Experiment: HBM-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS25330 and LU632_RS25335 overlap by 59 nucleotides LU632_RS25335 and LU632_RS25340 are separated by 7 nucleotides LU632_RS25340 and LU632_RS25345 are separated by 100 nucleotides
LU632_RS25330: LU632_RS25330 - DUF5710 domain-containing protein, at 214 to 840
_RS25330
LU632_RS25335: LU632_RS25335 - hypothetical protein, at 782 to 3,604
_RS25335
LU632_RS25340: LU632_RS25340 - hypothetical protein, at 3,612 to 4,055
_RS25340
LU632_RS25345: LU632_RS25345 - DotI/IcmL family type IV secretion protein, at 4,156 to 4,776
_RS25345
Position (kb)
0
1
2
3
4 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 0.045 kb on + strand at 0.072 kb on + strand at 0.209 kb on + strand at 0.209 kb on + strand at 0.209 kb on + strand at 0.210 kb on - strand at 0.210 kb on - strand at 0.210 kb on - strand at 0.213 kb on + strand at 0.226 kb on - strand at 0.226 kb on - strand at 0.230 kb on - strand at 0.238 kb on - strand at 0.238 kb on - strand at 0.298 kb on + strand, within LU632_RS25330 at 0.298 kb on + strand, within LU632_RS25330 at 0.298 kb on + strand, within LU632_RS25330 at 0.298 kb on + strand, within LU632_RS25330 at 0.299 kb on - strand, within LU632_RS25330 at 0.299 kb on - strand, within LU632_RS25330 at 0.299 kb on - strand, within LU632_RS25330 at 0.299 kb on - strand, within LU632_RS25330 at 0.299 kb on - strand, within LU632_RS25330 at 0.299 kb on - strand, within LU632_RS25330 at 0.310 kb on + strand, within LU632_RS25330 at 0.560 kb on + strand, within LU632_RS25330 at 0.560 kb on + strand, within LU632_RS25330 at 0.560 kb on + strand, within LU632_RS25330 at 0.560 kb on + strand, within LU632_RS25330 at 0.561 kb on - strand, within LU632_RS25330 at 0.561 kb on - strand, within LU632_RS25330 at 0.561 kb on - strand, within LU632_RS25330 at 0.562 kb on + strand, within LU632_RS25330 at 0.562 kb on + strand, within LU632_RS25330 at 0.598 kb on + strand, within LU632_RS25330 at 0.658 kb on + strand, within LU632_RS25330 at 0.658 kb on + strand, within LU632_RS25330 at 0.658 kb on + strand, within LU632_RS25330 at 0.659 kb on - strand, within LU632_RS25330 at 0.659 kb on - strand, within LU632_RS25330 at 0.659 kb on - strand, within LU632_RS25330 at 0.722 kb on + strand, within LU632_RS25330 at 0.722 kb on + strand, within LU632_RS25330 at 0.723 kb on - strand, within LU632_RS25330 at 0.723 kb on - strand, within LU632_RS25330 at 0.723 kb on - strand, within LU632_RS25330 at 0.809 kb on + strand at 0.810 kb on - strand at 0.857 kb on - strand at 0.861 kb on - strand at 0.861 kb on - strand at 0.861 kb on - strand at 0.883 kb on - strand at 0.883 kb on - strand at 0.887 kb on - strand at 0.887 kb on - strand at 0.917 kb on + strand at 0.917 