Experiment: HBM-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS02345 and LU632_RS02350 are separated by 325 nucleotides LU632_RS02350 and iolB are separated by 18 nucleotides iolB and LU632_RS02360 are separated by 83 nucleotides
LU632_RS02345: LU632_RS02345 - TIM barrel protein, at 460,689 to 461,486
_RS02345
LU632_RS02350: LU632_RS02350 - CoA-acylating methylmalonate-semialdehyde dehydrogenase, at 461,812 to 463,317
_RS02350
LU632_RS02355: iolB - 5-deoxy-glucuronate isomerase, at 463,336 to 464,145
iolB
LU632_RS02360: LU632_RS02360 - MurR/RpiR family transcriptional regulator, at 464,229 to 465,083
_RS02360
Position (kb)
461
462
463
464 Strain fitness (log2 ratio)
-1
0
1
2 at 460.865 kb on + strand, within LU632_RS02345 at 460.866 kb on - strand, within LU632_RS02345 at 460.866 kb on - strand, within LU632_RS02345 at 460.866 kb on - strand, within LU632_RS02345 at 460.947 kb on - strand, within LU632_RS02345 at 460.950 kb on + strand, within LU632_RS02345 at 460.950 kb on + strand, within LU632_RS02345 at 460.951 kb on - strand, within LU632_RS02345 at 460.982 kb on - strand, within LU632_RS02345 at 461.016 kb on - strand, within LU632_RS02345 at 461.041 kb on - strand, within LU632_RS02345 at 461.041 kb on - strand, within LU632_RS02345 at 461.041 kb on - strand, within LU632_RS02345 at 461.041 kb on - strand, within LU632_RS02345 at 461.041 kb on - strand, within LU632_RS02345 at 461.109 kb on + strand, within LU632_RS02345 at 461.288 kb on + strand, within LU632_RS02345 at 461.289 kb on - strand, within LU632_RS02345 at 461.364 kb on - strand, within LU632_RS02345 at 461.384 kb on + strand, within LU632_RS02345 at 461.397 kb on + strand, within LU632_RS02345 at 461.397 kb on + strand, within LU632_RS02345 at 461.398 kb on - strand, within LU632_RS02345 at 461.415 kb on - strand at 461.818 kb on - strand at 462.014 kb on + strand, within LU632_RS02350 at 462.015 kb on - strand, within LU632_RS02350 at 462.015 kb on - strand, within LU632_RS02350 at 462.015 kb on - strand, within LU632_RS02350 at 462.070 kb on - strand, within LU632_RS02350 at 462.070 kb on - strand, within LU632_RS02350 at 462.088 kb on - strand, within LU632_RS02350 at 462.211 kb on + strand, within LU632_RS02350 at 462.212 kb on - strand, within LU632_RS02350 at 462.250 kb on - strand, within LU632_RS02350 at 462.250 kb on - strand, within LU632_RS02350 at 462.424 kb on - strand, within LU632_RS02350 at 462.448 kb on + strand, within LU632_RS02350 at 462.448 kb on + strand, within LU632_RS02350 at 462.482 kb on + strand, within LU632_RS02350 at 462.513 kb on + strand, within LU632_RS02350 at 462.690 kb on + strand, within LU632_RS02350 at 462.738 kb on - strand, within LU632_RS02350 at 462.866 kb on + strand, within LU632_RS02350 at 462.867 kb on - strand, within LU632_RS02350 at 462.877 