Experiment: HBM-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS22355 and LU632_RS22360 are separated by 79 nucleotides LU632_RS22360 and pgi are separated by 541 nucleotides pgi and LU632_RS22370 are separated by 164 nucleotides LU632_RS22370 and lysC are separated by 563 nucleotides
LU632_RS22355: LU632_RS22355 - YjbF family lipoprotein, at 4,269,577 to 4,270,224
_RS22355
LU632_RS22360: LU632_RS22360 - exopolysaccharide production protein YjbE, at 4,270,304 to 4,270,561
_RS22360
LU632_RS22365: pgi - glucose-6-phosphate isomerase, at 4,271,103 to 4,272,752
pgi
LU632_RS22370: LU632_RS22370 - hypothetical protein, at 4,272,917 to 4,273,075
_RS22370
LU632_RS22375: lysC - lysine-sensitive aspartokinase 3, at 4,273,639 to 4,274,994
lysC
Position (kb) 4271
4272
4273 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 4270.108 kb on + strand, within LU632_RS22355 at 4270.156 kb on - strand, within LU632_RS22355 at 4270.198 kb on + strand at 4270.198 kb on + strand at 4270.199 kb on - strand at 4270.214 kb on - strand at 4270.214 kb on - strand at 4270.215 kb on + strand at 4270.217 kb on + strand at 4270.217 kb on + strand at 4270.218 kb on - strand at 4270.218 kb on - strand at 4270.257 kb on + strand at 4270.285 kb on + strand at 4270.291 kb on + strand at 4270.296 kb on + strand at 4270.296 kb on + strand at 4270.524 kb on + strand, within LU632_RS22360 at 4270.524 kb on + strand, within LU632_RS22360 at 4270.525 kb on - strand, within LU632_RS22360 at 4270.527 kb on - strand, within LU632_RS22360 at 4270.717 kb on - strand at 4271.009 kb on - strand at 4271.009 kb on - strand at 4271.249 kb on + strand at 4271.250 kb on - strand at 4271.287 kb on + strand, within pgi at 4271.385 kb on - strand, within pgi at 4271.580 kb on - strand, within pgi at 4271.674 kb on + strand, within pgi at 4271.674 kb on + strand, within pgi at 4271.693 kb on + strand, within pgi at 4271.711 kb on - strand, within pgi at 4271.731 kb on + strand, within pgi at 4271.731 kb on + strand, within pgi at 4271.731 kb on + strand, within pgi at 4271.741 kb on - strand, within pgi at 4271.750 kb on + strand, within pgi at 4271.750 kb on + strand, within pgi at 4271.783 kb on - strand, within pgi at 4272.021 kb on - strand, within pgi at 4272.122 kb on + strand, within pgi at 4272.207 kb on - strand, within pgi at 4272.236 kb on + strand, within pgi at 4272.263 kb on + strand, within pgi at 4272.263 kb on + strand, within pgi at 4272.264 kb on - strand, within pgi at 4272.328 kb on + strand, within pgi at 4272.329 kb on - strand, within pgi at 4272.435 kb on - strand, within pgi at 4272.438 kb on - strand, within pgi at 4272.783 kb on + strand at 4272.800 kb on + strand at 4272.800 kb on + strand at 4272.800 kb on + strand at 4272.801 kb on - strand at 4272.801 kb on - strand at 4272.801 kb on - strand at 4272.911 kb on - strand at 4273.051 kb on - strand, within LU632_RS22370 at 4273.184 kb on - strand at 4273.189 kb on - strand at 4273.253 kb on + strand at 4273.253 kb on + strand at 4273.268 kb on - strand at 4273.380 kb on + strand at 4273.466 kb on + strand at 4273.552 kb on - strand at 4273.613 kb on + strand at 4273.629 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Glucose remove 4,270,108 + LU632_RS22355 0.82 -0.9 4,270,156 - LU632_RS22355 0.89 -0.1 4,270,198 + -0.1 4,270,198 + -0.6 4,270,199 - +1.1 4,270,214 - +0.3 4,270,214 - -1.5 4,270,215 + -1.7 4,270,217 + -0.3 4,270,217 + -0.2 4,270,218 - +0.7 4,270,218 - -0.6 4,270,257 + -0.2 4,270,285 + -0.4 4,270,291 + +0.1 4,270,296 + +0.6 4,270,296 + -0.0 4,270,524 + LU632_RS22360 0.85 -0.1 4,270,524 + LU632_RS22360 0.85 +0.5 4,270,525 - LU632_RS22360 0.86 +0.2 4,270,527 - LU632_RS22360 0.86 -0.1 4,270,717 - -0.3 4,271,009 - -0.0 4,271,009 - +1.3 4,271,249 + -3.1 4,271,250 - -1.7 4,271,287 + pgi LU632_RS22365 0.11 -0.5 4,271,385 - pgi LU632_RS22365 0.17 -4.5 4,271,580 - pgi LU632_RS22365 0.29 -4.1 4,271,674 + pgi LU632_RS22365 0.35 -4.5 4,271,674 + pgi LU632_RS22365 0.35 -3.7 4,271,693 + pgi LU632_RS22365 0.36 -4.3 4,271,711 - pgi LU632_RS22365 0.37 -0.3 4,271,731 + pgi LU632_RS22365 0.38 -2.1 4,271,731 + pgi LU632_RS22365 0.38 -1.3 4,271,731 + pgi LU632_RS22365 0.38 -2.3 4,271,741 - pgi LU632_RS22365 0.39 -2.1 4,271,750 + pgi LU632_RS22365 0.39 -0.9 4,271,750 + pgi LU632_RS22365 0.39 -2.5 4,271,783 - pgi LU632_RS22365 0.41 -3.3 4,272,021 - pgi LU632_RS22365 0.56 -1.5 4,272,122 + pgi LU632_RS22365 0.62 -1.9 4,272,207 - pgi LU632_RS22365 0.67 -3.6 4,272,236 + pgi LU632_RS22365 0.69 -3.4 4,272,263 + pgi LU632_RS22365 0.70 -2.7 4,272,263 + pgi LU632_RS22365 0.70 -0.9 4,272,264 - pgi LU632_RS22365 0.70 -3.0 4,272,328 + pgi LU632_RS22365 0.74 -4.1 4,272,329 - pgi LU632_RS22365 0.74 -2.8 4,272,435 - pgi LU632_RS22365 0.81 -4.4 4,272,438 - pgi LU632_RS22365 0.81 -2.6 4,272,783 + -2.1 4,272,800 + -0.4 4,272,800 + -0.7 4,272,800 + +0.6 4,272,801 - +0.3 4,272,801 - -0.3 4,272,801 - -0.2 4,272,911 - -0.7 4,273,051 - LU632_RS22370 0.84 +0.4 4,273,184 - +0.1 4,273,189 - -0.5 4,273,253 + -1.5 4,273,253 + +0.4 4,273,268 - -0.8 4,273,380 + -0.3 4,273,466 + -0.1 4,273,552 - +0.7 4,273,613 + -0.4 4,273,629 - +1.1
Or see this region's nucleotide sequence