Experiment: HBM-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS05430 and LU632_RS05435 are separated by 65 nucleotides LU632_RS05435 and LU632_RS05440 are separated by 60 nucleotides LU632_RS05440 and hemW are separated by 146 nucleotides
LU632_RS05430: LU632_RS05430 - YggL family protein, at 1,049,210 to 1,049,536
_RS05430
LU632_RS05435: LU632_RS05435 - DUF2884 domain-containing protein, at 1,049,602 to 1,050,321
_RS05435
LU632_RS05440: LU632_RS05440 - helix-turn-helix domain-containing protein, at 1,050,382 to 1,051,423
_RS05440
LU632_RS05445: hemW - radical SAM family heme chaperone HemW, at 1,051,570 to 1,052,730
hemW
Position (kb)
1050
1051
1052 Strain fitness (log2 ratio)
-2
-1
0
1 at 1049.535 kb on - strand at 1049.607 kb on + strand at 1049.607 kb on + strand at 1049.630 kb on + strand at 1049.662 kb on + strand at 1049.714 kb on + strand, within LU632_RS05435 at 1049.714 kb on + strand, within LU632_RS05435 at 1049.715 kb on - strand, within LU632_RS05435 at 1049.998 kb on - strand, within LU632_RS05435 at 1050.044 kb on + strand, within LU632_RS05435 at 1050.045 kb on - strand, within LU632_RS05435 at 1050.084 kb on + strand, within LU632_RS05435 at 1050.085 kb on - strand, within LU632_RS05435 at 1050.291 kb on + strand at 1050.291 kb on + strand at 1050.292 kb on - strand at 1050.292 kb on - strand at 1050.330 kb on - strand at 1050.330 kb on - strand at 1050.383 kb on + strand at 1050.413 kb on - strand at 1051.522 kb on + strand at 1051.704 kb on + strand, within hemW at 1051.720 kb on - strand, within hemW at 1051.860 kb on + strand, within hemW at 1051.971 kb on - strand, within hemW at 1051.971 kb on - strand, within hemW at 1052.010 kb on - strand, within hemW at 1052.024 kb on + strand, within hemW at 1052.025 kb on - strand, within hemW at 1052.246 kb on - strand, within hemW at 1052.246 kb on - strand, within hemW at 1052.250 kb on + strand, within hemW at 1052.251 kb on - strand, within hemW at 1052.251 kb on - strand, within hemW at 1052.370 kb on + strand, within hemW at 1052.370 kb on + strand, within hemW at 1052.370 kb on + strand, within hemW
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Glucose remove 1,049,535 - -1.7 1,049,607 + -0.1 1,049,607 + +0.3 1,049,630 + -0.2 1,049,662 + +0.6 1,049,714 + LU632_RS05435 0.16 +0.2 1,049,714 + LU632_RS05435 0.16 -0.8 1,049,715 - LU632_RS05435 0.16 -0.3 1,049,998 - LU632_RS05435 0.55 -0.9 1,050,044 + LU632_RS05435 0.61 +0.4 1,050,045 - LU632_RS05435 0.62 -0.5 1,050,084 + LU632_RS05435 0.67 +0.5 1,050,085 - LU632_RS05435 0.67 +0.7 1,050,291 + -1.1 1,050,291 + -0.0 1,050,292 - +0.5 1,050,292 - -0.7 1,050,330 - -0.1 1,050,330 - +0.3 1,050,383 + +0.0 1,050,413 - -0.9 1,051,522 + +0.2 1,051,704 + hemW LU632_RS05445 0.12 -1.0 1,051,720 - hemW LU632_RS05445 0.13 +1.1 1,051,860 + hemW LU632_RS05445 0.25 -0.3 1,051,971 - hemW LU632_RS05445 0.35 +0.3 1,051,971 - hemW LU632_RS05445 0.35 -1.0 1,052,010 - hemW LU632_RS05445 0.38 -1.1 1,052,024 + hemW LU632_RS05445 0.39 +0.3 1,052,025 - hemW LU632_RS05445 0.39 +0.4 1,052,246 - hemW LU632_RS05445 0.58 -0.3 1,052,246 - hemW LU632_RS05445 0.58 -0.1 1,052,250 + hemW LU632_RS05445 0.59 +1.2 1,052,251 - hemW LU632_RS05445 0.59 -2.7 1,052,251 - hemW LU632_RS05445 0.59 -2.3 1,052,370 + hemW LU632_RS05445 0.69 +0.2 1,052,370 + hemW LU632_RS05445 0.69 -1.1 1,052,370 + hemW LU632_RS05445 0.69 +0.1
Or see this region's nucleotide sequence