Experiment: HBM-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisA and hisH are separated by 5 nucleotides hisH and hisB are separated by 0 nucleotides hisB and hisC overlap by 4 nucleotides
LU632_RS08925: hisA - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 1,684,845 to 1,685,582
hisA
LU632_RS08930: hisH - imidazole glycerol phosphate synthase subunit HisH, at 1,685,588 to 1,686,178
hisH
LU632_RS08935: hisB - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 1,686,179 to 1,687,246
hisB
LU632_RS08940: hisC - histidinol-phosphate transaminase, at 1,687,243 to 1,688,334
hisC
Position (kb)
1686
1687
1688 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1685.234 kb on + strand, within hisA at 1685.234 kb on + strand, within hisA at 1685.471 kb on - strand, within hisA at 1685.478 kb on + strand, within hisA at 1685.503 kb on + strand, within hisA at 1685.576 kb on + strand at 1685.589 kb on + strand at 1685.590 kb on - strand at 1685.703 kb on + strand, within hisH at 1685.703 kb on + strand, within hisH at 1685.703 kb on + strand, within hisH at 1685.704 kb on - strand, within hisH at 1685.704 kb on - strand, within hisH at 1685.707 kb on + strand, within hisH at 1685.708 kb on - strand, within hisH at 1685.708 kb on - strand, within hisH at 1685.708 kb on - strand, within hisH at 1685.708 kb on - strand, within hisH at 1685.708 kb on - strand, within hisH at 1685.745 kb on + strand, within hisH at 1685.745 kb on + strand, within hisH at 1685.746 kb on - strand, within hisH at 1685.746 kb on - strand, within hisH at 1685.746 kb on - strand, within hisH at 1685.760 kb on + strand, within hisH at 1685.766 kb on + strand, within hisH at 1685.766 kb on + strand, within hisH at 1685.767 kb on - strand, within hisH at 1685.767 kb on - strand, within hisH at 1685.767 kb on - strand, within hisH at 1686.181 kb on - strand at 1686.181 kb on - strand at 1686.368 kb on + strand, within hisB at 1686.403 kb on + strand, within hisB at 1686.403 kb on + strand, within hisB at 1686.403 kb on + strand, within hisB at 1686.404 kb on - strand, within hisB at 1686.405 kb on + strand, within hisB at 1686.405 kb on + strand, within hisB at 1686.573 kb on + strand, within hisB at 1686.574 kb on - strand, within hisB at 1686.574 kb on - strand, within hisB at 1686.595 kb on + strand, within hisB at 1686.638 kb on - strand, within hisB at 1686.638 kb on - strand, within hisB at 1686.638 kb on - strand, within hisB at 1686.638 kb on - strand, within hisB at 1686.638 kb on - strand, within hisB at 1686.638 kb on - strand, within hisB at 1686.725 kb on - strand, within hisB at 1686.769 kb on + strand, within hisB at 1686.805 kb on - strand, within hisB at 1686.827 kb on - strand, within hisB at 1686.961 kb on + strand, within hisB at 1686.961 kb on + strand, within hisB at 1686.961 kb on + strand, within hisB at 1686.962 kb on - strand, within hisB at 1686.995 kb on - strand, within hisB at 1687.162 kb on + strand at 1687.204 kb on + strand at 1687.380 kb on + strand, within hisC at 1687.380 kb on + strand, within hisC at 1687.442 kb on + strand, within hisC at 1687.442 kb on + strand, within hisC at 1687.442 kb on + strand, within hisC at 1687.442 kb on + strand, within hisC at 1687.442 kb on + strand, within hisC at 1687.442 kb on + strand, within hisC at 1687.443 kb on - strand, within hisC at 1687.443 kb on - strand, within hisC at 1687.443 kb on - strand, within hisC at 1687.443 kb on - strand, within hisC at 1687.521 kb on + strand, within hisC at 1687.527 kb on + strand, within hisC at 1687.676 kb on - strand, within hisC at 1687.711 kb on + strand, within hisC at 1687.716 kb on + strand, within hisC at 1687.774 kb on + strand, within hisC at 1687.774 kb on + strand, within hisC at 1687.774 kb on + strand, within hisC at 1687.777 kb on - strand, within hisC at 1687.777 kb on - strand, within hisC at 1687.778 kb on + strand, within hisC at 1687.778 kb on + strand, within hisC at 1687.778 kb on + strand, within hisC at 1687.778 kb on + strand, within hisC at 1687.778 kb on + strand, within hisC at 1687.778 kb on + strand, within hisC at 1687.779 kb on - strand, within hisC at 1687.886 kb on + strand, within hisC at 1687.958 kb on - strand, within hisC at 1687.958 kb on - strand, within hisC at 1688.000 kb on + strand, within hisC at 1688.000 kb on + strand, within hisC at 1688.001 kb on - strand, within hisC at 1688.001 kb on - strand, within hisC at 1688.005 kb on - strand, within hisC at 1688.129 kb on + strand, within hisC at 1688.223 kb on - strand, within hisC at 1688.242 kb on + strand at 1688.242 kb on + strand at 1688.242 kb on + strand at 1688.242 kb on + strand at 1688.242 kb on + strand at 1688.243 kb on - strand at 1688.243 kb on - strand at 1688.243 kb on - strand at 1688.243 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Glucose remove 1,685,234 + hisA LU632_RS08925 0.53 -0.8 1,685,234 + hisA LU632_RS08925 0.53 -1.1 1,685,471 - hisA LU632_RS08925 0.85 -3.6 1,685,478 + hisA LU632_RS08925 0.86 -1.8 1,685,503 + hisA LU632_RS08925 0.89 -3.5 1,685,576 + -4.7 1,685,589 + -2.1 1,685,590 - -1.1 1,685,703 + hisH LU632_RS08930 0.19 -2.1 1,685,703 + hisH LU632_RS08930 0.19 -3.2 1,685,703 + hisH LU632_RS08930 0.19 -1.5 1,685,704 - hisH LU632_RS08930 0.20 -4.8 1,685,704 - hisH LU632_RS08930 0.20 -5.0 1,685,707 + hisH LU632_RS08930 0.20 -1.8 1,685,708 - hisH LU632_RS08930 0.20 -4.2 1,685,708 - hisH LU632_RS08930 0.20 -2.9 1,685,708 - hisH LU632_RS08930 0.20 -2.9 1,685,708 - hisH LU632_RS08930 0.20 -2.8 1,685,708 - hisH LU632_RS08930 0.20 -5.3 1,685,745 + hisH LU632_RS08930 0.27 -3.4 1,685,745 + hisH LU632_RS08930 0.27 -4.3 1,685,746 - hisH LU632_RS08930 0.27 -3.7 1,685,746 - hisH LU632_RS08930 0.27 -3.2 1,685,746 - hisH LU632_RS08930 0.27 -1.1 1,685,760 + hisH LU632_RS08930 0.29 -4.3 1,685,766 + hisH LU632_RS08930 0.30 +0.5 1,685,766 + hisH LU632_RS08930 0.30 -2.8 1,685,767 - hisH LU632_RS08930 0.30 -2.7 1,685,767 - hisH LU632_RS08930 