Experiment: HBM-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rutA and rutR are separated by 282 nucleotides rutR and cycA are separated by 63 nucleotides
LU632_RS10150: rutA - pyrimidine utilization protein A, at 1,941,039 to 1,942,133
rutA
LU632_RS10155: rutR - HTH-type transcriptional regulator RutR, at 1,942,416 to 1,942,999
rutR
LU632_RS10160: cycA - D-serine/D-alanine/glycine transporter, at 1,943,063 to 1,944,460
cycA
Position (kb)
1943
1944
1945 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1942.064 kb on + strand at 1942.065 kb on - strand at 1942.065 kb on - strand at 1942.066 kb on + strand at 1942.066 kb on + strand at 1942.067 kb on - strand at 1942.067 kb on - strand at 1942.067 kb on - strand at 1942.067 kb on - strand at 1942.116 kb on - strand at 1942.119 kb on + strand at 1942.144 kb on + strand at 1942.160 kb on + strand at 1942.163 kb on + strand at 1942.180 kb on - strand at 1942.183 kb on + strand at 1942.184 kb on - strand at 1942.184 kb on - strand at 1942.362 kb on - strand at 1942.406 kb on + strand at 1942.551 kb on + strand, within rutR at 1942.594 kb on - strand, within rutR at 1942.620 kb on + strand, within rutR at 1942.621 kb on - strand, within rutR at 1942.622 kb on + strand, within rutR at 1942.667 kb on + strand, within rutR at 1942.667 kb on + strand, within rutR at 1942.667 kb on + strand, within rutR at 1942.667 kb on + strand, within rutR at 1942.667 kb on + strand, within rutR at 1942.668 kb on - strand, within rutR at 1942.668 kb on - strand, within rutR at 1942.752 kb on + strand, within rutR at 1942.988 kb on - strand at 1943.010 kb on + strand at 1943.011 kb on - strand at 1943.121 kb on - strand at 1943.177 kb on + strand at 1943.177 kb on + strand at 1943.212 kb on + strand, within cycA at 1943.212 kb on + strand, within cycA at 1943.212 kb on + strand, within cycA at 1943.213 kb on - strand, within cycA at 1943.213 kb on - strand, within cycA at 1943.213 kb on - strand, within cycA at 1943.213 kb on - strand, within cycA at 1943.213 kb on - strand, within cycA at 1943.213 kb on - strand, within cycA at 1943.223 kb on - strand, within cycA at 1943.236 kb on - strand, within cycA at 1943.252 kb on + strand, within cycA at 1943.253 kb on - strand, within cycA at 1943.283 kb on + strand, within cycA at 1943.283 kb on + strand, within cycA at 1943.314 kb on + strand, within cycA at 1943.377 kb on - strand, within cycA at 1943.467 kb on + strand, within cycA at 1943.496 kb on + strand, within cycA at 1943.496 kb on + strand, within cycA at 1943.496 kb on + strand, within cycA at 1943.497 kb on - strand, within cycA at 1943.497 kb on - strand, within cycA at 1943.686 kb on - strand, within cycA at 1943.695 kb on + strand, within cycA at 1943.741 kb on - strand, within cycA at 1943.848 kb on + strand, within cycA at 1943.849 kb on - strand, within cycA at 1943.885 kb on - strand, within cycA at 1943.887 kb on + strand, within cycA at 1943.888 kb on - strand, within cycA at 1943.910 kb on - strand, within cycA at 1943.996 kb on + strand, within cycA at 1944.001 kb on + strand, within cycA at 1944.002 kb on - strand, within cycA at 1944.233 kb on + strand, within cycA at 1944.278 kb on - strand, within cycA at 1944.278 kb on - strand, within cycA at 1944.283 kb on + strand, within cycA at 1944.285 kb on + strand, within cycA at 1944.375 kb on - strand at 1944.375 kb on - strand at 1944.382 kb on + strand at 1944.382 kb on + strand at 1944.446 kb on + strand at 1944.483 