Experiment: HBM-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyrE and rph are separated by 54 nucleotides rph and LU632_RS22025 are separated by 56 nucleotides LU632_RS22025 and LU632_RS22030 are separated by 134 nucleotides
LU632_RS22015: pyrE - orotate phosphoribosyltransferase, at 4,203,615 to 4,204,256
pyrE
LU632_RS22020: rph - ribonuclease PH, at 4,204,311 to 4,205,027
rph
LU632_RS22025: LU632_RS22025 - hypothetical protein, at 4,205,084 to 4,205,326
_RS22025
LU632_RS22030: LU632_RS22030 - ABC transporter permease, at 4,205,461 to 4,206,552
_RS22030
Position (kb)
4205
4206 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4204.312 kb on + strand at 4204.312 kb on + strand at 4204.578 kb on + strand, within rph at 4204.579 kb on - strand, within rph at 4204.579 kb on - strand, within rph at 4204.583 kb on + strand, within rph at 4204.584 kb on - strand, within rph at 4204.584 kb on - strand, within rph at 4204.694 kb on + strand, within rph at 4204.694 kb on + strand, within rph at 4204.694 kb on + strand, within rph at 4204.695 kb on - strand, within rph at 4204.835 kb on + strand, within rph at 4204.835 kb on + strand, within rph at 4204.836 kb on - strand, within rph at 4204.836 kb on - strand, within rph at 4204.836 kb on - strand, within rph at 4204.836 kb on - strand, within rph at 4204.836 kb on - strand, within rph at 4204.836 kb on - strand, within rph at 4204.841 kb on - strand, within rph at 4204.969 kb on + strand at 4205.054 kb on + strand at 4205.070 kb on + strand at 4205.101 kb on + strand at 4205.102 kb on - strand at 4205.104 kb on + strand at 4205.104 kb on + strand at 4205.104 kb on + strand at 4205.160 kb on + strand, within LU632_RS22025 at 4205.161 kb on - strand, within LU632_RS22025 at 4205.161 kb on - strand, within LU632_RS22025 at 4205.161 kb on - strand, within LU632_RS22025 at 4205.253 kb on - strand, within LU632_RS22025 at 4205.327 kb on - strand at 4205.373 kb on - strand at 4205.396 kb on + strand at 4205.397 kb on - strand at 4205.397 kb on - strand at 4205.399 kb on - strand at 4205.399 kb on - strand at 4205.399 kb on - strand at 4205.399 kb on - strand at 4205.425 kb on - strand at 4205.495 kb on - strand at 4205.495 kb on - strand at 4205.496 kb on + strand at 4205.497 kb on - strand at 4205.612 kb on - strand, within LU632_RS22030 at 4205.662 kb on - strand, within LU632_RS22030 at 4205.674 kb on + strand, within LU632_RS22030 at 4205.675 kb on - strand, within LU632_RS22030 at 4205.691 kb on + strand, within LU632_RS22030 at 4205.723 kb on - strand, within LU632_RS22030 at 4205.754 kb on + strand, within LU632_RS22030 at 4205.780 kb on + strand, within LU632_RS22030 at 4205.794 kb on + strand, within LU632_RS22030 at 4205.795 kb on - strand, within LU632_RS22030 at 4205.796 kb on + strand, within LU632_RS22030 at 4205.796 kb on + strand, within LU632_RS22030 at 4205.796 kb on + strand, within LU632_RS22030 at 4205.797 kb