Strain Fitness in Escherichia coli BL21 around ECD_00592

Experiment: Bas49

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00591 and ECD_00592 are separated by 587 nucleotidesECD_00592 and ECD_00593 are separated by 168 nucleotidesECD_00593 and ECD_00594 are separated by 54 nucleotidesECD_00594 and ECD_00595 are separated by 92 nucleotides ECD_00591: ECD_00591 - anaerobic C4-dicarboxylate transport, at 614,950 to 616,335 _00591 ECD_00592: ECD_00592 - phospholipid:lipid A palmitoyltransferase, at 616,923 to 617,483 _00592 ECD_00593: ECD_00593 - constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA-binding protein, at 617,652 to 617,861 _00593 ECD_00594: ECD_00594 - fluoride efflux channel, dual topology membrane protein, at 617,916 to 618,299 _00594 ECD_00595: ECD_00595 - putative NAD(P)-binding amidase-type enzyme YbeM (C-N hydrolase family), at 618,392 to 619,180 _00595 Position (kb) 616 617 618Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 615.997 kb on - strand, within ECD_00591at 615.999 kb on + strand, within ECD_00591at 616.000 kb on - strand, within ECD_00591at 616.036 kb on - strand, within ECD_00591at 616.062 kb on - strand, within ECD_00591at 616.243 kb on + strandat 616.244 kb on - strandat 616.286 kb on - strandat 616.881 kb on + strandat 617.206 kb on - strand, within ECD_00592at 617.206 kb on - strand, within ECD_00592at 617.421 kb on + strand, within ECD_00592at 617.422 kb on - strand, within ECD_00592at 617.437 kb on - strandat 617.437 kb on - strandat 617.627 kb on + strandat 617.638 kb on + strandat 617.639 kb on - strandat 617.639 kb on - strandat 617.675 kb on + strand, within ECD_00593at 617.675 kb on + strand, within ECD_00593at 617.675 kb on + strand, within ECD_00593at 617.774 kb on + strand, within ECD_00593at 617.849 kb on - strandat 617.860 kb on - strandat 617.918 kb on - strandat 618.028 kb on - strand, within ECD_00594at 618.157 kb on - strand, within ECD_00594at 618.157 kb on - strand, within ECD_00594at 618.295 kb on - strandat 618.339 kb on + strandat 618.350 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas49
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615,997 - ECD_00591 0.76 -0.5
615,999 + ECD_00591 0.76 +0.5
616,000 - ECD_00591 0.76 -1.7
616,036 - ECD_00591 0.78 +0.4
616,062 - ECD_00591 0.80 +1.2
616,243 + -0.1
616,244 - -1.1
616,286 - -0.2
616,881 + +2.0
617,206 - ECD_00592 0.50 +2.1
617,206 - ECD_00592 0.50 -0.5
617,421 + ECD_00592 0.89 +0.6
617,422 - ECD_00592 0.89 -1.3
617,437 - -1.3
617,437 - -0.2
617,627 + +0.4
617,638 + -0.1
617,639 - +0.9
617,639 - +1.5
617,675 + ECD_00593 0.11 +1.9
617,675 + ECD_00593 0.11 +0.8
617,675 + ECD_00593 0.11 -2.6
617,774 + ECD_00593 0.58 -1.0
617,849 - -1.3
617,860 - +0.8
617,918 - -0.8
618,028 - ECD_00594 0.29 -2.7
618,157 - ECD_00594 0.63 -2.2
618,157 - ECD_00594 0.63 -0.4
618,295 - +0.9
618,339 + +1.0
618,350 - -3.1

Or see this region's nucleotide sequence