Experiment: HBM-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gpsA and secB overlap by 1 nucleotides secB and grxC are separated by 42 nucleotides grxC and LU632_RS21890 are separated by 18 nucleotides LU632_RS21890 and envC are separated by 321 nucleotides
LU632_RS21875: gpsA - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase, at 4,178,865 to 4,179,884
gpsA
LU632_RS21880: secB - protein-export chaperone SecB, at 4,179,884 to 4,180,351
secB
LU632_RS21885: grxC - glutaredoxin 3, at 4,180,394 to 4,180,645
grxC
LU632_RS21890: LU632_RS21890 - rhodanese-like domain-containing protein, at 4,180,664 to 4,181,095
_RS21890
LU632_RS21895: envC - murein hydrolase activator EnvC, at 4,181,417 to 4,182,721
envC
Position (kb)
4180
4181
4182 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4179.714 kb on + strand, within gpsA at 4179.714 kb on + strand, within gpsA at 4179.714 kb on + strand, within gpsA at 4179.715 kb on - strand, within gpsA at 4179.735 kb on - strand, within gpsA at 4179.737 kb on + strand, within gpsA at 4179.738 kb on - strand, within gpsA at 4179.738 kb on - strand, within gpsA at 4179.776 kb on + strand, within gpsA at 4179.797 kb on + strand at 4179.798 kb on - strand at 4179.818 kb on + strand at 4179.818 kb on + strand at 4179.819 kb on - strand at 4179.885 kb on + strand at 4179.975 kb on - strand, within secB at 4179.975 kb on - strand, within secB at 4180.013 kb on - strand, within secB at 4180.050 kb on + strand, within secB at 4180.231 kb on + strand, within secB at 4180.231 kb on + strand, within secB at 4180.301 kb on - strand, within secB at 4180.301 kb on - strand, within secB at 4180.302 kb on + strand, within secB at 4180.303 kb on - strand, within secB at 4180.365 kb on - strand at 4180.396 kb on - strand at 4180.401 kb on - strand at 4180.475 kb on - strand, within grxC at 4180.546 kb on + strand, within grxC at 4180.754 kb on - strand, within LU632_RS21890 at 4180.953 kb on - strand, within LU632_RS21890 at 4181.025 kb on - strand, within LU632_RS21890 at 4181.083 kb on + strand at 4181.086 kb on + strand at 4181.086 kb on + strand at 4181.130 kb on + strand at 4181.192 kb on + strand at 4181.230 kb on - strand at 4181.376 kb on - strand at 4181.416 kb on + strand at 4181.518 kb on - strand at 4181.569 kb on + strand, within envC at 4181.570 kb on - strand, within envC at 4181.629 kb on - strand, within envC at 4181.903 kb on - strand, within envC at 4181.995 kb on - strand, within envC
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Glucose remove 4,179,714 + gpsA LU632_RS21875 0.83 -3.4 4,179,714 + gpsA LU632_RS21875 0.83 -0.9 4,179,714 + gpsA LU632_RS21875 0.83 -1.8 4,179,715 - gpsA LU632_RS21875 0.83 -1.8 4,179,735 - gpsA LU632_RS21875 0.85 -2.8 4,179,737 + gpsA LU632_RS21875 0.85 -4.1 4,179,738 - gpsA LU632_RS21875 0.86 -3.7 4,179,738 - gpsA LU632_RS21875 0.86 -3.0 4,179,776 + gpsA LU632_RS21875 0.89 -0.8 4,179,797 + -0.5 4,179,798 - -1.9 4,179,818 + -2.1 4,179,818 + -1.5 4,179,819 - -1.9 4,179,885 + -1.9 4,179,975 - secB LU632_RS21880 0.19 -0.9 4,179,975 - secB LU632_RS21880 0.19 +1.1 4,180,013 - secB LU632_RS21880 0.28 -4.5 4,180,050 + secB LU632_RS21880 0.35 -1.5 4,180,231 + secB LU632_RS21880 0.74 -1.5 4,180,231 + secB LU632_RS21880 0.74 -3.0 4,180,301 - secB LU632_RS21880 0.89 -1.9 4,180,301 - secB LU632_RS21880 0.89 -1.3 4,180,302 + secB LU632_RS21880 0.89 -1.3 4,180,303 - secB LU632_RS21880 0.90 -3.8 4,180,365 - -0.3 4,180,396 - +0.5 4,180,401 - +1.2 4,180,475 - grxC LU632_RS21885 0.32 +2.1 4,180,546 + grxC LU632_RS21885 0.60 -1.3 4,180,754 - LU632_RS21890 0.21 +0.5 4,180,953 - LU632_RS21890 0.67 +0.5 4,181,025 - LU632_RS21890 0.84 +1.1 4,181,083 + -4.1 4,181,086 + -0.4 4,181,086 + -2.0 4,181,130 + -3.3 4,181,192 + -1.6 4,181,230 - -0.1 4,181,376 - +1.2 4,181,416 + -0.1 4,181,518 - -0.1 4,181,569 + envC LU632_RS21895 0.12 +0.2 4,181,570 - envC LU632_RS21895 0.12 +0.4 4,181,629 - envC LU632_RS21895 0.16 -0.1 4,181,903 - envC LU632_RS21895 0.37 -0.1 4,181,995 - envC LU632_RS21895 0.44 -1.1
Or see this region's nucleotide sequence