Experiment: HBM-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS03200 and LU632_RS03205 are separated by 240 nucleotides LU632_RS03205 and LU632_RS03210 are separated by 67 nucleotides LU632_RS03210 and sctJ are separated by 5 nucleotides sctJ and LU632_RS03220 overlap by 8 nucleotides
LU632_RS03200: LU632_RS03200 - sigma-54 dependent transcriptional regulator, at 655,613 to 656,590
_RS03200
LU632_RS03205: LU632_RS03205 - hypothetical protein, at 656,831 to 657,034
_RS03205
LU632_RS03210: LU632_RS03210 - EscI/YscI/HrpB family type III secretion system inner rod protein, at 657,102 to 657,467
_RS03210
LU632_RS03215: sctJ - type III secretion inner membrane ring lipoprotein SctJ, at 657,473 to 658,249
sctJ
LU632_RS03220: LU632_RS03220 - hypothetical protein, at 658,242 to 658,832
_RS03220
Position (kb) 657
658 Strain fitness (log2 ratio) -2
-1
0
1
2 at 656.153 kb on + strand, within LU632_RS03200 at 656.153 kb on + strand, within LU632_RS03200 at 656.153 kb on + strand, within LU632_RS03200 at 656.165 kb on - strand, within LU632_RS03200 at 656.165 kb on - strand, within LU632_RS03200 at 656.215 kb on + strand, within LU632_RS03200 at 656.215 kb on + strand, within LU632_RS03200 at 656.216 kb on - strand, within LU632_RS03200 at 656.230 kb on + strand, within LU632_RS03200 at 656.314 kb on + strand, within LU632_RS03200 at 656.314 kb on + strand, within LU632_RS03200 at 656.559 kb on + strand at 656.559 kb on + strand at 656.560 kb on - strand at 656.578 kb on + strand at 656.578 kb on + strand at 656.578 kb on + strand at 656.579 kb on - strand at 656.579 kb on - strand at 656.671 kb on + strand at 656.672 kb on - strand at 656.672 kb on - strand at 656.767 kb on + strand at 656.767 kb on + strand at 656.767 kb on + strand at 656.768 kb on - strand at 656.770 kb on + strand at 656.772 kb on + strand at 656.805 kb on + strand at 656.862 kb on + strand, within LU632_RS03205 at 656.950 kb on + strand, within LU632_RS03205 at 656.951 kb on - strand, within LU632_RS03205 at 656.961 kb on + strand, within LU632_RS03205 at 656.962 kb on - strand, within LU632_RS03205 at 656.962 kb on - strand, within LU632_RS03205 at 656.962 kb on - strand, within LU632_RS03205 at 656.962 kb on - strand, within LU632_RS03205 at 657.017 kb on - strand at 657.152 kb on + strand, within LU632_RS03210 at 657.211 kb on + strand, within LU632_RS03210 at 657.269 kb on + strand, within LU632_RS03210 at 657.478 kb on + strand at 657.479 kb on - strand at 657.504 kb on + strand at 657.504 kb on + strand at 657.610 kb on + strand, within sctJ at 657.684 kb on + strand, within sctJ at 657.684 kb on + strand, within sctJ at 657.685 kb on - strand, within sctJ at 657.688 kb on + strand, within sctJ at 657.689 kb on - strand, within sctJ at 657.689 kb on - strand, within sctJ at 657.728 kb on - strand, within sctJ at 657.797 kb on + strand, within sctJ at 657.797 kb on + strand, within sctJ at 657.798 kb on - strand, within sctJ at 657.798 kb on - strand, within sctJ at 657.928 kb on + strand, within sctJ at 657.931 kb on + strand, within sctJ at 657.931 kb on + strand, within sctJ at 657.932 kb on - strand, within sctJ at 657.973 kb on + strand, within sctJ at 657.974 kb on - strand, within sctJ at 658.061 kb on + strand, within sctJ at 658.062 kb on - strand, within sctJ at 658.099 kb on + strand, within sctJ at 658.099 kb on + strand, within sctJ at 658.099 kb on + strand, within sctJ at 658.100 kb on - strand, within sctJ at 658.100 kb on - strand, within sctJ at 658.148 kb on - strand, within sctJ at 658.182 kb on + strand at 658.183 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Glucose remove 656,153 + LU632_RS03200 0.55 +0.5 656,153 + LU632_RS03200 0.55 -0.4 656,153 + LU632_RS03200 0.55 -0.1 656,165 - LU632_RS03200 0.56 -0.4 656,165 - LU632_RS03200 0.56 -0.0 656,215 + LU632_RS03200 0.62 +1.7 656,215 + LU632_RS03200 0.62 +0.1 656,216 - LU632_RS03200 0.62 +0.5 656,230 + LU632_RS03200 0.63 -0.4 656,314 + LU632_RS03200 0.72 -1.0 656,314 + LU632_RS03200 0.72 +0.7 656,559 + +0.5 656,559 + +0.5 656,560 - +1.0 656,578 + -2.0 656,578 + +0.4 656,578 + +0.5 656,579 - +0.5 656,579 - +0.2 656,671 + -0.1 656,672 - -0.0 656,672 - +0.4 656,767 + -1.6 656,767 + +0.4 656,767 + -0.3 656,768 - -0.2 656,770 + -1.5 656,772 + -0.2 656,805 + -0.8 656,862 + LU632_RS03205 0.15 +0.4 656,950 + LU632_RS03205 0.58 +2.0 656,951 - LU632_RS03205 0.59 -0.8 656,961 + LU632_RS03205 0.64 -0.1 656,962 - LU632_RS03205 0.64 +0.2 656,962 - LU632_RS03205 0.64 -1.4 656,962 - LU632_RS03205 0.64 +1.0 656,962 - LU632_RS03205 0.64 +0.3 657,017 - -0.1 657,152 + LU632_RS03210 0.14 -0.9 657,211 + LU632_RS03210 0.30 +0.3 657,269 + LU632_RS03210 0.46 +0.0 657,478 + +0.1 657,479 - +0.1 657,504 + +0.3 657,504 + -0.4 657,610 + sctJ LU632_RS03215 0.18 -0.2 657,684 + sctJ LU632_RS03215 0.27 -0.4 657,684 + sctJ LU632_RS03215 0.27 +0.3 657,685 - sctJ LU632_RS03215 0.27 +0.4 657,688 + sctJ LU632_RS03215 0.28 -1.6 657,689 - sctJ LU632_RS03215 0.28 +0.3 657,689 - sctJ LU632_RS03215 0.28 -0.2 657,728 - sctJ LU632_RS03215 0.33 -0.5 657,797 + sctJ LU632_RS03215 0.42 -1.0 657,797 + sctJ LU632_RS03215 0.42 +0.2 657,798 - sctJ LU632_RS03215 0.42 -0.6 657,798 - sctJ LU632_RS03215 0.42 +0.2 657,928 + sctJ LU632_RS03215 0.59 -0.3 657,931 + sctJ LU632_RS03215 0.59 +1.4 657,931 + sctJ LU632_RS03215 0.59 +0.2 657,932 - sctJ LU632_RS03215 0.59 +0.2 657,973 + sctJ LU632_RS03215 0.64 -0.1 657,974 - sctJ LU632_RS03215 0.64 -0.8 658,061 + sctJ LU632_RS03215 0.76 -0.3 658,062 - sctJ LU632_RS03215 0.76 -0.0 658,099 + sctJ LU632_RS03215 0.81 +0.6 658,099 + sctJ LU632_RS03215 0.81 +0.6 658,099 + sctJ LU632_RS03215 0.81 +1.0 658,100 - sctJ LU632_RS03215 0.81 +0.0 658,100 - sctJ LU632_RS03215 0.81 -0.0 658,148 - sctJ LU632_RS03215 0.87 -0.3 658,182 + +1.0 658,183 - -0.4
Or see this region's nucleotide sequence