Experiment: HBM-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tnpA and tatE are separated by 167 nucleotides tatE and LU632_RS16420 are separated by 344 nucleotides LU632_RS16420 and LU632_RS16425 are separated by 158 nucleotides LU632_RS16425 and LU632_RS16430 are separated by 166 nucleotides
LU632_RS16410: tnpA - IS200/IS605 family transposase, at 3,107,359 to 3,107,844
tnpA
LU632_RS16415: tatE - twin-arginine translocase subunit TatE, at 3,108,012 to 3,108,218
tatE
LU632_RS16420: LU632_RS16420 - deaminated glutathione amidase, at 3,108,563 to 3,109,384
_RS16420
LU632_RS16425: LU632_RS16425 - GNAT family N-acetyltransferase, at 3,109,543 to 3,109,971
_RS16425
LU632_RS16430: LU632_RS16430 - IS1 family transposase, at 3,110,138 to 3,110,641
_RS16430
Position (kb)
3108
3109
3110 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3108.168 kb on + strand, within tatE at 3108.299 kb on - strand at 3108.321 kb on + strand at 3108.322 kb on - strand at 3108.322 kb on - strand at 3108.324 kb on + strand at 3108.492 kb on + strand at 3108.517 kb on + strand at 3108.517 kb on + strand at 3108.518 kb on - strand at 3108.518 kb on - strand at 3108.522 kb on - strand at 3108.522 kb on - strand at 3108.554 kb on - strand at 3108.658 kb on - strand, within LU632_RS16420 at 3108.751 kb on + strand, within LU632_RS16420 at 3108.752 kb on - strand, within LU632_RS16420 at 3108.754 kb on + strand, within LU632_RS16420 at 3108.754 kb on + strand, within LU632_RS16420 at 3108.760 kb on + strand, within LU632_RS16420 at 3108.796 kb on - strand, within LU632_RS16420 at 3109.030 kb on + strand, within LU632_RS16420 at 3109.033 kb on + strand, within LU632_RS16420 at 3109.033 kb on + strand, within LU632_RS16420 at 3109.033 kb on + strand, within LU632_RS16420 at 3109.044 kb on + strand, within LU632_RS16420 at 3109.052 kb on + strand, within LU632_RS16420 at 3109.052 kb on + strand, within LU632_RS16420 at 3109.052 kb on + strand, within LU632_RS16420 at 3109.053 kb on - strand, within LU632_RS16420 at 3109.135 kb on - strand, within LU632_RS16420 at 3109.135 kb on - strand, within LU632_RS16420 at 3109.243 kb on + strand, within LU632_RS16420 at 3109.271 kb on + strand, within LU632_RS16420 at 3109.272 kb on - strand, within LU632_RS16420 at 3109.275 kb on - strand, within LU632_RS16420 at 3109.277 kb on + strand, within LU632_RS16420 at 3109.278 kb on - strand, within LU632_RS16420 at 3109.280 kb on + strand, within LU632_RS16420 at 3109.398 kb on - strand at 3109.481 kb on + strand at 3109.490 kb on + strand at 3109.491 kb on - strand at 3109.502 kb on + strand at 3109.502 kb on + strand at 3109.561 kb on - strand at 3109.568 kb on + strand at 3109.569 kb on - strand at 3109.586 kb on + strand, within LU632_RS16425 at 3109.609 kb on - strand, within LU632_RS16425 at 3109.685 kb on + strand, within LU632_RS16425 at 3109.691 kb on - strand, within LU632_RS16425 at 3109.743 kb on - strand, within LU632_RS16425 at 3109.774 kb on + strand, within LU632_RS16425 at 3109.786 kb on - strand, within LU632_RS16425 at 3109.937 kb on + strand at 3109.938 kb on - strand at 3110.022 kb on + strand at 3110.023 kb on - strand at 3110.039 kb on - strand at 3110.101 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Glucose remove 3,108,168 + tatE LU632_RS16415 0.75 -0.3 3,108,299 - -0.7 3,108,321 + +0.4 3,108,322 - -0.4 3,108,322 - -0.3 3,108,324 + +0.4 3,108,492 + +0.7 3,108,517 + +0.6 3,108,517 + +0.9 3,108,518 - -0.1 3,108,518 - +0.4 3,108,522 - -0.6 3,108,522 - -0.5 3,108,554 - -0.1 3,108,658 - LU632_RS16420 0.12 +1.8 3,108,751 + LU632_RS16420 0.23 +0.4 3,108,752 - LU632_RS16420 0.23 +0.2 3,108,754 + LU632_RS16420 0.23 -1.8 3,108,754 + LU632_RS16420 0.23 -0.1 3,108,760 + LU632_RS16420 0.24 -0.1 3,108,796 - LU632_RS16420 0.28 -0.5 3,109,030 + LU632_RS16420 0.57 -0.6 3,109,033 + LU632_RS16420 0.57 +0.4 3,109,033 + LU632_RS16420 0.57 -0.5 3,109,033 + LU632_RS16420 0.57 +1.5 3,109,044 + LU632_RS16420 0.59 +0.7 3,109,052 + LU632_RS16420 0.59 +0.1 3,109,052 + LU632_RS16420 0.59 -0.4 3,109,052 + LU632_RS16420 0.59 +1.0 3,109,053 - LU632_RS16420 0.60 -0.1 3,109,135 - LU632_RS16420 0.70 +1.5 3,109,135 - LU632_RS16420 0.70 +0.6 3,109,243 + LU632_RS16420 0.83 +0.0 3,109,271 + LU632_RS16420 0.86 -0.4 3,109,272 - LU632_RS16420 0.86 -0.2 3,109,275 - LU632_RS16420 0.87 +0.6 3,109,277 + LU632_RS16420 0.87 +0.0 3,109,278 - LU632_RS16420 0.87 -0.3 3,109,280 + LU632_RS16420 0.87 +0.0 3,109,398 - +0.3 3,109,481 + -0.3 3,109,490 + -1.4 3,109,491 - -0.1 3,109,502 + +1.1 3,109,502 + -0.0 3,109,561 - +0.3 3,109,568 + +0.6 3,109,569 - -0.3 3,109,586 + LU632_RS16425 0.10 -0.2 3,109,609 - LU632_RS16425 0.15 -0.3 3,109,685 + LU632_RS16425 0.33 +0.1 3,109,691 - LU632_RS16425 0.34 -0.8 3,109,743 - LU632_RS16425 0.47 -1.7 3,109,774 + LU632_RS16425 0.54 -0.4 3,109,786 - LU632_RS16425 0.57 -0.1 3,109,937 + +0.4 3,109,938 - +0.7 3,110,022 + -0.1 3,110,023 - +0.0 3,110,039 - +0.2 3,110,101 - +0.1
Or see this region's nucleotide sequence