kb on - strand, within LU632_RS02350 at 462.908 kb on - strand, within LU632_RS02350 at 462.910 kb on + strand, within LU632_RS02350 at 462.911 kb on - strand, within LU632_RS02350 at 462.911 kb on - strand, within LU632_RS02350 at 462.911 kb on - strand, within LU632_RS02350 at 463.009 kb on + strand, within LU632_RS02350 at 463.010 kb on - strand, within LU632_RS02350 at 463.061 kb on + strand, within LU632_RS02350 at 463.062 kb on - strand, within LU632_RS02350 at 463.074 kb on + strand, within LU632_RS02350 at 463.100 kb on + strand, within LU632_RS02350 at 463.100 kb on + strand, within LU632_RS02350 at 463.101 kb on - strand, within LU632_RS02350 at 463.372 kb on - strand at 463.616 kb on + strand, within iolB at 463.617 kb on - strand, within iolB at 463.667 kb on + strand, within iolB at 463.668 kb on - strand, within iolB at 463.704 kb on - strand, within iolB at 463.704 kb on - strand, within iolB at 463.709 kb on + strand, within iolB at 463.710 kb on - strand, within iolB at 463.732 kb on - strand, within iolB at 463.746 kb on - strand, within iolB at 463.753 kb on - strand, within iolB at 463.753 kb on - strand, within iolB at 463.839 kb on + strand, within iolB at 463.888 kb on + strand, within iolB at 463.888 kb on + strand, within iolB at 463.888 kb on + strand, within iolB at 463.889 kb on - strand, within iolB at 463.889 kb on - strand, within iolB at 463.892 kb on - strand, within iolB at 463.892 kb on - strand, within iolB at 463.937 kb on - strand, within iolB at 463.992 kb on + strand, within iolB at 464.044 kb on + strand, within iolB at 464.045 kb on - strand, within iolB at 464.045 kb on - strand, within iolB at 464.045 kb on - strand, within iolB at 464.045 kb on - strand, within iolB at 464.047 kb on + strand, within iolB at 464.047 kb on + strand, within iolB at 464.048 kb on - strand, within iolB at 464.048 kb on - strand, within iolB at 464.048 kb on - strand, within iolB at 464.076 kb on + strand at 464.077 kb on - strand at 464.115 kb on - strand at 464.125 kb on - strand at 464.125 kb on - strand at 464.129 kb on - strand at 464.129 kb on - strand at 464.184 kb on + strand at 464.230 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Glucose remove 460,865 + LU632_RS02345 0.22 +1.0 460,866 - LU632_RS02345 0.22 +0.2 460,866 - LU632_RS02345 0.22 +2.0 460,866 - LU632_RS02345 0.22 -0.0 460,947 - LU632_RS02345 0.32 +0.0 460,950 + LU632_RS02345 0.33 +0.7 460,950 + LU632_RS02345 0.33 -0.3 460,951 - LU632_RS02345 0.33 +0.7 460,982 - LU632_RS02345 0.37 -0.1 461,016 - LU632_RS02345 0.41 -0.2 461,041 - LU632_RS02345 0.44 -0.2 461,041 - LU632_RS02345 0.44 +0.0 461,041 - LU632_RS02345 0.44 +0.6 461,041 - LU632_RS02345 0.44 -0.2 461,041 - LU632_RS02345 0.44 +1.5 461,109 + LU632_RS02345 0.53 -0.0 461,288 + LU632_RS02345 0.75 -0.6 461,289 - LU632_RS02345 0.75 -1.5 461,364 - LU632_RS02345 0.85 -0.3 461,384 + LU632_RS02345 0.87 +0.3 461,397 + LU632_RS02345 0.89 -0.1 461,397 + LU632_RS02345 0.89 -0.1 461,398 - LU632_RS02345 0.89 +0.7 461,415 - +0.3 461,818 - -0.2 462,014 + LU632_RS02350 0.13 +1.0 462,015 - LU632_RS02350 0.13 -1.0 462,015 - LU632_RS02350 0.13 -1.1 462,015 - LU632_RS02350 0.13 -1.1 462,070 - LU632_RS02350 0.17 -0.1 462,070 - LU632_RS02350 0.17 +0.9 462,088 - LU632_RS02350 0.18 +1.0 462,211 + LU632_RS02350 0.26 +0.6 462,212 - LU632_RS02350 0.27 -0.2 462,250 - LU632_RS02350 0.29 +0.3 462,250 - LU632_RS02350 0.29 +0.5 462,424 - LU632_RS02350 0.41 +0.5 462,448 + LU632_RS02350 0.42 -0.2 462,448 + LU632_RS02350 0.42 -0.3 462,482 + LU632_RS02350 0.44 +0.5 462,513 + LU632_RS02350 0.47 +1.0 462,690 + LU632_RS02350 0.58 +0.0 462,738 - LU632_RS02350 0.61 -0.3 462,866 + LU632_RS02350 0.70 +0.3 462,867 - LU632_RS02350 0.70 +0.5 462,877 - LU632_RS02350 0.71 -1.1 462,908 - LU632_RS02350 0.73 +0.8 462,910 + LU632_RS02350 0.73 -0.5 462,911 - LU632_RS02350 0.73 +0.5 462,911 - LU632_RS02350 0.73 +0.2 462,911 - LU632_RS02350 0.73 +0.4 463,009 + LU632_RS02350 0.79 -0.5 463,010 - LU632_RS02350 0.80 +0.2 463,061 + LU632_RS02350 0.83 +0.6 463,062 - LU632_RS02350 0.83 +0.3 463,074 + LU632_RS02350 0.84 -0.4 463,100 + LU632_RS02350 0.86 -0.5 463,100 + LU632_RS02350 0.86 -0.4 463,101 - LU632_RS02350 0.86 +1.0 463,372 - -0.4 463,616 + iolB LU632_RS02355 0.35 -0.4 463,617 - iolB LU632_RS02355 0.35 +0.3 463,667 + iolB LU632_RS02355 0.41 +0.3 463,668 - iolB LU632_RS02355 0.41 +0.5 463,704 - iolB LU632_RS02355 0.45 +0.2 463,704 - iolB LU632_RS02355 0.45 +0.2 463,709 + iolB LU632_RS02355 0.46 -0.7 463,710 - iolB LU632_RS02355 0.46 +0.7 463,732 - iolB LU632_RS02355 0.49 +0.3 463,746 - iolB LU632_RS02355 0.51 -0.4 463,753 - iolB LU632_RS02355 0.51 -0.5 463,753 - iolB LU632_RS02355 0.51 +0.4 463,839 + iolB LU632_RS02355 0.62 -0.5 463,888 + iolB LU632_RS02355 0.68 +0.5 463,888 + iolB LU632_RS02355 0.68 -0.4 463,888 + iolB LU632_RS02355 0.68 +1.8 463,889 - iolB LU632_RS02355 0.68 -1.0 463,889 - iolB LU632_RS02355 0.68 -0.1 463,892 - iolB LU632_RS02355 0.69 +0.0 463,892 - iolB LU632_RS02355 0.69 +1.3 463,937 - iolB LU632_RS02355 0.74 -0.3 463,992 + iolB LU632_RS02355 0.81 -0.4 464,044 + iolB LU632_RS02355 0.87 +0.3 464,045 - iolB LU632_RS02355 0.88 -0.8 464,045 - iolB LU632_RS02355 0.88 -0.7 464,045 - iolB LU632_RS02355 0.88 -0.0 464,045 - iolB LU632_RS02355 0.88 -1.0 464,047 + iolB LU632_RS02355 0.88 -1.0 464,047 + iolB LU632_RS02355 0.88 -0.1 464,048 - iolB LU632_RS02355 0.88 +0.1 464,048 - iolB LU632_RS02355 0.88 +0.5 464,048 - iolB LU632_RS02355 0.88 +0.2 464,076 + -0.5 464,077 - +0.3 464,115 - -0.8 464,125 - -0.2 464,125 - -1.6 464,129 - +0.0 464,129 - +0.6 464,184 + +1.6 464,230 + +1.5
Or see this region's nucleotide sequence