1,577,077 - ABIE41_RS07715 0.81 +0.1 1,577,077 - ABIE41_RS07715 0.81 -1.6 1,577,094 + ABIE41_RS07715 0.83 -0.5 1,577,094 + ABIE41_RS07715 0.83 -0.5 1,577,094 + ABIE41_RS07715 0.83 -0.0 1,577,155 - ABIE41_RS07715 0.90 +0.3 1,577,817 + ABIE41_RS07720 0.62 -0.1 1,578,216 + -0.0 1,578,216 + +1.3 1,578,310 + -0.9 1,578,310 + +0.5 1,578,310 + -0.3 1,578,311 - +0.2 1,578,311 - +0.2 1,578,311 - -0.4 1,578,311 - -0.5 1,578,311 - -0.5 1,578,311 - -1.5 1,578,514 + ABIE41_RS07725 0.36 -0.5 1,578,742 + ABIE41_RS07725 0.70 +1.1 1,578,742 + ABIE41_RS07725 0.70 -1.8 1,578,742 + ABIE41_RS07725 0.70 +0.7 1,578,742 + ABIE41_RS07725 0.70 -0.3 1,578,742 + ABIE41_RS07725 0.70 +1.0 1,578,742 + ABIE41_RS07725 0.70 -0.2 1,578,743 - ABIE41_RS07725 0.70 +1.9 1,578,743 - ABIE41_RS07725 0.70 +2.1 1,578,743 - ABIE41_RS07725 0.70 -0.6 1,578,743 - ABIE41_RS07725 0.70 -0.2 1,578,803 - ABIE41_RS07725 0.79 +1.9 1,578,813 + ABIE41_RS07725 0.80 +0.1 1,578,814 - ABIE41_RS07725 0.80 +1.1 1,578,957 + -1.1 1,578,984 + -0.2 1,579,013 + -0.5 1,579,013 + -0.4 1,579,013 + +1.7 1,579,013 + +0.3 1,579,013 + -0.1 1,579,013 + +0.1 1,579,014 - -0.5 1,579,014 - +0.8 1,579,014 - +0.4 1,579,014 - -0.8 1,579,014 - +0.6 1,579,029 + -0.3 1,579,029 + -0.0 1,579,029 + -0.5 1,579,066 - +2.9 1,579,068 + -0.4 1,579,068 + +0.3 1,579,068 + +0.1 1,579,068 + -0.5 1,579,069 - +0.2 1,579,069 - -0.7 1,579,069 - +0.8 1,579,069 - -0.3 1,579,069 - +0.2 1,579,069 - +0.6 1,579,080 + -0.1 1,579,080 + +0.1 1,579,083 + -0.3 1,579,084 - -0.1 1,579,084 - -0.3
Or see this region's nucleotide sequence