Experiment: HBM-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS00605 and LU632_RS00610 are separated by 470 nucleotides LU632_RS00610 and kduD are separated by 42 nucleotides kduD and LU632_RS00620 are separated by 13 nucleotides
LU632_RS00605: LU632_RS00605 - IS630 family transposase, at 123,642 to 124,624
_RS00605
LU632_RS00610: LU632_RS00610 - RpiB/LacA/LacB family sugar-phosphate isomerase, at 125,095 to 125,736
_RS00610
LU632_RS00615: kduD - 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase KduD, at 125,779 to 126,540
kduD
LU632_RS00620: LU632_RS00620 - glycoside hydrolase family 88 protein, at 126,554 to 127,693
_RS00620
Position (kb)
125
126 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 124.096 kb on - strand, within LU632_RS00605 at 124.118 kb on - strand, within LU632_RS00605 at 124.139 kb on + strand, within LU632_RS00605 at 124.219 kb on - strand, within LU632_RS00605 at 124.219 kb on - strand, within LU632_RS00605 at 124.245 kb on - strand, within LU632_RS00605 at 124.334 kb on - strand, within LU632_RS00605 at 124.622 kb on + strand at 124.667 kb on - strand at 124.845 kb on + strand at 124.845 kb on + strand at 124.846 kb on - strand at 124.849 kb on + strand at 124.869 kb on - strand at 124.950 kb on + strand at 125.040 kb on + strand at 125.042 kb on + strand at 125.042 kb on + strand at 125.042 kb on + strand at 125.043 kb on - strand at 125.043 kb on - strand at 125.043 kb on - strand at 125.043 kb on - strand at 125.047 kb on + strand at 125.048 kb on - strand at 125.048 kb on - strand at 125.063 kb on - strand at 125.065 kb on + strand at 125.068 kb on + strand at 125.151 kb on + strand at 125.231 kb on + strand, within LU632_RS00610 at 125.232 kb on - strand, within LU632_RS00610 at 125.335 kb on - strand, within LU632_RS00610 at 125.354 kb on + strand, within LU632_RS00610 at 125.425 kb on - strand, within LU632_RS00610 at 125.460 kb on + strand, within LU632_RS00610 at 125.461 kb on - strand, within LU632_RS00610 at 125.488 kb on + strand, within LU632_RS00610 at 125.488 kb on + strand, within LU632_RS00610 at 125.489 kb on - strand, within LU632_RS00610 at 125.489 kb on - strand, within LU632_RS00610 at 125.489 kb on - strand, within LU632_RS00610 at 125.489 kb on - strand, within LU632_RS00610 at 125.490 kb on + strand, within LU632_RS00610 at 125.491 kb on - strand, within LU632_RS00610 at 125.491 kb on - strand, within LU632_RS00610 at 125.504 kb on - strand, within LU632_RS00610 at 125.533 kb on - strand, within LU632_RS00610 at 125.536 kb on - strand, within LU632_RS00610 at 125.578 kb on - strand, within LU632_RS00610 at 125.594 kb on + strand, within LU632_RS00610 at 125.595 kb on - strand, within LU632_RS00610 at 125.634 kb on + strand, within LU632_RS00610 at 125.634 kb on + strand, within LU632_RS00610 at 125.635 kb on - strand, within LU632_RS00610 at 125.635 kb on - strand, within LU632_RS00610 at 125.635 kb on - strand, within LU632_RS00610 at 125.639 kb on - strand, within LU632_RS00610 at 125.672 kb on - strand at 125.825 kb on - strand at 125.827 kb on - strand at 125.827 kb on - strand at 125.827 kb on - strand at 125.827 kb on - strand at 125.827 kb on - strand at 125.828 kb on + strand at 125.828 kb on + strand at 125.828 kb on + strand at 125.829 kb on - strand at 125.829 kb on - strand at 126.000 kb on + strand, within kduD at 126.000 kb on + strand, within kduD at 126.000 kb on + strand, within kduD at 126.001 kb on - strand, within kduD at 126.005 kb on + strand, within kduD at 126.006 kb on - strand, within kduD at 126.578 kb on + strand at 126.622 kb on + strand at 126.623 kb on - strand at 126.706 kb on + strand, within LU632_RS00620 at 126.706 kb on + strand, within LU632_RS00620 at 126.706 kb on + strand, within LU632_RS00620 at 126.706 kb on + strand, within LU632_RS00620 at 126.707 kb on - strand, within LU632_RS00620 at 126.707 kb on - strand, within LU632_RS00620 at 126.707 kb on - strand, within LU632_RS00620
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Glucose remove 124,096 - LU632_RS00605 0.46 +0.4 124,118 - LU632_RS00605 0.48 +0.5 124,139 + LU632_RS00605 0.51 +0.3 124,219 - LU632_RS00605 0.59 +1.4 124,219 - LU632_RS00605 0.59 +0.0 124,245 - LU632_RS00605 0.61 +0.4 124,334 - LU632_RS00605 0.70 -0.3 124,622 + +0.2 124,667 - -0.9 124,845 + -1.5 124,845 + -0.1 124,846 - -0.6 124,849 + -0.3 124,869 - -0.4 124,950 + -0.7 125,040 + -0.0 125,042 + +0.0 125,042 + -0.1 125,042 + -0.4 125,043 - +0.3 125,043 - +0.9 125,043 - -0.8 125,043 - +1.1 125,047 + +0.1 125,048 - -0.1 125,048 - -0.1 125,063 - -1.0 125,065 + +0.0 125,068 + +0.1 125,151 + +0.0 125,231 + LU632_RS00610 0.21 -0.1 125,232 - LU632_RS00610 0.21 +0.4 125,335 - LU632_RS00610 0.37 -0.0 125,354 + LU632_RS00610 0.40 +0.1 125,425 - LU632_RS00610 0.51 +0.1 125,460 + LU632_RS00610 0.57 +0.3 125,461 - LU632_RS00610 0.57 +0.8 125,488 + LU632_RS00610 0.61 -1.8 125,488 + LU632_RS00610 0.61 -0.1 125,489 - LU632_RS00610 0.61 +0.2 125,489 - LU632_RS00610 0.61 +0.1 125,489 - LU632_RS00610 0.61 -0.7 125,489 - LU632_RS00610 0.61 -0.0 125,490 + LU632_RS00610 0.62 -0.8 125,491 - LU632_RS00610 0.62 +0.3 125,491 - LU632_RS00610 0.62 +0.1 125,504 - LU632_RS00610 0.64 +0.5 125,533 - LU632_RS00610 0.68 -1.4 125,536 - LU632_RS00610 0.69 -0.0 125,578 - LU632_RS00610 0.75 +1.3 125,594 + LU632_RS00610 0.78 +0.2 125,595 - LU632_RS00610 0.78 -0.0 125,634 + LU632_RS00610 0.84 +0.8 125,634 + LU632_RS00610 0.84 +0.6 125,635 - LU632_RS00610 0.84 +0.0 125,635 - LU632_RS00610 0.84 -0.4 125,635 - LU632_RS00610 0.84 -0.4 125,639 - LU632_RS00610 0.85 +0.1 125,672 - -0.5 125,825 - -0.7 125,827 - +1.6 125,827 - +0.1 125,827 - +0.6 125,827 - -0.2 125,827 - -0.3 125,828 + -0.3 125,828 + +0.1 125,828 + +0.5 125,829 - -1.1 125,829 - -0.1 126,000 + kduD LU632_RS00615 0.29 -0.4 126,000 + kduD LU632_RS00615 0.29 -0.4 126,000 + kduD LU632_RS00615 0.29 +0.5 126,001 - kduD LU632_RS00615 0.29 -0.4 126,005 + kduD LU632_RS00615 0.30 -0.6 126,006 - kduD LU632_RS00615 0.30 -0.1 126,578 + -2.2 126,622 + -0.1 126,623 - +1.1 126,706 + LU632_RS00620 0.13 +0.8 126,706 + LU632_RS00620 0.13 -3.4 126,706 + LU632_RS00620 0.13 +0.9 126,706 + LU632_RS00620 0.13 +2.9 126,707 - LU632_RS00620 0.13 -0.3 126,707 - LU632_RS00620 0.13 +0.0 126,707 - LU632_RS00620 0.13 +1.7
Or see this region's nucleotide sequence