kb on + strand at 0.918 kb on - strand at 0.918 kb on - strand at 1.032 kb on - strand at 1.050 kb on + strand at 1.051 kb on - strand at 1.256 kb on + strand, within LU632_RS25335 at 1.257 kb on - strand, within LU632_RS25335 at 1.334 kb on - strand, within LU632_RS25335 at 1.420 kb on + strand, within LU632_RS25335 at 1.517 kb on - strand, within LU632_RS25335 at 1.546 kb on + strand, within LU632_RS25335 at 1.546 kb on + strand, within LU632_RS25335 at 1.547 kb on - strand, within LU632_RS25335 at 1.547 kb on - strand, within LU632_RS25335 at 1.549 kb on - strand, within LU632_RS25335 at 1.549 kb on - strand, within LU632_RS25335 at 1.549 kb on - strand, within LU632_RS25335 at 1.668 kb on + strand, within LU632_RS25335 at 1.668 kb on + strand, within LU632_RS25335 at 1.668 kb on + strand, within LU632_RS25335 at 1.668 kb on + strand, within LU632_RS25335 at 1.668 kb on + strand, within LU632_RS25335 at 1.669 kb on - strand, within LU632_RS25335 at 1.669 kb on - strand, within LU632_RS25335 at 1.669 kb on - strand, within LU632_RS25335 at 1.669 kb on - strand, within LU632_RS25335 at 1.716 kb on + strand, within LU632_RS25335 at 1.858 kb on - strand, within LU632_RS25335 at 1.858 kb on - strand, within LU632_RS25335 at 2.105 kb on - strand, within LU632_RS25335 at 2.162 kb on + strand, within LU632_RS25335 at 2.162 kb on + strand, within LU632_RS25335 at 2.163 kb on - strand, within LU632_RS25335 at 2.364 kb on + strand, within LU632_RS25335 at 2.364 kb on + strand, within LU632_RS25335 at 2.365 kb on - strand, within LU632_RS25335 at 2.365 kb on - strand, within LU632_RS25335 at 2.365 kb on - strand, within LU632_RS25335 at 2.494 kb on - strand, within LU632_RS25335 at 2.494 kb on - strand, within LU632_RS25335 at 2.494 kb on - strand, within LU632_RS25335 at 2.497 kb on + strand, within LU632_RS25335 at 2.498 kb on - strand, within LU632_RS25335 at 2.643 kb on + strand, within LU632_RS25335 at 2.643 kb on + strand, within LU632_RS25335 at 2.667 kb on - strand, within LU632_RS25335 at 2.699 kb on + strand, within LU632_RS25335 at 2.699 kb on + strand, within LU632_RS25335 at 2.699 kb on + strand, within LU632_RS25335 at 2.700 kb on - strand, within LU632_RS25335 at 2.807 kb on + strand, within LU632_RS25335 at 2.807 kb on + strand, within LU632_RS25335 at 2.808 kb on - strand, within LU632_RS25335 at 2.808 kb on - strand, within LU632_RS25335 at 2.830 kb on + strand, within LU632_RS25335 at 2.830 kb on + strand, within LU632_RS25335 at 2.831 kb on - strand, within LU632_RS25335 at 2.831 kb on - strand, within LU632_RS25335 at 2.831 kb on - strand, within LU632_RS25335 at 2.873 kb on + strand, within LU632_RS25335 at 2.982 kb on - strand, within LU632_RS25335 at 2.982 kb on - strand, within LU632_RS25335 