Experiment: HBM-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt guaB and xseA are separated by 169 nucleotides xseA and LU632_RS17765 are separated by 80 nucleotides
LU632_RS17755: guaB - IMP dehydrogenase, at 3,339,760 to 3,341,226
guaB
LU632_RS17760: xseA - exodeoxyribonuclease VII large subunit, at 3,341,396 to 3,342,805
xseA
LU632_RS17765: LU632_RS17765 - M4 family metallopeptidase, at 3,342,886 to 3,343,507
_RS17765
Position (kb)
3341
3342
3343 Strain fitness (log2 ratio)
-1
0
1 at 3340.828 kb on - strand, within guaB at 3340.856 kb on - strand, within guaB at 3341.260 kb on + strand at 3341.282 kb on - strand at 3341.349 kb on + strand at 3341.353 kb on - strand at 3341.363 kb on + strand at 3341.440 kb on + strand at 3341.537 kb on + strand, within xseA at 3341.698 kb on + strand, within xseA at 3341.698 kb on + strand, within xseA at 3341.752 kb on + strand, within xseA at 3341.909 kb on + strand, within xseA at 3341.994 kb on + strand, within xseA at 3341.994 kb on + strand, within xseA at 3341.995 kb on - strand, within xseA at 3341.995 kb on - strand, within xseA at 3341.995 kb on - strand, within xseA at 3342.068 kb on - strand, within xseA at 3342.068 kb on - strand, within xseA at 3342.092 kb on - strand, within xseA at 3342.125 kb on - strand, within xseA at 3342.254 kb on + strand, within xseA at 3342.254 kb on + strand, within xseA at 3342.255 kb on - strand, within xseA at 3342.257 kb on + strand, within xseA at 3342.258 kb on - strand, within xseA at 3342.277 kb on + strand, within xseA at 3342.397 kb on - strand, within xseA at 3342.646 kb on + strand, within xseA at 3342.721 kb on + strand at 3342.722 kb on - strand at 3342.755 kb on + strand at 3342.756 kb on - strand at 3342.756 kb on - strand at 3342.758 kb on - strand at 3342.831 kb on + strand at 3342.831 kb on + strand at 3342.831 kb on + strand at 3342.831 kb on + strand at 3342.832 kb on - strand at 3342.832 kb on - strand at 3342.833 kb on + strand at 3342.834 kb on - strand at 3342.834 kb on - strand at 3342.834 kb on - strand at 3342.834 kb on - strand at 3342.870 kb on - strand at 3342.870 kb on - strand at 3342.921 kb on + strand at 3342.977 kb on - strand, within LU632_RS17765 at 3343.084 kb on + strand, within LU632_RS17765 at 3343.084 kb on + strand, within LU632_RS17765 at 3343.163 kb on + strand, within LU632_RS17765 at 3343.164 kb on - strand, within LU632_RS17765 at 3343.164 kb on - strand, within LU632_RS17765 at 3343.169 kb on + strand, within LU632_RS17765 at 3343.258 kb on + strand, within LU632_RS17765 at 3343.411 kb on + strand, within LU632_RS17765 at 3343.412 kb on - strand, within LU632_RS17765 at 3343.584 kb on + strand at 3343.585 kb on - strand at 3343.670 kb on - strand at 3343.689 kb on + strand at 3343.689 kb on + strand at 3343.689 kb on + strand at 3343.690 kb on - strand at 3343.790 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Glucose remove 3,340,828 - guaB LU632_RS17755 0.73 +0.8 3,340,856 - guaB LU632_RS17755 0.75 +0.8 3,341,260 + -1.2 3,341,282 - -1.5 3,341,349 + -0.5 3,341,353 - -1.0 3,341,363 + -0.2 3,341,440 + -0.6 3,341,537 + xseA LU632_RS17760 0.10 -0.1 3,341,698 + xseA LU632_RS17760 0.21 +0.8 3,341,698 + xseA LU632_RS17760 0.21 -0.7 3,341,752 + xseA LU632_RS17760 0.25 -0.5 3,341,909 + xseA LU632_RS17760 0.36 -0.6 3,341,994 + xseA LU632_RS17760 0.42 +0.1 3,341,994 + xseA LU632_RS17760 0.42 +0.6 3,341,995 - xseA LU632_RS17760 0.42 -0.2 3,341,995 - xseA LU632_RS17760 0.42 -0.4 3,341,995 - xseA LU632_RS17760 0.42 +1.4 3,342,068 - xseA LU632_RS17760 0.48 -0.9 3,342,068 - xseA LU632_RS17760 0.48 -0.3 3,342,092 - xseA LU632_RS17760 0.49 -0.7 3,342,125 - xseA LU632_RS17760 0.52 -0.3 3,342,254 + xseA LU632_RS17760 0.61 +0.3 3,342,254 + xseA LU632_RS17760 0.61 -0.6 3,342,255 - xseA LU632_RS17760 0.61 +0.3 3,342,257 + xseA LU632_RS17760 0.61 -0.2 3,342,258 - xseA LU632_RS17760 0.61 -1.2 3,342,277 + xseA LU632_RS17760 0.62 +0.0 3,342,397 - xseA LU632_RS17760 0.71 +1.4 3,342,646 + xseA LU632_RS17760 0.89 -1.0 3,342,721 + -0.4 3,342,722 - -0.3 3,342,755 + +0.8 3,342,756 - -0.5 3,342,756 - +0.4 3,342,758 - -0.4 3,342,831 + +1.1 3,342,831 + -0.3 3,342,831 + +0.3 3,342,831 + +0.2 3,342,832 - +0.7 3,342,832 - +0.5 3,342,833 + -0.4 3,342,834 - -0.2 3,342,834 - -0.4 3,342,834 - -0.1 3,342,834 - +0.2 3,342,870 - -0.1 3,342,870 - +0.1 3,342,921 + +0.5 3,342,977 - LU632_RS17765 0.15 -0.5 3,343,084 + LU632_RS17765 0.32 -0.2 3,343,084 + LU632_RS17765 0.32 +0.9 3,343,163 + LU632_RS17765 0.45 +0.6 3,343,164 - LU632_RS17765 0.45 -0.7 3,343,164 - LU632_RS17765 0.45 +0.7 3,343,169 + LU632_RS17765 0.45 -0.4 3,343,258 + LU632_RS17765 0.60 -0.2 3,343,411 + LU632_RS17765 0.84 -0.1 3,343,412 - LU632_RS17765 0.85 +0.3 3,343,584 + -0.5 3,343,585 - +0.8 3,343,670 - +0.3 3,343,689 + +0.1 3,343,689 + -0.2 3,343,689 + +0.5 3,343,690 - +0.8 3,343,790 + -0.1
Or see this region's nucleotide sequence