Experiment: HBM-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pbpG and LU632_RS09270 are separated by 93 nucleotides LU632_RS09270 and LU632_RS09275 are separated by 39 nucleotides LU632_RS09275 and LU632_RS09280 overlap by 10 nucleotides LU632_RS09280 and LU632_RS09285 are separated by 21 nucleotides
LU632_RS09265: pbpG - D-alanyl-D-alanine endopeptidase, at 1,748,492 to 1,749,433
pbpG
LU632_RS09270: LU632_RS09270 - DUF3772 domain-containing protein, at 1,749,527 to 1,751,965
_RS09270
LU632_RS09275: LU632_RS09275 - hypothetical protein, at 1,752,005 to 1,752,235
_RS09275
LU632_RS09280: LU632_RS09280 - tautomerase family protein, at 1,752,226 to 1,752,453
_RS09280
LU632_RS09285: LU632_RS09285 - pseudouridine-5'-phosphate glycosidase, at 1,752,475 to 1,753,410
_RS09285
Position (kb)
1749
1750
1751
1752 Strain fitness (log2 ratio)
-1
0
1
2 at 1748.661 kb on - strand, within pbpG at 1748.661 kb on - strand, within pbpG at 1748.661 kb on - strand, within pbpG at 1748.661 kb on - strand, within pbpG at 1748.802 kb on - strand, within pbpG at 1748.842 kb on + strand, within pbpG at 1749.039 kb on + strand, within pbpG at 1749.059 kb on + strand, within pbpG at 1749.122 kb on + strand, within pbpG at 1749.123 kb on - strand, within pbpG at 1749.123 kb on - strand, within pbpG at 1749.175 kb on + strand, within pbpG at 1749.176 kb on - strand, within pbpG at 1749.288 kb on - strand, within pbpG at 1749.288 kb on - strand, within pbpG at 1749.371 kb on + strand at 1749.373 kb on + strand at 1749.374 kb on - strand at 1749.436 kb on - strand at 1749.436 kb on - strand at 1749.464 kb on - strand at 1749.820 kb on - strand, within LU632_RS09270 at 1749.870 kb on + strand, within LU632_RS09270 at 1750.378 kb on + strand, within LU632_RS09270 at 1750.378 kb on + strand, within LU632_RS09270 at 1750.378 kb on + strand, within LU632_RS09270 at 1750.432 kb on + strand, within LU632_RS09270 at 1750.709 kb on - strand, within LU632_RS09270 at 1750.848 kb on - strand, within LU632_RS09270 at 1750.886 kb on + strand, within LU632_RS09270 at 1750.889 kb on - strand, within LU632_RS09270 at 1750.889 kb on - strand, within LU632_RS09270 at 1750.939 kb on + strand, within LU632_RS09270 at 1750.939 kb on + strand, within LU632_RS09270 at 1750.940 kb on - strand, within LU632_RS09270 at 1750.940 kb on - strand, within LU632_RS09270 at 1750.940 kb on - strand, within LU632_RS09270 at 1751.170 kb on - strand, within LU632_RS09270 at 1751.201 kb on - strand, within LU632_RS09270 at 1751.204 kb on + strand, within LU632_RS09270 at 1751.205 kb on - strand, within LU632_RS09270 at 1751.205 kb on - strand, within LU632_RS09270 at 1751.208 kb on + strand, within LU632_RS09270 at 1751.209 kb on - strand, within LU632_RS09270 at 1751.312 kb on + strand, within LU632_RS09270 at 1751.470 kb on - strand, within LU632_RS09270 at 1751.492 kb on - strand, within LU632_RS09270 at 1751.591 kb on - strand, within LU632_RS09270 at 1751.887 kb on - strand at 1751.989 kb on + strand at 1751.990 kb on - strand at 1752.034 kb on - strand, within LU632_RS09275 at 1752.123 kb on + strand, within LU632_RS09275 at 1752.158 kb on - strand, within LU632_RS09275 at 1752.227 kb on + strand at 1752.227 kb on + strand at 1752.227 