Experiment: HBM-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uvrC and pgsA are separated by 71 nucleotides pgsA and LU632_RS10205 are separated by 148 nucleotides LU632_RS10205 and LU632_RS10210 are separated by 42 nucleotides LU632_RS10210 and LU632_RS10215 are separated by 12 nucleotides LU632_RS10215 and LU632_RS10220 are separated by 163 nucleotides
LU632_RS10195: uvrC - excinuclease ABC subunit UvrC, at 1,950,793 to 1,952,625
uvrC
LU632_RS10200: pgsA - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 1,952,697 to 1,953,245
pgsA
LU632_RS10205: LU632_RS10205 - tRNA-Gly, at 1,953,394 to 1,953,469
_RS10205
LU632_RS10210: LU632_RS10210 - tRNA-Cys, at 1,953,512 to 1,953,585
_RS10210
LU632_RS10215: LU632_RS10215 - tRNA-Leu, at 1,953,598 to 1,953,683
_RS10215
LU632_RS10220: LU632_RS10220 - integrase arm-type DNA-binding domain-containing protein, at 1,953,847 to 1,954,824
_RS10220
Position (kb)
1952
1953
1954 Strain fitness (log2 ratio)
-1
0
1 at 1951.852 kb on + strand, within uvrC at 1951.990 kb on - strand, within uvrC at 1951.992 kb on + strand, within uvrC at 1951.998 kb on + strand, within uvrC at 1951.999 kb on - strand, within uvrC at 1951.999 kb on - strand, within uvrC at 1951.999 kb on - strand, within uvrC at 1951.999 kb on - strand, within uvrC at 1952.141 kb on - strand, within uvrC at 1952.163 kb on + strand, within uvrC at 1952.164 kb on - strand, within uvrC at 1952.207 kb on + strand, within uvrC at 1952.208 kb on - strand, within uvrC at 1952.388 kb on + strand, within uvrC at 1952.511 kb on + strand at 1952.512 kb on - strand at 1952.516 kb on - strand at 1952.516 kb on - strand at 1952.608 kb on + strand at 1952.616 kb on - strand at 1952.649 kb on + strand at 1952.649 kb on + strand at 1952.657 kb on + strand at 1952.657 kb on + strand at 1953.211 kb on + strand at 1953.211 kb on + strand at 1953.211 kb on + strand at 1953.686 kb on - strand at 1953.686 kb on - strand at 1953.901 kb on - strand at 1953.901 kb on - strand at 1953.925 kb on + strand at 1953.955 kb on + strand, within LU632_RS10220 at 1954.017 kb on - strand, within LU632_RS10220 at 1954.050 kb on + strand, within LU632_RS10220 at 1954.138 kb on + strand, within LU632_RS10220 at 1954.139 kb on - strand, within LU632_RS10220 at 1954.139 kb on - strand, within LU632_RS10220 at 1954.177 kb on - strand, within LU632_RS10220 at 1954.177 kb on - strand, within LU632_RS10220 at 1954.181 kb on + strand, within LU632_RS10220 at 1954.182 kb on - strand, within LU632_RS10220 at 1954.233 kb on + strand, within LU632_RS10220 at 1954.234 kb on - strand, within LU632_RS10220
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Glucose remove 1,951,852 + uvrC LU632_RS10195 0.58 +0.9 1,951,990 - uvrC LU632_RS10195 0.65 +1.0 1,951,992 + uvrC LU632_RS10195 0.65 -0.9 1,951,998 + uvrC LU632_RS10195 0.66 -0.0 1,951,999 - uvrC LU632_RS10195 0.66 +0.1 1,951,999 - uvrC LU632_RS10195 0.66 -0.4 1,951,999 - uvrC LU632_RS10195 0.66 +0.2 1,951,999 - uvrC LU632_RS10195 0.66 -1.3 1,952,141 - uvrC LU632_RS10195 0.74 -0.9 1,952,163 + uvrC LU632_RS10195 0.75 -0.5 1,952,164 - uvrC LU632_RS10195 0.75 -0.3 1,952,207 + uvrC LU632_RS10195 0.77 -0.9 1,952,208 - uvrC LU632_RS10195 0.77 -0.2 1,952,388 + uvrC LU632_RS10195 0.87 -0.6 1,952,511 + +0.1 1,952,512 - -1.2 1,952,516 - -0.4 1,952,516 - +1.0 1,952,608 + +0.7 1,952,616 - +0.0 1,952,649 + -1.6 1,952,649 + -0.3 1,952,657 + -1.4 1,952,657 + +1.3 1,953,211 + -0.2 1,953,211 + +0.2 1,953,211 + +0.6 1,953,686 - +0.5 1,953,686 - +0.5 1,953,901 - +0.7 1,953,901 - +0.3 1,953,925 + +0.6 1,953,955 + LU632_RS10220 0.11 +0.3 1,954,017 - LU632_RS10220 0.17 -0.5 1,954,050 + LU632_RS10220 0.21 -0.4 1,954,138 + LU632_RS10220 0.30 -0.7 1,954,139 - LU632_RS10220 0.30 -0.1 1,954,139 - LU632_RS10220 0.30 -0.2 1,954,177 - LU632_RS10220 0.34 -0.3 1,954,177 - LU632_RS10220 0.34 +1.7 1,954,181 + LU632_RS10220 0.34 -1.0 1,954,182 - LU632_RS10220 0.34 -0.0 1,954,233 + LU632_RS10220 0.39 -0.2 1,954,234 - LU632_RS10220 0.40 -0.5
Or see this region's nucleotide sequence