kb on - strand at 1944.510 kb on - strand at 1944.548 kb on - strand at 1944.552 kb on + strand at 1944.586 kb on - strand at 1944.682 kb on - strand at 1944.870 kb on + strand at 1944.982 kb on + strand at 1945.100 kb on + strand at 1945.100 kb on + strand at 1945.100 kb on + strand at 1945.100 kb on + strand at 1945.101 kb on - strand at 1945.101 kb on - strand at 1945.187 kb on + strand at 1945.286 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Glucose remove 1,942,064 + +0.3 1,942,065 - -0.1 1,942,065 - +1.2 1,942,066 + -0.5 1,942,066 + +0.7 1,942,067 - -0.2 1,942,067 - -0.3 1,942,067 - +1.0 1,942,067 - +1.0 1,942,116 - -0.3 1,942,119 + -0.2 1,942,144 + -0.5 1,942,160 + +1.7 1,942,163 + -0.5 1,942,180 - -0.4 1,942,183 + +0.6 1,942,184 - -0.3 1,942,184 - -1.3 1,942,362 - -0.9 1,942,406 + -0.2 1,942,551 + rutR LU632_RS10155 0.23 -0.0 1,942,594 - rutR LU632_RS10155 0.30 -0.0 1,942,620 + rutR LU632_RS10155 0.35 +0.4 1,942,621 - rutR LU632_RS10155 0.35 -0.6 1,942,622 + rutR LU632_RS10155 0.35 -0.5 1,942,667 + rutR LU632_RS10155 0.43 +0.1 1,942,667 + rutR LU632_RS10155 0.43 -0.9 1,942,667 + rutR LU632_RS10155 0.43 -0.5 1,942,667 + rutR LU632_RS10155 0.43 +0.4 1,942,667 + rutR LU632_RS10155 0.43 +1.4 1,942,668 - rutR LU632_RS10155 0.43 -1.5 1,942,668 - rutR LU632_RS10155 0.43 +0.1 1,942,752 + rutR LU632_RS10155 0.58 -0.0 1,942,988 - -0.6 1,943,010 + +0.4 1,943,011 - +0.3 1,943,121 - -0.3 1,943,177 + +0.4 1,943,177 + +1.5 1,943,212 + cycA LU632_RS10160 0.11 +0.4 1,943,212 + cycA LU632_RS10160 0.11 -0.1 1,943,212 + cycA LU632_RS10160 0.11 +0.7 1,943,213 - cycA LU632_RS10160 0.11 +0.6 1,943,213 - cycA LU632_RS10160 0.11 -0.1 1,943,213 - cycA LU632_RS10160 0.11 -0.1 1,943,213 - cycA LU632_RS10160 0.11 -0.4 1,943,213 - cycA LU632_RS10160 0.11 -0.4 1,943,213 - cycA LU632_RS10160 0.11 +0.2 1,943,223 - cycA LU632_RS10160 0.11 +0.4 1,943,236 - cycA LU632_RS10160 0.12 -1.8 1,943,252 + cycA LU632_RS10160 0.14 +0.0 1,943,253 - cycA LU632_RS10160 0.14 -0.4 1,943,283 + cycA LU632_RS10160 0.16 -1.5 1,943,283 + cycA LU632_RS10160 0.16 -1.0 1,943,314 + cycA LU632_RS10160 0.18 -3.0 1,943,377 - cycA LU632_RS10160 0.22 +1.3 1,943,467 + cycA LU632_RS10160 0.29 -1.1 1,943,496 + cycA LU632_RS10160 0.31 -0.1 1,943,496 + cycA LU632_RS10160 0.31 -0.1 1,943,496 + cycA LU632_RS10160 0.31 +0.5 1,943,497 - cycA LU632_RS10160 0.31 -0.3 1,943,497 - cycA LU632_RS10160 0.31 -0.6 1,943,686 - cycA LU632_RS10160 0.45 -0.6 1,943,695 + cycA LU632_RS10160 0.45 +0.1 1,943,741 - cycA LU632_RS10160 0.48 -1.2 1,943,848 + cycA LU632_RS10160 0.56 -0.5 1,943,849 - cycA LU632_RS10160 0.56 -0.2 1,943,885 - cycA LU632_RS10160 0.59 -1.1 1,943,887 + cycA LU632_RS10160 0.59 -1.5 1,943,888 - cycA LU632_RS10160 0.59 +0.5 1,943,910 - cycA LU632_RS10160 0.61 -0.0 1,943,996 + cycA LU632_RS10160 0.67 -0.2 1,944,001 + cycA LU632_RS10160 0.67 +0.2 1,944,002 - cycA LU632_RS10160 0.67 +0.0 1,944,233 + cycA LU632_RS10160 0.84 -0.5 1,944,278 - cycA LU632_RS10160 0.87 +0.2 1,944,278 - cycA LU632_RS10160 0.87 -0.9 1,944,283 + cycA LU632_RS10160 0.87 -0.5 1,944,285 + cycA LU632_RS10160 0.87 -0.0 1,944,375 - -1.2 1,944,375 - +0.0 1,944,382 + -0.3 1,944,382 + -0.5 1,944,446 + -0.1 1,944,483 - +0.1 1,944,510 - -0.1 1,944,548 - +0.2 1,944,552 + -1.2 1,944,586 - -0.1 1,944,682 - -0.7 1,944,870 + -0.2 1,944,982 + -0.8 1,945,100 + -1.2 1,945,100 + +0.0 1,945,100 + +0.1 1,945,100 + +1.1 1,945,101 - -0.7 1,945,101 - -0.3 1,945,187 + -0.1 1,945,286 - -0.3
Or see this region's nucleotide sequence