Strain Fitness in Escherichia coli BL21 around ECD_03065

Experiment: Control_TnA

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_03063 and ECD_03064 overlap by 4 nucleotidesECD_03064 and ECD_03065 overlap by 32 nucleotidesECD_03065 and ECD_03066 are separated by 6 nucleotidesECD_03066 and ECD_03067 are separated by 47 nucleotides ECD_03063: ECD_03063 - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, at 3,210,773 to 3,211,339 _03063 ECD_03064: ECD_03064 - periplasmic membrane-anchored LPS-binding protein; LPS export protein, at 3,211,336 to 3,211,911 _03064 ECD_03065: ECD_03065 - lipopolysaccharide export ABC transporter periplasmic binding protein; Lipid A binding protein; LPS export and assembly protein, at 3,211,880 to 3,212,437 _03065 ECD_03066: ECD_03066 - lipopolysaccharide export ABC transporter ATPase, at 3,212,444 to 3,213,169 _03066 ECD_03067: ECD_03067 - RNA polymerase, sigma 54 (sigma N) factor, at 3,213,217 to 3,214,650 _03067 Position (kb) 3211 3212 3213Strain fitness (log2 ratio) -2 -1 0 1at 3210.899 kb on + strand, within ECD_03063at 3210.899 kb on + strand, within ECD_03063at 3210.899 kb on + strand, within ECD_03063at 3210.899 kb on + strand, within ECD_03063at 3211.058 kb on + strand, within ECD_03063at 3211.136 kb on + strand, within ECD_03063at 3211.287 kb on + strandat 3211.287 kb on + strandat 3211.287 kb on + strandat 3211.287 kb on + strandat 3211.287 kb on + strandat 3211.764 kb on + strand, within ECD_03064at 3211.764 kb on + strand, within ECD_03064at 3211.787 kb on + strand, within ECD_03064at 3211.789 kb on + strand, within ECD_03064at 3213.193 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_TnA
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3,210,899 + ECD_03063 0.22 -0.8
3,210,899 + ECD_03063 0.22 -1.3
3,210,899 + ECD_03063 0.22 -0.9
3,210,899 + ECD_03063 0.22 -1.5
3,211,058 + ECD_03063 0.50 +0.3
3,211,136 + ECD_03063 0.64 +0.3
3,211,287 + -0.5
3,211,287 + -0.6
3,211,287 + -2.3
3,211,287 + -1.4
3,211,287 + -1.3
3,211,764 + ECD_03064 0.74 +0.0
3,211,764 + ECD_03064 0.74 -0.0
3,211,787 + ECD_03064 0.78 +0.1
3,211,789 + ECD_03064 0.79 -0.1
3,213,193 + +1.5

Or see this region's nucleotide sequence