kb on + strand at 1752.227 kb on + strand at 1752.228 kb on - strand at 1752.228 kb on - strand at 1752.228 kb on - strand at 1752.228 kb on - strand at 1752.228 kb on - strand at 1752.228 kb on - strand at 1752.228 kb on - strand at 1752.233 kb on + strand at 1752.234 kb on - strand at 1752.274 kb on - strand, within LU632_RS09280 at 1752.274 kb on - strand, within LU632_RS09280 at 1752.275 kb on + strand, within LU632_RS09280 at 1752.275 kb on + strand, within LU632_RS09280 at 1752.275 kb on + strand, within LU632_RS09280 at 1752.500 kb on + strand at 1752.542 kb on - strand at 1752.893 kb on + strand, within LU632_RS09285 at 1752.894 kb on - strand, within LU632_RS09285 at 1752.943 kb on + strand, within LU632_RS09285
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Glucose remove 1,748,661 - pbpG LU632_RS09265 0.18 +0.2 1,748,661 - pbpG LU632_RS09265 0.18 +0.1 1,748,661 - pbpG LU632_RS09265 0.18 +0.6 1,748,661 - pbpG LU632_RS09265 0.18 -0.8 1,748,802 - pbpG LU632_RS09265 0.33 +0.3 1,748,842 + pbpG LU632_RS09265 0.37 -0.0 1,749,039 + pbpG LU632_RS09265 0.58 -0.4 1,749,059 + pbpG LU632_RS09265 0.60 -0.0 1,749,122 + pbpG LU632_RS09265 0.67 -0.0 1,749,123 - pbpG LU632_RS09265 0.67 -0.6 1,749,123 - pbpG LU632_RS09265 0.67 +0.3 1,749,175 + pbpG LU632_RS09265 0.73 +0.2 1,749,176 - pbpG LU632_RS09265 0.73 -0.2 1,749,288 - pbpG LU632_RS09265 0.85 +0.5 1,749,288 - pbpG LU632_RS09265 0.85 -0.0 1,749,371 + +0.2 1,749,373 + -1.0 1,749,374 - +1.3 1,749,436 - -0.2 1,749,436 - -0.2 1,749,464 - +2.6 1,749,820 - LU632_RS09270 0.12 -0.2 1,749,870 + LU632_RS09270 0.14 +0.1 1,750,378 + LU632_RS09270 0.35 +0.7 1,750,378 + LU632_RS09270 0.35 -0.1 1,750,378 + LU632_RS09270 0.35 +0.2 1,750,432 + LU632_RS09270 0.37 -0.2 1,750,709 - LU632_RS09270 0.48 -1.2 1,750,848 - LU632_RS09270 0.54 -0.2 1,750,886 + LU632_RS09270 0.56 +0.3 1,750,889 - LU632_RS09270 0.56 +0.1 1,750,889 - LU632_RS09270 0.56 +0.6 1,750,939 + LU632_RS09270 0.58 +0.3 1,750,939 + LU632_RS09270 0.58 +0.1 1,750,940 - LU632_RS09270 0.58 -0.0 1,750,940 - LU632_RS09270 0.58 -0.1 1,750,940 - LU632_RS09270 0.58 +1.3 1,751,170 - LU632_RS09270 0.67 -0.5 1,751,201 - LU632_RS09270 0.69 -0.0 1,751,204 + LU632_RS09270 0.69 -0.2 1,751,205 - LU632_RS09270 0.69 +1.3 1,751,205 - LU632_RS09270 0.69 +0.2 1,751,208 + LU632_RS09270 0.69 -0.1 1,751,209 - LU632_RS09270 0.69 +0.0 1,751,312 + LU632_RS09270 0.73 -0.1 1,751,470 - LU632_RS09270 0.80 +0.8 1,751,492 - LU632_RS09270 0.81 +0.7 1,751,591 - LU632_RS09270 0.85 -0.6 1,751,887 - +0.0 1,751,989 + -0.4 1,751,990 - +1.0 1,752,034 - LU632_RS09275 0.13 -0.7 1,752,123 + LU632_RS09275 0.51 -1.2 1,752,158 - LU632_RS09275 0.66 +1.2 1,752,227 + -0.4 1,752,227 + +0.2 1,752,227 + +0.5 1,752,227 + +0.3 1,752,228 - -0.2 1,752,228 - -0.1 1,752,228 - +0.1 1,752,228 - +0.6 1,752,228 - -0.5 1,752,228 - +0.0 1,752,228 - +0.2 1,752,233 + +0.8 1,752,234 - -0.5 1,752,274 - LU632_RS09280 0.21 -0.2 1,752,274 - LU632_RS09280 0.21 -0.4 1,752,275 + LU632_RS09280 0.21 +0.9 1,752,275 + LU632_RS09280 0.21 -0.4 1,752,275 + LU632_RS09280 0.21 +0.2 1,752,500 + -0.4 1,752,542 - -0.6 1,752,893 + LU632_RS09285 0.45 +0.3 1,752,894 - LU632_RS09285 0.45 +1.0 1,752,943 + LU632_RS09285 0.50 +0.1
Or see this region's nucleotide sequence