Experiment: HBM-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nagZ and thiK are separated by 9 nucleotides thiK and lpoB overlap by 41 nucleotides lpoB and LU632_RS14770 are separated by 41 nucleotides LU632_RS14770 and hinT are separated by 49 nucleotides hinT and ptsG are separated by 190 nucleotides
LU632_RS14755: nagZ - beta-N-acetylhexosaminidase, at 2,805,485 to 2,806,513
nagZ
LU632_RS14760: thiK - thiamine kinase, at 2,806,523 to 2,807,392
thiK
LU632_RS14765: lpoB - penicillin-binding protein activator LpoB, at 2,807,352 to 2,807,918
lpoB
LU632_RS14770: LU632_RS14770 - YcfL family protein, at 2,807,960 to 2,808,307
_RS14770
LU632_RS14775: hinT - purine nucleoside phosphoramidase, at 2,808,357 to 2,808,707
hinT
LU632_RS14780: ptsG - PTS glucose transporter subunit IIBC, at 2,808,898 to 2,810,331
ptsG
Position (kb)
2807
2808 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2806.405 kb on + strand, within nagZ at 2806.405 kb on + strand, within nagZ at 2806.420 kb on + strand at 2806.431 kb on - strand at 2806.477 kb on - strand at 2806.490 kb on + strand at 2806.491 kb on - strand at 2806.491 kb on - strand at 2806.491 kb on - strand at 2806.491 kb on - strand at 2806.491 kb on - strand at 2806.493 kb on + strand at 2806.567 kb on + strand at 2806.568 kb on - strand at 2806.605 kb on + strand at 2806.664 kb on + strand, within thiK at 2806.664 kb on + strand, within thiK at 2806.664 kb on + strand, within thiK at 2806.664 kb on + strand, within thiK at 2806.664 kb on + strand, within thiK at 2806.695 kb on + strand, within thiK at 2806.743 kb on - strand, within thiK at 2806.743 kb on - strand, within thiK at 2806.826 kb on + strand, within thiK at 2806.826 kb on + strand, within thiK at 2806.827 kb on - strand, within thiK at 2806.827 kb on - strand, within thiK at 2806.828 kb on + strand, within thiK at 2806.829 kb on - strand, within thiK at 2806.846 kb on + strand, within thiK at 2807.056 kb on + strand, within thiK at 2807.165 kb on + strand, within thiK at 2807.166 kb on - strand, within thiK at 2807.253 kb on - strand, within thiK at 2807.288 kb on + strand, within thiK at 2807.288 kb on + strand, within thiK at 2807.291 kb on - strand, within thiK at 2807.291 kb on - strand, within thiK at 2807.453 kb on + strand, within lpoB at 2807.453 kb on + strand, within lpoB at 2807.457 kb on - strand, within lpoB at 2807.457 kb on - strand, within lpoB at 2807.458 kb on + strand, within lpoB at 2807.467 kb on + strand, within lpoB at 2807.655 kb on - strand, within lpoB at 2807.655 kb on - strand, within lpoB at 2807.784 kb on + strand, within lpoB at 2807.934 kb on - strand at 2807.934 kb on - strand at 2807.961 kb on + strand at 2807.967 kb on + strand at 2807.986 kb on + strand at 2807.986 kb on + strand at 2807.987 kb on - strand at 2807.987 kb on - strand at 2808.096 kb on + strand, within LU632_RS14770 at 2808.097 kb on - strand, within LU632_RS14770 at 2808.112 kb on - strand, within LU632_RS14770 at 2808.116 kb on - strand, within LU632_RS14770 at 2808.208 kb on + strand, within LU632_RS14770 at 2808.209 kb on - strand, within LU632_RS14770 at 2808.209 kb on - strand, within LU632_RS14770 at 2808.209 kb on - strand, within LU632_RS14770 at 2808.209 kb on - strand, within LU632_RS14770 at 2808.263 kb on - strand, within LU632_RS14770 at 2808.320 kb on + strand at 2808.351 kb on + strand at 2808.449 kb on + strand, within hinT at 2808.573 kb on - strand, within hinT at 2808.653 kb on + strand, within hinT at 2808.654 kb on - strand, within hinT at 2808.678 kb on - strand at 2808.683 kb on + strand at 2808.831 kb on + strand at 2808.831 kb on + strand at 2808.832 kb on - strand at 2808.900 kb on - strand at 2808.914 kb on + strand at 2808.914 kb on + strand at 2808.914 kb on + strand at 2808.915 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Glucose remove 2,806,405 + nagZ LU632_RS14755 0.89 -1.1 2,806,405 + nagZ LU632_RS14755 0.89 +0.5 2,806,420 + +0.0 2,806,431 - +0.1 2,806,477 - +0.3 2,806,490 + +0.2 2,806,491 - -0.5 2,806,491 - -3.0 2,806,491 - +0.5 2,806,491 - +0.1 2,806,491 - +0.6 2,806,493 + -0.1 2,806,567 + +0.5 2,806,568 - -0.1 2,806,605 + +0.7 2,806,664 + thiK LU632_RS14760 0.16 -0.1 2,806,664 + thiK LU632_RS14760 0.16 -0.0 2,806,664 + thiK LU632_RS14760 0.16 -1.1 2,806,664 + thiK LU632_RS14760 0.16 -0.4 2,806,664 + thiK LU632_RS14760 0.16 +0.1 2,806,695 + thiK LU632_RS14760 0.20 +1.0 2,806,743 - thiK LU632_RS14760 0.25 -1.0 2,806,743 - thiK LU632_RS14760 0.25 -0.0 2,806,826 + thiK LU632_RS14760 0.35 +0.2 2,806,826 + thiK LU632_RS14760 0.35 +0.9 2,806,827 - thiK LU632_RS14760 0.35 +0.9 2,806,827 - thiK LU632_RS14760 0.35 +0.3 2,806,828 + thiK LU632_RS14760 0.35 -0.3 2,806,829 - thiK LU632_RS14760 0.35 -0.1 2,806,846 + thiK LU632_RS14760 0.37 +0.5 2,807,056 + thiK LU632_RS14760 0.61 -0.4 2,807,165 + thiK LU632_RS14760 0.74 +2.6 2,807,166 - thiK LU632_RS14760 0.74 -0.5 2,807,253 - thiK LU632_RS14760 0.84 -0.5 2,807,288 + thiK LU632_RS14760 0.88 -0.2 2,807,288 + thiK LU632_RS14760 0.88 -0.2 2,807,291 - thiK LU632_RS14760 0.88 -1.1 2,807,291 - thiK LU632_RS14760 0.88 +0.0 2,807,453 + lpoB LU632_RS14765 0.18 +0.0 2,807,453 + lpoB LU632_RS14765 0.18 +0.1 2,807,457 - lpoB LU632_RS14765 0.19 -0.8 2,807,457 - lpoB LU632_RS14765 0.19 -0.4 2,807,458 + lpoB LU632_RS14765 0.19 -0.0 2,807,467 + lpoB LU632_RS14765 0.20 -1.5 2,807,655 - lpoB LU632_RS14765 0.53 -0.4 2,807,655 - lpoB LU632_RS14765 0.53 -0.1 2,807,784 + lpoB LU632_RS14765 0.76 -0.7 2,807,934 - +0.9 2,807,934 - +0.2 2,807,961 + -0.1 2,807,967 + +0.4 2,807,986 + -0.3 2,807,986 + -0.2 2,807,987 - +0.2 2,807,987 - -0.4 2,808,096 + LU632_RS14770 0.39 -0.9 2,808,097 - LU632_RS14770 0.39 +0.5 2,808,112 - LU632_RS14770 0.44 +1.3 2,808,116 - LU632_RS14770 0.45 +0.2 2,808,208 + LU632_RS14770 0.71 -1.1 2,808,209 - LU632_RS14770 0.72 -0.3 2,808,209 - LU632_RS14770 0.72 -0.3 2,808,209 - LU632_RS14770 0.72 -0.2 2,808,209 - LU632_RS14770 0.72 +0.2 2,808,263 - LU632_RS14770 0.87 -0.7 2,808,320 + +0.1 2,808,351 + +2.0 2,808,449 + hinT LU632_RS14775 0.26 -0.7 2,808,573 - hinT LU632_RS14775 0.62 -0.6 2,808,653 + hinT LU632_RS14775 0.84 -0.0 2,808,654 - hinT LU632_RS14775 0.85 -1.5 2,808,678 - -0.6 2,808,683 + +0.1 2,808,831 + +0.5 2,808,831 + +0.1 2,808,832 - -0.4 2,808,900 - -0.1 2,808,914 + -1.1 2,808,914 + -1.8 2,808,914 + -0.5 2,808,915 - -2.4
Or see this region's nucleotide sequence