Experiment: HBM-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lnt and LU632_RS16330 are separated by 96 nucleotides LU632_RS16330 and leuS are separated by 212 nucleotides leuS and lptE are separated by 14 nucleotides lptE and holA overlap by 4 nucleotides
LU632_RS16325: lnt - apolipoprotein N-acyltransferase, at 3,088,102 to 3,089,634
lnt
LU632_RS16330: LU632_RS16330 - zinc ribbon-containing protein, at 3,089,731 to 3,090,213
_RS16330
LU632_RS16335: leuS - leucine--tRNA ligase, at 3,090,426 to 3,093,008
leuS
LU632_RS16340: lptE - LPS assembly lipoprotein LptE, at 3,093,023 to 3,093,619
lptE
LU632_RS16345: holA - DNA polymerase III subunit delta, at 3,093,616 to 3,094,647
holA
Position (kb)
3090
3091
3092
3093
3094 Strain fitness (log2 ratio)
-2
-1
0
1 at 3089.739 kb on + strand at 3089.804 kb on + strand, within LU632_RS16330 at 3089.937 kb on + strand, within LU632_RS16330 at 3089.938 kb on - strand, within LU632_RS16330 at 3090.105 kb on + strand, within LU632_RS16330 at 3090.106 kb on - strand, within LU632_RS16330 at 3090.106 kb on - strand, within LU632_RS16330 at 3090.106 kb on - strand, within LU632_RS16330 at 3090.106 kb on - strand, within LU632_RS16330 at 3090.106 kb on - strand, within LU632_RS16330 at 3090.139 kb on - strand, within LU632_RS16330 at 3090.157 kb on - strand, within LU632_RS16330 at 3090.182 kb on - strand at 3090.195 kb on + strand at 3090.195 kb on - strand at 3090.195 kb on - strand at 3090.215 kb on - strand at 3090.215 kb on - strand at 3090.215 kb on - strand at 3090.301 kb on + strand at 3090.371 kb on + strand at 3090.371 kb on + strand at 3090.371 kb on + strand at 3093.505 kb on + strand, within lptE at 3093.541 kb on + strand, within lptE
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Glucose remove 3,089,739 + +0.9 3,089,804 + LU632_RS16330 0.15 -0.1 3,089,937 + LU632_RS16330 0.43 +0.9 3,089,938 - LU632_RS16330 0.43 -0.1 3,090,105 + LU632_RS16330 0.77 -0.2 3,090,106 - LU632_RS16330 0.78 -1.1 3,090,106 - LU632_RS16330 0.78 -0.2 3,090,106 - LU632_RS16330 0.78 +0.6 3,090,106 - LU632_RS16330 0.78 -1.4 3,090,106 - LU632_RS16330 0.78 -0.3 3,090,139 - LU632_RS16330 0.84 +0.6 3,090,157 - LU632_RS16330 0.88 -0.5 3,090,182 - -1.0 3,090,195 + -0.0 3,090,195 - -0.8 3,090,195 - -1.8 3,090,215 - -0.8 3,090,215 - -0.6 3,090,215 - -0.1 3,090,301 + -0.8 3,090,371 + -0.8 3,090,371 + +0.1 3,090,371 + +0.4 3,093,505 + lptE LU632_RS16340 0.81 -0.6 3,093,541 + lptE LU632_RS16340 0.87 +0.6
Or see this region's nucleotide sequence