0.30 -4.7 1,685,767 - hisH LU632_RS08930 0.30 -4.0 1,686,181 - -2.5 1,686,181 - -1.6 1,686,368 + hisB LU632_RS08935 0.18 -4.1 1,686,403 + hisB LU632_RS08935 0.21 -0.9 1,686,403 + hisB LU632_RS08935 0.21 -2.9 1,686,403 + hisB LU632_RS08935 0.21 -4.5 1,686,404 - hisB LU632_RS08935 0.21 -0.1 1,686,405 + hisB LU632_RS08935 0.21 -3.1 1,686,405 + hisB LU632_RS08935 0.21 -4.3 1,686,573 + hisB LU632_RS08935 0.37 -3.3 1,686,574 - hisB LU632_RS08935 0.37 -2.1 1,686,574 - hisB LU632_RS08935 0.37 -0.5 1,686,595 + hisB LU632_RS08935 0.39 -4.7 1,686,638 - hisB LU632_RS08935 0.43 -3.1 1,686,638 - hisB LU632_RS08935 0.43 -2.3 1,686,638 - hisB LU632_RS08935 0.43 -2.2 1,686,638 - hisB LU632_RS08935 0.43 -3.3 1,686,638 - hisB LU632_RS08935 0.43 -2.3 1,686,638 - hisB LU632_RS08935 0.43 -1.5 1,686,725 - hisB LU632_RS08935 0.51 -1.8 1,686,769 + hisB LU632_RS08935 0.55 -1.7 1,686,805 - hisB LU632_RS08935 0.59 -3.1 1,686,827 - hisB LU632_RS08935 0.61 -1.1 1,686,961 + hisB LU632_RS08935 0.73 -1.4 1,686,961 + hisB LU632_RS08935 0.73 -2.1 1,686,961 + hisB LU632_RS08935 0.73 -2.9 1,686,962 - hisB LU632_RS08935 0.73 -3.1 1,686,995 - hisB LU632_RS08935 0.76 -3.2 1,687,162 + -2.5 1,687,204 + -2.8 1,687,380 + hisC LU632_RS08940 0.13 -2.9 1,687,380 + hisC LU632_RS08940 0.13 -0.8 1,687,442 + hisC LU632_RS08940 0.18 -3.2 1,687,442 + hisC LU632_RS08940 0.18 -2.5 1,687,442 + hisC LU632_RS08940 0.18 -1.9 1,687,442 + hisC LU632_RS08940 0.18 -2.8 1,687,442 + hisC LU632_RS08940 0.18 -1.5 1,687,442 + hisC LU632_RS08940 0.18 -1.7 1,687,443 - hisC LU632_RS08940 0.18 -1.8 1,687,443 - hisC LU632_RS08940 0.18 -2.5 1,687,443 - hisC LU632_RS08940 0.18 -2.8 1,687,443 - hisC LU632_RS08940 0.18 -1.1 1,687,521 + hisC LU632_RS08940 0.25 -1.6 1,687,527 + hisC LU632_RS08940 0.26 -2.8 1,687,676 - hisC LU632_RS08940 0.40 -2.7 1,687,711 + hisC LU632_RS08940 0.43 -1.5 1,687,716 + hisC LU632_RS08940 0.43 -2.7 1,687,774 + hisC LU632_RS08940 0.49 -3.2 1,687,774 + hisC LU632_RS08940 0.49 -1.5 1,687,774 + hisC LU632_RS08940 0.49 -2.1 1,687,777 - hisC LU632_RS08940 0.49 -2.5 1,687,777 - hisC LU632_RS08940 0.49 -2.6 1,687,778 + hisC LU632_RS08940 0.49 -3.4 1,687,778 + hisC LU632_RS08940 0.49 -1.4 1,687,778 + hisC LU632_RS08940 0.49 -1.4 1,687,778 + hisC LU632_RS08940 0.49 -2.5 1,687,778 + hisC LU632_RS08940 0.49 -2.1 1,687,778 + hisC LU632_RS08940 0.49 -1.3 1,687,779 - hisC LU632_RS08940 0.49 +0.5 1,687,886 + hisC LU632_RS08940 0.59 -0.5 1,687,958 - hisC LU632_RS08940 0.65 -3.8 1,687,958 - hisC LU632_RS08940 0.65 +0.1 1,688,000 + hisC LU632_RS08940 0.69 -1.2 1,688,000 + hisC LU632_RS08940 0.69 -1.8 1,688,001 - hisC LU632_RS08940 0.69 -2.8 1,688,001 - hisC LU632_RS08940 0.69 -2.0 1,688,005 - hisC LU632_RS08940 0.70 -2.8 1,688,129 + hisC LU632_RS08940 0.81 -2.6 1,688,223 - hisC LU632_RS08940 0.90 +1.5 1,688,242 + -2.6 1,688,242 + -4.7 1,688,242 + -3.5 1,688,242 + -2.7 1,688,242 + -1.1 1,688,243 - -3.1 1,688,243 - -3.1 1,688,243 - -2.3 1,688,243 - -3.3
Or see this region's nucleotide sequence