at 2.982 kb on - strand, within LU632_RS25335 at 2.982 kb on - strand, within LU632_RS25335 at 3.078 kb on - strand, within LU632_RS25335 at 3.078 kb on - strand, within LU632_RS25335 at 3.080 kb on - strand, within LU632_RS25335 at 3.276 kb on + strand, within LU632_RS25335 at 3.617 kb on + strand at 3.708 kb on - strand, within LU632_RS25340 at 3.721 kb on - strand, within LU632_RS25340 at 3.804 kb on - strand, within LU632_RS25340 at 3.906 kb on + strand, within LU632_RS25340 at 3.907 kb on - strand, within LU632_RS25340 at 3.907 kb on - strand, within LU632_RS25340 at 3.940 kb on - strand, within LU632_RS25340 at 4.023 kb on - strand at 4.038 kb on + strand at 4.038 kb on + strand at 4.038 kb on + strand at 4.038 kb on + strand at 4.038 kb on + strand at 4.039 kb on - strand at 4.039 kb on - strand at 4.039 kb on - strand at 4.039 kb on - strand at 4.135 kb on + strand at 4.136 kb on - strand at 4.136 kb on - strand at 4.200 kb on + strand at 4.300 kb on + strand, within LU632_RS25345 at 4.443 kb on - strand, within LU632_RS25345 at 4.443 kb on - strand, within LU632_RS25345 at 4.487 kb on - strand, within LU632_RS25345 at 4.487 kb on - strand, within LU632_RS25345 at 4.489 kb on + strand, within LU632_RS25345 at 4.490 kb on - strand, within LU632_RS25345 at 4.490 kb on - strand, within LU632_RS25345 at 4.490 kb on - strand, within LU632_RS25345
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Glucose remove 45 + +0.7 72 + -0.3 209 + +0.4 209 + +0.2 209 + +0.3 210 - +0.2 210 - +1.2 210 - -0.3 213 + +0.3 226 - -0.8 226 - +0.4 230 - -1.3 238 - +0.3 238 - +0.2 298 + LU632_RS25330 0.13 +0.2 298 + LU632_RS25330 0.13 -0.6 298 + LU632_RS25330 0.13 +1.8 298 + LU632_RS25330 0.13 -0.9 299 - LU632_RS25330 0.14 -0.1 299 - LU632_RS25330 0.14 -0.0 299 - LU632_RS25330 0.14 -0.1 299 - LU632_RS25330 0.14 +0.0 299 - LU632_RS25330 0.14 -0.2 299 - LU632_RS25330 0.14 +0.3 310 + LU632_RS25330 0.15 +0.4 560 + LU632_RS25330 0.55 +0.3 560 + LU632_RS25330 0.55 +0.7 560 + LU632_RS25330 0.55 +1.0 560 + LU632_RS25330 0.55 +1.8 561 - LU632_RS25330 0.55 -0.1 561 - LU632_RS25330 0.55 -0.2 561 - LU632_RS25330 0.55 +0.2 562 + LU632_RS25330 0.56 +0.7 562 + LU632_RS25330 0.56 +0.2 598 + LU632_RS25330 0.61 +0.3 658 + LU632_RS25330 0.71 -0.1 658 + LU632_RS25330 0.71 -0.2 658 + LU632_RS25330 0.71 -0.6 659 - LU632_RS25330 0.71 +0.4 659 - LU632_RS25330 0.71 +0.5 659 - LU632_RS25330 0.71 +0.1 722 + LU632_RS25330 0.81 +0.2 722 + LU632_RS25330 0.81 +0.0 723 - LU632_RS25330 0.81 -0.7 723 - LU632_RS25330 0.81 +0.2 723 - LU632_RS25330 0.81 +0.3 809 + +0.6 810 - +1.0 857 - +0.8 861 - -0.5 861 - -0.5 861 - -1.4 883 - +0.2 883 - +0.4 887 - +1.4 887 - -0.1 917 + +0.3 917 + +0.0 918 - -0.9 918 - +0.2 1,032 - -0.6 1,050 + +0.1 1,051 - -0.1 1,256 + LU632_RS25335 0.17 +0.1 1,257 - LU632_RS25335 0.17 -0.4 1,334 - LU632_RS25335 0.20 -0.6 1,420 + LU632_RS25335 0.23 -0.1 1,517 - LU632_RS25335 0.26 -0.1 1,546 + LU632_RS25335 0.27 -0.2 1,546 + LU632_RS25335 0.27 +0.4 1,547 - LU632_RS25335 0.27 -0.5 1,547 - LU632_RS25335 0.27 +1.1 1,549 - LU632_RS25335 0.27 -1.1 1,549 - LU632_RS25335 0.27 -0.2 1,549 - LU632_RS25335 0.27 +0.4 1,668 + LU632_RS25335 0.31 -0.1 1,668 + LU632_RS25335 0.31 -0.8 1,668 + LU632_RS25335 0.31 +2.4 1,668 + LU632_RS25335 0.31 -1.4 1,668 + LU632_RS25335 0.31 +0.8 1,669 - LU632_RS25335 0.31 +0.3 1,669 - LU632_RS25335 0.31 -0.3 1,669 - LU632_RS25335 0.31 -1.3 1,669 - LU632_RS25335 0.31 +0.4 1,716 + LU632_RS25335 0.33 +1.2 1,858 - LU632_RS25335 0.38 -0.7 1,858 - LU632_RS25335 0.38 -0.6 2,105 - LU632_RS25335 0.47 -1.1 2,162 + LU632_RS25335 0.49 +0.7 2,162 + LU632_RS25335 0.49 +1.0 2,163 - LU632_RS25335 0.49 +0.4 2,364 + LU632_RS25335 0.56 -0.1 2,364 + LU632_RS25335 0.56 +0.9 2,365 - LU632_RS25335 0.56 +1.4 2,365 - LU632_RS25335 0.56 +0.7 2,365 - LU632_RS25335 0.56 -0.0 2,494 - LU632_RS25335 0.61 -1.6 2,494 - LU632_RS25335 0.61 +0.4 2,494 - LU632_RS25335 0.61 +0.3 2,497 + LU632_RS25335 0.61 +1.0 2,498 - LU632_RS25335 0.61 +0.9 2,643 + LU632_RS25335 0.66 -0.0 2,643 + LU632_RS25335 0.66 +0.2 2,667 - LU632_RS25335 0.67 +1.2 2,699 + LU632_RS25335 0.68 -0.1 2,699 + LU632_RS25335 0.68 +0.9 2,699 + LU632_RS25335 0.68 +0.4 2,700 - LU632_RS25335 0.68 +0.1 2,807 + LU632_RS25335 0.72 -0.3 2,807 + LU632_RS25335 0.72 +0.4 2,808 - LU632_RS25335 0.72 -0.0 2,808 - LU632_RS25335 0.72 -3.6 2,830 + LU632_RS25335 0.73 -0.9 2,830 + LU632_RS25335 0.73 -2.6 2,831 - LU632_RS25335 0.73 -0.8 2,831 - LU632_RS25335 0.73 -0.1 2,831 - LU632_RS25335 0.73 +0.1 2,873 + LU632_RS25335 0.74 +0.4 2,982 - LU632_RS25335 0.78 -1.9 2,982 - LU632_RS25335 0.78 +0.6 2,982 - LU632_RS25335 0.78 -0.4 2,982 - LU632_RS25335 0.78 -0.3 3,078 - LU632_RS25335 0.81 +0.4 3,078 - LU632_RS25335 0.81 -1.1 3,080 - LU632_RS25335 0.81 +0.1 3,276 + LU632_RS25335 0.88 -0.2 3,617 + -0.4 3,708 - LU632_RS25340 0.22 -0.1 3,721 - LU632_RS25340 0.25 -1.5 3,804 - LU632_RS25340 0.43 +0.0 3,906 + LU632_RS25340 0.66 -0.6 3,907 - LU632_RS25340 0.66 +0.7 3,907 - LU632_RS25340 0.66 -0.0 3,940 - LU632_RS25340 0.74 -0.1 4,023 - +0.4 4,038 + -0.6 4,038 + -0.1 4,038 + +1.2 4,038 + -2.4 4,038 + -0.3 4,039 - -0.6 4,039 - +0.1 4,039 - +0.1 4,039 - +0.0 4,135 + +3.0 4,136 - -0.3 4,136 - -0.5 4,200 + -0.4 4,300 + LU632_RS25345 0.23 +1.2 4,443 - LU632_RS25345 0.46 +0.5 4,443 - LU632_RS25345 0.46 +0.7 4,487 - LU632_RS25345 0.53 -0.4 4,487 - LU632_RS25345 0.53 +0.2 4,489 + LU632_RS25345 0.54 -0.7 4,490 - LU632_RS25345 0.54 -0.9 4,490 - LU632_RS25345 0.54 +0.1 4,490 - LU632_RS25345 0.54 +0.6
Or see this region's nucleotide sequence