Experiment: HBM-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gcvP and LU632_RS05920 are separated by 263 nucleotides LU632_RS05920 and LU632_RS05925 are separated by 133 nucleotides LU632_RS05925 and LU632_RS05930 are separated by 273 nucleotides
LU632_RS05915: gcvP - aminomethyl-transferring glycine dehydrogenase, at 1,121,632 to 1,124,514
gcvP
LU632_RS05920: LU632_RS05920 - hypothetical protein, at 1,124,778 to 1,125,068
_RS05920
LU632_RS05925: LU632_RS05925 - transcriptional regulator, at 1,125,202 to 1,125,312
_RS05925
LU632_RS05930: LU632_RS05930 - 6-phospho-beta-glucosidase, at 1,125,586 to 1,127,025
_RS05930
Position (kb) 1124
1125
1126 Strain fitness (log2 ratio) -1
0
1 at 1123.936 kb on + strand, within gcvP at 1123.936 kb on + strand, within gcvP at 1124.003 kb on - strand, within gcvP at 1124.039 kb on + strand, within gcvP at 1124.039 kb on + strand, within gcvP at 1124.041 kb on + strand, within gcvP at 1124.075 kb on + strand, within gcvP at 1124.077 kb on - strand, within gcvP at 1124.077 kb on - strand, within gcvP at 1124.244 kb on + strand at 1124.245 kb on - strand at 1124.245 kb on - strand at 1124.306 kb on + strand at 1124.374 kb on + strand at 1124.375 kb on - strand at 1124.375 kb on - strand at 1124.415 kb on + strand at 1124.416 kb on - strand at 1124.416 kb on - strand at 1124.416 kb on - strand at 1124.419 kb on + strand at 1124.529 kb on - strand at 1124.556 kb on - strand at 1124.564 kb on + strand at 1124.568 kb on + strand at 1124.635 kb on - strand at 1124.717 kb on + strand at 1124.717 kb on + strand at 1124.717 kb on + strand at 1124.717 kb on + strand at 1124.718 kb on - strand at 1124.813 kb on + strand, within LU632_RS05920 at 1124.813 kb on + strand, within LU632_RS05920 at 1124.852 kb on - strand, within LU632_RS05920 at 1124.852 kb on - strand, within LU632_RS05920 at 1124.967 kb on - strand, within LU632_RS05920 at 1125.030 kb on - strand, within LU632_RS05920 at 1125.030 kb on - strand, within LU632_RS05920 at 1125.042 kb on - strand at 1125.122 kb on + strand at 1125.122 kb on + strand at 1125.123 kb on - strand at 1125.123 kb on - strand at 1125.182 kb on - strand at 1125.204 kb on - strand at 1125.279 kb on - strand, within LU632_RS05925 at 1125.295 kb on - strand, within LU632_RS05925 at 1125.328 kb on - strand at 1125.335 kb on + strand at 1125.357 kb on - strand at 1125.357 kb on - strand at 1125.370 kb on + strand at 1125.370 kb on + strand at 1125.370 kb on + strand at 1125.370 kb on + strand at 1125.371 kb on - strand at 1125.371 kb on - strand at 1125.371 kb on - strand at 1125.371 kb on - strand at 1125.395 kb on - strand at 1125.490 kb on - strand at 1125.534 kb on + strand at 1125.534 kb on + strand at 1125.535 kb on - strand at 1125.621 kb on + strand at 1125.623 kb on + strand at 1125.786 kb on - strand, within LU632_RS05930 at 1125.947 kb on + strand, within LU632_RS05930 at 1125.948 kb on - strand, within LU632_RS05930
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Glucose remove 1,123,936 + gcvP LU632_RS05915 0.80 -1.2 1,123,936 + gcvP LU632_RS05915 0.80 -0.9 1,124,003 - gcvP LU632_RS05915 0.82 -0.5 1,124,039 + gcvP LU632_RS05915 0.83 -1.0 1,124,039 + gcvP LU632_RS05915 0.83 +1.5 1,124,041 + gcvP LU632_RS05915 0.84 -0.6 1,124,075 + gcvP LU632_RS05915 0.85 -0.3 1,124,077 - gcvP LU632_RS05915 0.85 -1.7 1,124,077 - gcvP LU632_RS05915 0.85 -0.6 1,124,244 + +0.5 1,124,245 - +0.3 1,124,245 - +0.9 1,124,306 + -1.2 1,124,374 + -0.8 1,124,375 - -0.4 1,124,375 - -1.4 1,124,415 + -1.0 1,124,416 - -1.0 1,124,416 - -1.4 1,124,416 - -1.7 1,124,419 + -0.7 1,124,529 - +0.2 1,124,556 - -0.4 1,124,564 + -0.7 1,124,568 + -0.3 1,124,635 - +0.1 1,124,717 + -0.3 1,124,717 + +0.4 1,124,717 + -0.3 1,124,717 + -1.1 1,124,718 - -1.4 1,124,813 + LU632_RS05920 0.12 +0.6 1,124,813 + LU632_RS05920 0.12 +0.0 1,124,852 - LU632_RS05920 0.25 +0.4 1,124,852 - LU632_RS05920 0.25 -0.6 1,124,967 - LU632_RS05920 0.65 +0.7 1,125,030 - LU632_RS05920 0.87 +0.6 1,125,030 - LU632_RS05920 0.87 -0.1 1,125,042 - +0.6 1,125,122 + +1.6 1,125,122 + +0.7 1,125,123 - +0.2 1,125,123 - +0.9 1,125,182 - +0.3 1,125,204 - +0.0 1,125,279 - LU632_RS05925 0.69 -1.0 1,125,295 - LU632_RS05925 0.84 +0.5 1,125,328 - +0.4 1,125,335 + +0.3 1,125,357 - -0.6 1,125,357 - +0.1 1,125,370 + +0.7 1,125,370 + +0.9 1,125,370 + +0.1 1,125,370 + +0.9 1,125,371 - -0.2 1,125,371 - +0.6 1,125,371 - +0.2 1,125,371 - +0.1 1,125,395 - +0.9 1,125,490 - +0.3 1,125,534 + -0.1 1,125,534 + -0.7 1,125,535 - -0.6 1,125,621 + -0.6 1,125,623 + -0.1 1,125,786 - LU632_RS05930 0.14 -0.3 1,125,947 + LU632_RS05930 0.25 -0.1 1,125,948 - LU632_RS05930 0.25 -0.1
Or see this region's nucleotide sequence