Experiment: HBM-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyrD and LU632_RS09960 are separated by 161 nucleotides LU632_RS09960 and rlmKL are separated by 662 nucleotides
LU632_RS09955: pyrD - quinone-dependent dihydroorotate dehydrogenase, at 1,901,346 to 1,902,356
pyrD
LU632_RS09960: LU632_RS09960 - cell division protein ZapC, at 1,902,518 to 1,903,063
_RS09960
LU632_RS09965: rlmKL - bifunctional 23S rRNA (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA (guanine(2445)-N(2))-methyltransferase RlmL, at 1,903,726 to 1,905,843
rlmKL
Position (kb)
1902
1903
1904 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1901.555 kb on + strand, within pyrD at 1901.555 kb on + strand, within pyrD at 1901.556 kb on - strand, within pyrD at 1901.631 kb on - strand, within pyrD at 1901.634 kb on - strand, within pyrD at 1901.749 kb on + strand, within pyrD at 1901.750 kb on - strand, within pyrD at 1901.753 kb on - strand, within pyrD at 1901.780 kb on + strand, within pyrD at 1901.780 kb on + strand, within pyrD at 1901.781 kb on - strand, within pyrD at 1901.896 kb on + strand, within pyrD at 1901.896 kb on + strand, within pyrD at 1901.896 kb on + strand, within pyrD at 1901.972 kb on + strand, within pyrD at 1901.973 kb on - strand, within pyrD at 1901.976 kb on + strand, within pyrD at 1901.977 kb on - strand, within pyrD at 1902.056 kb on + strand, within pyrD at 1902.059 kb on + strand, within pyrD at 1902.059 kb on + strand, within pyrD at 1902.084 kb on - strand, within pyrD at 1902.086 kb on + strand, within pyrD at 1902.086 kb on + strand, within pyrD at 1902.086 kb on + strand, within pyrD at 1902.087 kb on - strand, within pyrD at 1902.369 kb on + strand at 1902.412 kb on + strand at 1902.435 kb on + strand at 1902.435 kb on + strand at 1902.436 kb on - strand at 1902.436 kb on - strand at 1902.574 kb on + strand, within LU632_RS09960 at 1902.583 kb on - strand, within LU632_RS09960 at 1902.790 kb on + strand, within LU632_RS09960 at 1902.790 kb on + strand, within LU632_RS09960 at 1902.791 kb on - strand, within LU632_RS09960 at 1902.791 kb on - strand, within LU632_RS09960 at 1902.791 kb on - strand, within LU632_RS09960 at 1902.812 kb on - strand, within LU632_RS09960 at 1902.930 kb on + strand, within LU632_RS09960 at 1902.930 kb on + strand, within LU632_RS09960 at 1902.973 kb on + strand, within LU632_RS09960 at 1902.973 kb on + strand, within LU632_RS09960 at 1903.137 kb on + strand at 1903.138 kb on + strand at 1903.138 kb on - strand at 1903.139 kb on + strand at 1903.139 kb on + strand at 1903.142 kb on + strand at 1903.143 kb on - strand at 1903.156 kb on + strand at 1903.224 kb on + strand at 1903.225 kb on - strand at 1903.255 kb on - strand at 1903.362 kb on + strand at 1903.362 kb on + strand at 1903.453 kb on - strand at 1903.574 kb on - strand at 1903.574 kb on - strand at 1903.578 kb on - strand at 1903.632 kb on + strand at 1903.682 kb on + strand at 1903.686 kb on + strand at 1903.688 kb on + strand at 1903.829 kb on + strand at 1903.830 kb on - strand at 1903.834 kb on + strand at 1903.834 kb on + strand at 1903.835 kb on - strand at 1903.835 kb on - strand at 1903.835 kb on - strand at 1903.835 kb on - strand at 1903.836 kb on + strand at 1903.837 kb on - strand at 1903.893 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Glucose remove 1,901,555 + pyrD LU632_RS09955 0.21 -2.7 1,901,555 + pyrD LU632_RS09955 0.21 -2.7 1,901,556 - pyrD LU632_RS09955 0.21 -2.4 1,901,631 - pyrD LU632_RS09955 0.28 -4.3 1,901,634 - pyrD LU632_RS09955 0.28 -4.4 1,901,749 + pyrD LU632_RS09955 0.40 -2.7 1,901,750 - pyrD LU632_RS09955 0.40 -2.4 1,901,753 - pyrD LU632_RS09955 0.40 -4.5 1,901,780 + pyrD LU632_RS09955 0.43 -2.1 1,901,780 + pyrD LU632_RS09955 0.43 -3.7 1,901,781 - pyrD LU632_RS09955 0.43 -4.3 1,901,896 + pyrD LU632_RS09955 0.54 -4.6 1,901,896 + pyrD LU632_RS09955 0.54 -3.6 1,901,896 + pyrD LU632_RS09955 0.54 -5.6 1,901,972 + pyrD LU632_RS09955 0.62 -1.4 1,901,973 - pyrD LU632_RS09955 0.62 -5.1 1,901,976 + pyrD LU632_RS09955 0.62 -3.2 1,901,977 - pyrD LU632_RS09955 0.62 -2.4 1,902,056 + pyrD LU632_RS09955 0.70 -4.1 1,902,059 + pyrD LU632_RS09955 0.71 -2.6 1,902,059 + pyrD LU632_RS09955 0.71 -4.9 1,902,084 - pyrD LU632_RS09955 0.73 -5.7 1,902,086 + pyrD LU632_RS09955 0.73 -4.8 1,902,086 + pyrD LU632_RS09955 0.73 -1.9 1,902,086 + pyrD LU632_RS09955 0.73 -4.1 1,902,087 - pyrD LU632_RS09955 0.73 -3.9 1,902,369 + +0.0 1,902,412 + +0.3 1,902,435 + -0.0 1,902,435 + -0.2 1,902,436 - +0.2 1,902,436 - -0.1 1,902,574 + LU632_RS09960 0.10 -1.4 1,902,583 - LU632_RS09960 0.12 -0.6 1,902,790 + LU632_RS09960 0.50 -0.0 1,902,790 + LU632_RS09960 0.50 +0.1 1,902,791 - LU632_RS09960 0.50 +0.4 1,902,791 - LU632_RS09960 0.50 -1.2 1,902,791 - LU632_RS09960 0.50 +0.2 1,902,812 - LU632_RS09960 0.54 +0.7 1,902,930 + LU632_RS09960 0.75 -0.4 1,902,930 + LU632_RS09960 0.75 +0.2 1,902,973 + LU632_RS09960 0.83 -0.1 1,902,973 + LU632_RS09960 0.83 -1.3 1,903,137 + +0.2 1,903,138 + +0.0 1,903,138 - -0.9 1,903,139 + +0.3 1,903,139 + -0.4 1,903,142 + -0.4 1,903,143 - -0.7 1,903,156 + -0.1 1,903,224 + +0.1 1,903,225 - -0.3 1,903,255 - -0.2 1,903,362 + +0.4 1,903,362 + +0.4 1,903,453 - -0.6 1,903,574 - +0.4 1,903,574 - -0.3 1,903,578 - -1.2 1,903,632 + +0.2 1,903,682 + +0.1 1,903,686 + +0.7 1,903,688 + +0.1 1,903,829 + -0.7 1,903,830 - +0.3 1,903,834 + -0.1 1,903,834 + +0.4 1,903,835 - -1.1 1,903,835 - +0.9 1,903,835 - -1.6 1,903,835 - +0.2 1,903,836 + +1.1 1,903,837 - +0.9 1,903,893 + -1.2
Or see this region's nucleotide sequence