Experiment: HBM-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glnG and glnL are separated by 7 nucleotides glnL and glnA are separated by 186 nucleotides
LU632_RS22135: glnG - nitrogen regulation protein NR(I), at 4,227,459 to 4,228,868
glnG
LU632_RS22140: glnL - nitrogen regulation protein NR(II), at 4,228,876 to 4,229,925
glnL
LU632_RS22145: glnA - glutamate--ammonia ligase, at 4,230,112 to 4,231,521
glnA
Position (kb) 4228
4229
4230 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4227.917 kb on + strand, within glnG at 4227.966 kb on + strand, within glnG at 4228.032 kb on - strand, within glnG at 4228.201 kb on - strand, within glnG at 4228.280 kb on + strand, within glnG at 4228.341 kb on + strand, within glnG at 4228.449 kb on - strand, within glnG at 4228.568 kb on - strand, within glnG at 4228.628 kb on + strand, within glnG at 4228.670 kb on + strand, within glnG at 4228.671 kb on - strand, within glnG at 4228.888 kb on - strand at 4228.936 kb on + strand at 4229.022 kb on - strand, within glnL at 4229.039 kb on - strand, within glnL at 4229.145 kb on + strand, within glnL at 4229.145 kb on + strand, within glnL at 4229.145 kb on + strand, within glnL at 4229.332 kb on - strand, within glnL at 4229.427 kb on - strand, within glnL at 4229.427 kb on - strand, within glnL at 4229.553 kb on + strand, within glnL at 4229.553 kb on + strand, within glnL at 4229.554 kb on - strand, within glnL at 4229.554 kb on - strand, within glnL at 4229.618 kb on - strand, within glnL at 4229.652 kb on + strand, within glnL at 4229.652 kb on + strand, within glnL at 4229.652 kb on + strand, within glnL at 4229.652 kb on + strand, within glnL at 4229.652 kb on + strand, within glnL at 4229.653 kb on - strand, within glnL at 4229.653 kb on - strand, within glnL at 4229.739 kb on + strand, within glnL at 4229.739 kb on + strand, within glnL at 4229.740 kb on - strand, within glnL at 4229.867 kb on - strand at 4229.868 kb on + strand at 4229.868 kb on + strand at 4229.869 kb on - strand at 4229.869 kb on - strand at 4229.869 kb on - strand at 4229.869 kb on - strand at 4229.869 kb on - strand at 4229.869 kb on - strand at 4229.869 kb on - strand at 4229.925 kb on + strand at 4230.062 kb on + strand at 4230.062 kb on + strand at 4230.063 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Glucose remove 4,227,917 + glnG LU632_RS22135 0.32 -2.1 4,227,966 + glnG LU632_RS22135 0.36 -2.2 4,228,032 - glnG LU632_RS22135 0.41 -1.4 4,228,201 - glnG LU632_RS22135 0.53 -1.8 4,228,280 + glnG LU632_RS22135 0.58 -2.2 4,228,341 + glnG LU632_RS22135 0.63 -2.1 4,228,449 - glnG LU632_RS22135 0.70 -2.4 4,228,568 - glnG LU632_RS22135 0.79 -1.2 4,228,628 + glnG LU632_RS22135 0.83 -1.9 4,228,670 + glnG LU632_RS22135 0.86 -2.4 4,228,671 - glnG LU632_RS22135 0.86 -1.4 4,228,888 - +0.8 4,228,936 + -0.8 4,229,022 - glnL LU632_RS22140 0.14 -2.5 4,229,039 - glnL LU632_RS22140 0.16 -0.4 4,229,145 + glnL LU632_RS22140 0.26 -2.3 4,229,145 + glnL LU632_RS22140 0.26 -1.8 4,229,145 + glnL LU632_RS22140 0.26 -1.2 4,229,332 - glnL LU632_RS22140 0.43 -1.7 4,229,427 - glnL LU632_RS22140 0.52 -1.9 4,229,427 - glnL LU632_RS22140 0.52 -2.4 4,229,553 + glnL LU632_RS22140 0.64 -2.0 4,229,553 + glnL LU632_RS22140 0.64 -2.1 4,229,554 - glnL LU632_RS22140 0.65 -0.2 4,229,554 - glnL LU632_RS22140 0.65 -2.1 4,229,618 - glnL LU632_RS22140 0.71 -3.0 4,229,652 + glnL LU632_RS22140 0.74 -1.8 4,229,652 + glnL LU632_RS22140 0.74 -2.9 4,229,652 + glnL LU632_RS22140 0.74 -1.8 4,229,652 + glnL LU632_RS22140 0.74 -2.6 4,229,652 + glnL LU632_RS22140 0.74 -2.0 4,229,653 - glnL LU632_RS22140 0.74 -2.0 4,229,653 - glnL LU632_RS22140 0.74 -2.0 4,229,739 + glnL LU632_RS22140 0.82 -2.5 4,229,739 + glnL LU632_RS22140 0.82 -0.8 4,229,740 - glnL LU632_RS22140 0.82 -2.4 4,229,867 - -2.3 4,229,868 + -1.8 4,229,868 + -1.9 4,229,869 - -1.5 4,229,869 - -2.5 4,229,869 - -2.4 4,229,869 - -1.0 4,229,869 - -1.5 4,229,869 - -0.8 4,229,869 - -2.0 4,229,925 + -1.5 4,230,062 + -1.3 4,230,062 + -0.5 4,230,063 - -1.8
Or see this region's nucleotide sequence