Experiment: HBM-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt guaD and tnpA are separated by 156 nucleotides tnpA and LU632_RS12490 are separated by 48 nucleotides LU632_RS12490 and yciH are separated by 55 nucleotides yciH and pyrF overlap by 1 nucleotides
LU632_RS12480: guaD - guanine deaminase, at 2,370,733 to 2,371,958
guaD
LU632_RS12485: tnpA - IS200/IS605 family transposase, at 2,372,115 to 2,372,531
tnpA
LU632_RS12490: LU632_RS12490 - IS256 family transposase, at 2,372,580 to 2,373,787
_RS12490
LU632_RS12495: yciH - stress response translation initiation inhibitor YciH, at 2,373,843 to 2,374,169
yciH
LU632_RS12500: pyrF - orotidine-5'-phosphate decarboxylase, at 2,374,169 to 2,374,885
pyrF
Position (kb)
2372
2373
2374 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 2371.670 kb on + strand, within guaD at 2371.770 kb on + strand, within guaD at 2371.857 kb on - strand at 2371.883 kb on - strand at 2371.883 kb on - strand at 2372.461 kb on + strand, within tnpA at 2372.465 kb on + strand, within tnpA at 2372.465 kb on + strand, within tnpA at 2372.467 kb on + strand, within tnpA at 2372.520 kb on - strand at 2372.520 kb on - strand at 2373.799 kb on + strand at 2373.834 kb on - strand at 2373.867 kb on - strand at 2374.005 kb on + strand, within yciH at 2374.248 kb on - strand, within pyrF at 2374.253 kb on - strand, within pyrF at 2374.253 kb on - strand, within pyrF at 2374.254 kb on + strand, within pyrF at 2374.254 kb on + strand, within pyrF at 2374.333 kb on + strand, within pyrF at 2374.469 kb on - strand, within pyrF at 2374.523 kb on + strand, within pyrF at 2374.658 kb on - strand, within pyrF at 2374.658 kb on - strand, within pyrF at 2374.663 kb on + strand, within pyrF at 2374.664 kb on - strand, within pyrF at 2374.666 kb on + strand, within pyrF at 2374.667 kb on - strand, within pyrF at 2374.676 kb on - strand, within pyrF at 2374.676 kb on - strand, within pyrF
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Glucose remove 2,371,670 + guaD LU632_RS12480 0.76 -1.0 2,371,770 + guaD LU632_RS12480 0.85 +0.1 2,371,857 - -0.1 2,371,883 - +2.6 2,371,883 - -0.8 2,372,461 + tnpA LU632_RS12485 0.83 +0.1 2,372,465 + tnpA LU632_RS12485 0.84 +1.1 2,372,465 + tnpA LU632_RS12485 0.84 +0.2 2,372,467 + tnpA LU632_RS12485 0.84 +0.0 2,372,520 - +0.5 2,372,520 - -0.9 2,373,799 + +1.6 2,373,834 - -0.5 2,373,867 - +0.0 2,374,005 + yciH LU632_RS12495 0.50 +0.2 2,374,248 - pyrF LU632_RS12500 0.11 -6.5 2,374,253 - pyrF LU632_RS12500 0.12 -1.4 2,374,253 - pyrF LU632_RS12500 0.12 -3.5 2,374,254 + pyrF LU632_RS12500 0.12 -3.2 2,374,254 + pyrF LU632_RS12500 0.12 -3.6 2,374,333 + pyrF LU632_RS12500 0.23 -4.3 2,374,469 - pyrF LU632_RS12500 0.42 -2.2 2,374,523 + pyrF LU632_RS12500 0.49 -1.4 2,374,658 - pyrF LU632_RS12500 0.68 -2.2 2,374,658 - pyrF LU632_RS12500 0.68 -3.6 2,374,663 + pyrF LU632_RS12500 0.69 -3.9 2,374,664 - pyrF LU632_RS12500 0.69 -2.2 2,374,666 + pyrF LU632_RS12500 0.69 -1.4 2,374,667 - pyrF LU632_RS12500 0.69 -2.5 2,374,676 - pyrF LU632_RS12500 0.71 -3.6 2,374,676 - pyrF LU632_RS12500 0.71 -3.2
Or see this region's nucleotide sequence