on - strand, within LU632_RS22030 at 4205.859 kb on + strand, within LU632_RS22030 at 4205.876 kb on - strand, within LU632_RS22030 at 4205.891 kb on + strand, within LU632_RS22030 at 4205.892 kb on - strand, within LU632_RS22030 at 4206.050 kb on + strand, within LU632_RS22030 at 4206.058 kb on - strand, within LU632_RS22030 at 4206.087 kb on + strand, within LU632_RS22030 at 4206.289 kb on + strand, within LU632_RS22030 at 4206.290 kb on - strand, within LU632_RS22030 at 4206.306 kb on + strand, within LU632_RS22030 at 4206.307 kb on - strand, within LU632_RS22030
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Glucose remove 4,204,312 + -3.5 4,204,312 + -1.7 4,204,578 + rph LU632_RS22020 0.37 -2.0 4,204,579 - rph LU632_RS22020 0.37 -0.4 4,204,579 - rph LU632_RS22020 0.37 +0.7 4,204,583 + rph LU632_RS22020 0.38 -2.4 4,204,584 - rph LU632_RS22020 0.38 +0.6 4,204,584 - rph LU632_RS22020 0.38 -1.5 4,204,694 + rph LU632_RS22020 0.53 -1.7 4,204,694 + rph LU632_RS22020 0.53 -3.4 4,204,694 + rph LU632_RS22020 0.53 -0.5 4,204,695 - rph LU632_RS22020 0.54 -0.3 4,204,835 + rph LU632_RS22020 0.73 -0.5 4,204,835 + rph LU632_RS22020 0.73 -3.1 4,204,836 - rph LU632_RS22020 0.73 +0.2 4,204,836 - rph LU632_RS22020 0.73 -0.1 4,204,836 - rph LU632_RS22020 0.73 +0.3 4,204,836 - rph LU632_RS22020 0.73 +1.3 4,204,836 - rph LU632_RS22020 0.73 +2.1 4,204,836 - rph LU632_RS22020 0.73 -0.4 4,204,841 - rph LU632_RS22020 0.74 +0.4 4,204,969 + -1.7 4,205,054 + -0.3 4,205,070 + +0.2 4,205,101 + -0.8 4,205,102 - -0.0 4,205,104 + +0.2 4,205,104 + +1.4 4,205,104 + +0.3 4,205,160 + LU632_RS22025 0.31 -0.6 4,205,161 - LU632_RS22025 0.32 -0.9 4,205,161 - LU632_RS22025 0.32 -0.0 4,205,161 - LU632_RS22025 0.32 -0.7 4,205,253 - LU632_RS22025 0.70 +0.7 4,205,327 - +1.1 4,205,373 - +0.8 4,205,396 + +0.0 4,205,397 - +0.0 4,205,397 - +0.8 4,205,399 - +0.2 4,205,399 - -0.1 4,205,399 - +0.1 4,205,399 - +0.3 4,205,425 - +0.6 4,205,495 - -0.1 4,205,495 - +0.2 4,205,496 + +0.4 4,205,497 - -0.0 4,205,612 - LU632_RS22030 0.14 -0.7 4,205,662 - LU632_RS22030 0.18 -0.4 4,205,674 + LU632_RS22030 0.20 +0.1 4,205,675 - LU632_RS22030 0.20 -0.3 4,205,691 + LU632_RS22030 0.21 +0.0 4,205,723 - LU632_RS22030 0.24 +0.4 4,205,754 + LU632_RS22030 0.27 -0.0 4,205,780 + LU632_RS22030 0.29 -0.6 4,205,794 + LU632_RS22030 0.30 -0.4 4,205,795 - LU632_RS22030 0.31 -0.4 4,205,796 + LU632_RS22030 0.31 +0.5 4,205,796 + LU632_RS22030 0.31 +0.1 4,205,796 + LU632_RS22030 0.31 +0.5 4,205,797 - LU632_RS22030 0.31 -0.1 4,205,859 + LU632_RS22030 0.36 +1.6 4,205,876 - LU632_RS22030 0.38 -0.2 4,205,891 + LU632_RS22030 0.39 +0.9 4,205,892 - LU632_RS22030 0.39 -0.2 4,206,050 + LU632_RS22030 0.54 +0.4 4,206,058 - LU632_RS22030 0.55 +0.3 4,206,087 + LU632_RS22030 0.57 +0.5 4,206,289 + LU632_RS22030 0.76 +0.2 4,206,290 - LU632_RS22030 0.76 +0.1 4,206,306 + LU632_RS22030 0.77 -0.4 4,206,307 - LU632_RS22030 0.77 +0.6
Or see this region's nucleotide sequence