Experiment: HBM-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ansA and pncA are separated by 11 nucleotides pncA and LU632_RS12985 are separated by 0 nucleotides LU632_RS12985 and msrB are separated by 5 nucleotides msrB and gapA are separated by 341 nucleotides
LU632_RS12975: ansA - asparaginase, at 2,474,876 to 2,475,892
ansA
LU632_RS12980: pncA - bifunctional nicotinamidase/pyrazinamidase, at 2,475,904 to 2,476,524
pncA
LU632_RS12985: LU632_RS12985 - DUF1315 family protein, at 2,476,525 to 2,476,803
_RS12985
LU632_RS12990: msrB - peptide-methionine (R)-S-oxide reductase MsrB, at 2,476,809 to 2,477,219
msrB
LU632_RS12995: gapA - glyceraldehyde-3-phosphate dehydrogenase, at 2,477,561 to 2,478,556
gapA
Position (kb)
2476
2477 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2475.611 kb on - strand, within ansA at 2475.645 kb on + strand, within ansA at 2475.672 kb on + strand, within ansA at 2475.676 kb on + strand, within ansA at 2475.677 kb on - strand, within ansA at 2475.677 kb on - strand, within ansA at 2475.709 kb on - strand, within ansA at 2475.712 kb on + strand, within ansA at 2475.713 kb on - strand, within ansA at 2475.713 kb on - strand, within ansA at 2475.772 kb on + strand, within ansA at 2475.810 kb on + strand at 2475.813 kb on + strand at 2476.251 kb on + strand, within pncA at 2476.264 kb on + strand, within pncA at 2476.277 kb on - strand, within pncA at 2476.388 kb on + strand, within pncA at 2476.563 kb on + strand, within LU632_RS12985 at 2476.564 kb on - strand, within LU632_RS12985 at 2476.567 kb on - strand, within LU632_RS12985 at 2476.567 kb on - strand, within LU632_RS12985 at 2476.629 kb on + strand, within LU632_RS12985 at 2476.630 kb on - strand, within LU632_RS12985 at 2476.648 kb on - strand, within LU632_RS12985 at 2476.761 kb on - strand, within LU632_RS12985 at 2476.786 kb on - strand at 2476.803 kb on + strand at 2476.823 kb on + strand at 2476.823 kb on + strand at 2476.849 kb on - strand at 2476.862 kb on + strand, within msrB at 2476.863 kb on - strand, within msrB at 2476.863 kb on - strand, within msrB at 2476.865 kb on + strand, within msrB at 2476.865 kb on + strand, within msrB at 2476.865 kb on + strand, within msrB at 2476.866 kb on - strand, within msrB at 2476.866 kb on - strand, within msrB at 2477.011 kb on + strand, within msrB at 2477.041 kb on + strand, within msrB at 2477.042 kb on - strand, within msrB at 2477.042 kb on - strand, within msrB at 2477.089 kb on + strand, within msrB at 2477.090 kb on - strand, within msrB at 2477.090 kb on - strand, within msrB at 2477.090 kb on - strand, within msrB at 2477.121 kb on - strand, within msrB at 2477.121 kb on - strand, within msrB at 2477.161 kb on + strand, within msrB at 2477.162 kb on - strand, within msrB at 2477.253 kb on - strand at 2477.301 kb on + strand at 2477.375 kb on + strand at 2477.442 kb on - strand at 2477.475 kb on - strand at 2477.475 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Glucose remove 2,475,611 - ansA LU632_RS12975 0.72 +0.7 2,475,645 + ansA LU632_RS12975 0.76 -0.2 2,475,672 + ansA LU632_RS12975 0.78 -0.1 2,475,676 + ansA LU632_RS12975 0.79 -0.1 2,475,677 - ansA LU632_RS12975 0.79 +0.0 2,475,677 - ansA LU632_RS12975 0.79 -0.6 2,475,709 - ansA LU632_RS12975 0.82 +0.1 2,475,712 + ansA LU632_RS12975 0.82 +0.8 2,475,713 - ansA LU632_RS12975 0.82 -0.4 2,475,713 - ansA LU632_RS12975 0.82 -0.2 2,475,772 + ansA LU632_RS12975 0.88 +0.6 2,475,810 + -2.6 2,475,813 + +0.4 2,476,251 + pncA LU632_RS12980 0.56 +0.4 2,476,264 + pncA LU632_RS12980 0.58 -0.3 2,476,277 - pncA LU632_RS12980 0.60 -0.1 2,476,388 + pncA LU632_RS12980 0.78 -0.4 2,476,563 + LU632_RS12985 0.14 +1.0 2,476,564 - LU632_RS12985 0.14 +0.0 2,476,567 - LU632_RS12985 0.15 -0.0 2,476,567 - LU632_RS12985 0.15 +0.0 2,476,629 + LU632_RS12985 0.37 -0.4 2,476,630 - LU632_RS12985 0.38 -0.6 2,476,648 - LU632_RS12985 0.44 +0.2 2,476,761 - LU632_RS12985 0.85 -0.2 2,476,786 - -0.2 2,476,803 + +2.0 2,476,823 + -0.6 2,476,823 + -0.2 2,476,849 - -0.2 2,476,862 + msrB LU632_RS12990 0.13 -0.6 2,476,863 - msrB LU632_RS12990 0.13 -1.3 2,476,863 - msrB LU632_RS12990 0.13 -0.5 2,476,865 + msrB LU632_RS12990 0.14 +0.1 2,476,865 + msrB LU632_RS12990 0.14 -0.8 2,476,865 + msrB LU632_RS12990 0.14 -0.4 2,476,866 - msrB LU632_RS12990 0.14 -0.2 2,476,866 - msrB LU632_RS12990 0.14 +0.1 2,477,011 + msrB LU632_RS12990 0.49 +0.2 2,477,041 + msrB LU632_RS12990 0.56 -0.2 2,477,042 - msrB LU632_RS12990 0.57 -0.3 2,477,042 - msrB LU632_RS12990 0.57 +0.4 2,477,089 + msrB LU632_RS12990 0.68 +0.0 2,477,090 - msrB LU632_RS12990 0.68 -0.2 2,477,090 - msrB LU632_RS12990 0.68 +0.7 2,477,090 - msrB LU632_RS12990 0.68 +0.4 2,477,121 - msrB LU632_RS12990 0.76 -0.4 2,477,121 - msrB LU632_RS12990 0.76 -0.3 2,477,161 + msrB LU632_RS12990 0.86 +1.1 2,477,162 - msrB LU632_RS12990 0.86 -0.6 2,477,253 - -0.3 2,477,301 + -0.5 2,477,375 + -0.6 2,477,442 - +0.3 2,477,475 - -1.3 2,477,475 - +0.4
Or see this region's nucleotide sequence