Experiment: HBM-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfaF and rfaC overlap by 13 nucleotides rfaC and LU632_RS21930 are separated by 6 nucleotides LU632_RS21930 and LU632_RS21935 are separated by 42 nucleotides
LU632_RS21920: rfaF - ADP-heptose--LPS heptosyltransferase RfaF, at 4,186,918 to 4,187,979
rfaF
LU632_RS21925: rfaC - lipopolysaccharide heptosyltransferase RfaC, at 4,187,967 to 4,188,941
rfaC
LU632_RS21930: LU632_RS21930 - glycosyltransferase, at 4,188,948 to 4,189,865
_RS21930
LU632_RS21935: LU632_RS21935 - deacetylase, at 4,189,908 to 4,190,885
_RS21935
Position (kb)
4187
4188
4189 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4187.171 kb on - strand, within rfaF at 4187.776 kb on + strand, within rfaF at 4187.777 kb on - strand, within rfaF at 4188.007 kb on + strand at 4188.349 kb on - strand, within rfaC at 4188.395 kb on + strand, within rfaC at 4188.524 kb on + strand, within rfaC at 4188.524 kb on + strand, within rfaC at 4188.610 kb on + strand, within rfaC at 4188.610 kb on + strand, within rfaC at 4188.939 kb on + strand at 4188.999 kb on + strand at 4189.000 kb on - strand at 4189.011 kb on - strand at 4189.023 kb on - strand at 4189.023 kb on - strand at 4189.070 kb on + strand, within LU632_RS21930 at 4189.070 kb on + strand, within LU632_RS21930 at 4189.116 kb on + strand, within LU632_RS21930 at 4189.116 kb on + strand, within LU632_RS21930 at 4189.116 kb on + strand, within LU632_RS21930 at 4189.116 kb on + strand, within LU632_RS21930 at 4189.117 kb on - strand, within LU632_RS21930 at 4189.117 kb on - strand, within LU632_RS21930 at 4189.117 kb on - strand, within LU632_RS21930 at 4189.181 kb on + strand, within LU632_RS21930 at 4189.181 kb on + strand, within LU632_RS21930 at 4189.181 kb on + strand, within LU632_RS21930 at 4189.186 kb on - strand, within LU632_RS21930 at 4189.212 kb on + strand, within LU632_RS21930 at 4189.213 kb on - strand, within LU632_RS21930 at 4189.213 kb on - strand, within LU632_RS21930 at 4189.213 kb on - strand, within LU632_RS21930 at 4189.550 kb on + strand, within LU632_RS21930 at 4189.550 kb on + strand, within LU632_RS21930 at 4189.550 kb on + strand, within LU632_RS21930 at 4189.551 kb on - strand, within LU632_RS21930 at 4189.551 kb on - strand, within LU632_RS21930 at 4189.572 kb on + strand, within LU632_RS21930 at 4189.663 kb on + strand, within LU632_RS21930 at 4189.760 kb on - strand, within LU632_RS21930 at 4189.827 kb on - strand at 4189.827 kb on - strand at 4189.827 kb on - strand at 4189.846 kb on + strand at 4189.850 kb on + strand at 4189.850 kb on + strand at 4189.864 kb on - strand at 4189.919 kb on + strand at 4189.938 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Glucose remove 4,187,171 - rfaF LU632_RS21920 0.24 -0.5 4,187,776 + rfaF LU632_RS21920 0.81 +0.1 4,187,777 - rfaF LU632_RS21920 0.81 +1.1 4,188,007 + +0.1 4,188,349 - rfaC LU632_RS21925 0.39 +0.1 4,188,395 + rfaC LU632_RS21925 0.44 +1.1 4,188,524 + rfaC LU632_RS21925 0.57 +1.1 4,188,524 + rfaC LU632_RS21925 0.57 +0.1 4,188,610 + rfaC LU632_RS21925 0.66 -0.5 4,188,610 + rfaC LU632_RS21925 0.66 +0.1 4,188,939 + -0.3 4,188,999 + -0.7 4,189,000 - -2.6 4,189,011 - -0.8 4,189,023 - -0.1 4,189,023 - +0.5 4,189,070 + LU632_RS21930 0.13 -1.1 4,189,070 + LU632_RS21930 0.13 +0.1 4,189,116 + LU632_RS21930 0.18 -0.4 4,189,116 + LU632_RS21930 0.18 -0.5 4,189,116 + LU632_RS21930 0.18 -1.5 4,189,116 + LU632_RS21930 0.18 -0.4 4,189,117 - LU632_RS21930 0.18 -2.5 4,189,117 - LU632_RS21930 0.18 -1.1 4,189,117 - LU632_RS21930 0.18 +1.1 4,189,181 + LU632_RS21930 0.25 +0.5 4,189,181 + LU632_RS21930 0.25 -0.5 4,189,181 + LU632_RS21930 0.25 -1.2 4,189,186 - LU632_RS21930 0.26 -1.4 4,189,212 + LU632_RS21930 0.29 -0.7 4,189,213 - LU632_RS21930 0.29 +2.6 4,189,213 - LU632_RS21930 0.29 +0.8 4,189,213 - LU632_RS21930 0.29 +0.2 4,189,550 + LU632_RS21930 0.66 -0.9 4,189,550 + LU632_RS21930 0.66 +1.1 4,189,550 + LU632_RS21930 0.66 +0.3 4,189,551 - LU632_RS21930 0.66 -1.0 4,189,551 - LU632_RS21930 0.66 -0.9 4,189,572 + LU632_RS21930 0.68 +0.9 4,189,663 + LU632_RS21930 0.78 -0.6 4,189,760 - LU632_RS21930 0.88 -0.9 4,189,827 - -0.5 4,189,827 - -0.9 4,189,827 - -0.5 4,189,846 + -0.9 4,189,850 + +0.1 4,189,850 + -1.3 4,189,864 - -0.2 4,189,919 + +0.1 4,189,938 - -0.9
Or see this region's nucleotide sequence