Experiment: HBM-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sctC and LU632_RS03245 are separated by 14 nucleotides LU632_RS03245 and LU632_RS03250 overlap by 8 nucleotides LU632_RS03250 and LU632_RS03255 are separated by 189 nucleotides
LU632_RS03240: sctC - type III secretion system outer membrane ring subunit SctC, at 660,144 to 662,168
sctC
LU632_RS03245: LU632_RS03245 - hypothetical protein, at 662,183 to 662,377
_RS03245
LU632_RS03250: LU632_RS03250 - type III secretion system protein, at 662,370 to 662,720
_RS03250
LU632_RS03255: LU632_RS03255 - harpin HrpZ family protein, at 662,910 to 664,127
_RS03255
Position (kb) 662
663 Strain fitness (log2 ratio) -2
-1
0
1
2 at 661.200 kb on - strand, within sctC at 661.250 kb on + strand, within sctC at 661.251 kb on - strand, within sctC at 661.251 kb on - strand, within sctC at 661.320 kb on + strand, within sctC at 661.321 kb on - strand, within sctC at 661.326 kb on - strand, within sctC at 661.442 kb on + strand, within sctC at 661.442 kb on + strand, within sctC at 661.610 kb on + strand, within sctC at 661.626 kb on - strand, within sctC at 661.723 kb on - strand, within sctC at 661.736 kb on + strand, within sctC at 661.750 kb on + strand, within sctC at 661.793 kb on + strand, within sctC at 661.793 kb on + strand, within sctC at 661.794 kb on - strand, within sctC at 661.796 kb on + strand, within sctC at 661.797 kb on - strand, within sctC at 661.918 kb on + strand, within sctC at 661.918 kb on + strand, within sctC at 661.919 kb on - strand, within sctC at 661.919 kb on - strand, within sctC at 661.919 kb on - strand, within sctC at 661.945 kb on - strand, within sctC at 662.049 kb on - strand at 662.049 kb on - strand at 662.075 kb on + strand at 662.106 kb on + strand at 662.107 kb on - strand at 662.107 kb on - strand at 662.107 kb on - strand at 662.108 kb on + strand at 662.109 kb on - strand at 662.109 kb on - strand at 662.303 kb on + strand, within LU632_RS03245 at 662.303 kb on + strand, within LU632_RS03245 at 662.375 kb on + strand at 662.483 kb on + strand, within LU632_RS03250 at 662.557 kb on + strand, within LU632_RS03250 at 662.586 kb on + strand, within LU632_RS03250 at 662.606 kb on + strand, within LU632_RS03250 at 662.890 kb on - strand at 662.905 kb on - strand at 662.909 kb on + strand at 662.924 kb on + strand at 662.942 kb on + strand at 662.943 kb on - strand at 662.975 kb on + strand at 662.975 kb on + strand at 662.976 kb on - strand at 662.976 kb on - strand at 662.979 kb on - strand at 662.979 kb on - strand at 662.979 kb on - strand at 663.017 kb on + strand at 663.017 kb on + strand at 663.018 kb on - strand at 663.059 kb on + strand, within LU632_RS03255 at 663.059 kb on + strand, within LU632_RS03255 at 663.065 kb on + strand, within LU632_RS03255 at 663.247 kb on + strand, within LU632_RS03255 at 663.248 kb on - strand, within LU632_RS03255 at 663.256 kb on + strand, within LU632_RS03255 at 663.256 kb on + strand, within LU632_RS03255 at 663.328 kb on + strand, within LU632_RS03255
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Glucose remove 661,200 - sctC LU632_RS03240 0.52 +1.0 661,250 + sctC LU632_RS03240 0.55 -0.3 661,251 - sctC LU632_RS03240 0.55 +0.2 661,251 - sctC LU632_RS03240 0.55 -1.5 661,320 + sctC LU632_RS03240 0.58 -0.4 661,321 - sctC LU632_RS03240 0.58 +2.2 661,326 - sctC LU632_RS03240 0.58 -0.4 661,442 + sctC LU632_RS03240 0.64 -0.2 661,442 + sctC LU632_RS03240 0.64 -1.0 661,610 + sctC LU632_RS03240 0.72 +0.3 661,626 - sctC LU632_RS03240 0.73 +0.3 661,723 - sctC LU632_RS03240 0.78 +0.5 661,736 + sctC LU632_RS03240 0.79 -0.0 661,750 + sctC LU632_RS03240 0.79 +1.0 661,793 + sctC LU632_RS03240 0.81 +1.0 661,793 + sctC LU632_RS03240 0.81 +0.5 661,794 - sctC LU632_RS03240 0.81 +1.4 661,796 + sctC LU632_RS03240 0.82 -0.0 661,797 - sctC LU632_RS03240 0.82 +0.1 661,918 + sctC LU632_RS03240 0.88 +0.8 661,918 + sctC LU632_RS03240 0.88 -1.8 661,919 - sctC LU632_RS03240 0.88 -0.9 661,919 - sctC LU632_RS03240 0.88 -0.7 661,919 - sctC LU632_RS03240 0.88 +0.1 661,945 - sctC LU632_RS03240 0.89 -1.2 662,049 - -0.4 662,049 - -0.0 662,075 + +0.7 662,106 + -0.1 662,107 - -0.3 662,107 - -0.3 662,107 - -0.0 662,108 + +0.6 662,109 - +1.0 662,109 - +0.2 662,303 + LU632_RS03245 0.62 -0.1 662,303 + LU632_RS03245 0.62 -0.0 662,375 + -0.2 662,483 + LU632_RS03250 0.32 +0.2 662,557 + LU632_RS03250 0.53 +0.6 662,586 + LU632_RS03250 0.62 -1.4 662,606 + LU632_RS03250 0.67 -0.2 662,890 - -0.5 662,905 - +0.6 662,909 + -0.2 662,924 + -0.1 662,942 + +0.4 662,943 - -0.0 662,975 + -0.5 662,975 + -0.1 662,976 - -0.6 662,976 - -0.2 662,979 - +1.2 662,979 - -0.0 662,979 - +0.3 663,017 + -0.6 663,017 + -0.1 663,018 - +1.7 663,059 + LU632_RS03255 0.12 +0.7 663,059 + LU632_RS03255 0.12 +0.2 663,065 + LU632_RS03255 0.13 -0.4 663,247 + LU632_RS03255 0.28 -0.0 663,248 - LU632_RS03255 0.28 -0.5 663,256 + LU632_RS03255 0.28 +0.2 663,256 + LU632_RS03255 0.28 -0.4 663,328 + LU632_RS03255 0.34 +1.0
Or see this region's nucleotide sequence