Experiment: HBM-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hofC and gspE overlap by 23 nucleotides gspE and ppdD overlap by 1 nucleotides ppdD and nadC are separated by 195 nucleotides
LU632_RS03025: hofC - protein transport protein HofC, at 617,881 to 619,080
hofC
LU632_RS03030: gspE - type II secretion system protein GspE, at 619,058 to 620,461
gspE
LU632_RS03035: ppdD - prepilin peptidase-dependent pilin, at 620,461 to 620,913
ppdD
LU632_RS03040: nadC - carboxylating nicotinate-nucleotide diphosphorylase, at 621,109 to 621,996
nadC
Position (kb) 619
620
621 Strain fitness (log2 ratio) -2
-1
0
1
2 at 618.092 kb on + strand, within hofC at 618.132 kb on + strand, within hofC at 618.132 kb on + strand, within hofC at 618.133 kb on - strand, within hofC at 618.135 kb on - strand, within hofC at 618.135 kb on - strand, within hofC at 618.135 kb on - strand, within hofC at 618.136 kb on + strand, within hofC at 618.136 kb on + strand, within hofC at 618.137 kb on - strand, within hofC at 618.156 kb on + strand, within hofC at 618.157 kb on - strand, within hofC at 618.223 kb on + strand, within hofC at 618.228 kb on + strand, within hofC at 618.274 kb on - strand, within hofC at 618.274 kb on - strand, within hofC at 618.274 kb on - strand, within hofC at 618.277 kb on + strand, within hofC at 618.278 kb on - strand, within hofC at 618.278 kb on - strand, within hofC at 618.348 kb on + strand, within hofC at 618.395 kb on + strand, within hofC at 618.406 kb on + strand, within hofC at 618.406 kb on + strand, within hofC at 618.407 kb on - strand, within hofC at 618.460 kb on + strand, within hofC at 618.460 kb on + strand, within hofC at 618.503 kb on + strand, within hofC at 618.503 kb on + strand, within hofC at 618.503 kb on + strand, within hofC at 618.505 kb on + strand, within hofC at 618.506 kb on - strand, within hofC at 618.527 kb on - strand, within hofC at 618.613 kb on + strand, within hofC at 618.666 kb on + strand, within hofC at 618.769 kb on - strand, within hofC at 618.864 kb on + strand, within hofC at 618.869 kb on - strand, within hofC at 618.920 kb on - strand, within hofC at 618.974 kb on - strand at 619.103 kb on + strand at 619.120 kb on + strand at 619.158 kb on - strand at 619.188 kb on - strand at 619.193 kb on + strand at 619.194 kb on - strand at 619.194 kb on - strand at 619.282 kb on - strand, within gspE at 619.504 kb on + strand, within gspE at 619.505 kb on - strand, within gspE at 619.508 kb on + strand, within gspE at 619.513 kb on + strand, within gspE at 619.513 kb on + strand, within gspE at 619.591 kb on + strand, within gspE at 619.591 kb on + strand, within gspE at 619.592 kb on - strand, within gspE at 619.595 kb on - strand, within gspE at 619.661 kb on + strand, within gspE at 619.662 kb on - strand, within gspE at 619.662 kb on - strand, within gspE at 619.714 kb on + strand, within gspE at 619.743 kb on + strand, within gspE at 619.744 kb on - strand, within gspE at 619.744 kb on - strand, within gspE at 619.744 kb on - strand, within gspE at 619.758 kb on + strand, within gspE at 619.903 kb on + strand, within gspE at 619.903 kb on + strand, within gspE at 620.008 kb on + strand, within gspE at 620.009 kb on - strand, within gspE at 620.102 kb on + strand, within gspE at 620.103 kb on - strand, within gspE at 620.105 kb on - strand, within gspE at 620.105 kb on - strand, within gspE at 620.105 kb on - strand, within gspE at 620.134 kb on + strand, within gspE at 620.159 kb on - strand, within gspE at 620.159 kb on - strand, within gspE at 620.189 kb on + strand, within gspE at 620.218 kb on + strand, within gspE at 620.295 kb on + strand, within gspE at 620.305 kb on + strand, within gspE at 620.314 kb on + strand, within gspE at 620.315 kb on - strand, within gspE at 620.324 kb on - strand at 620.342 kb on + strand at 620.342 kb on + strand at 620.343 kb on - strand at 620.392 kb on + strand at 620.428 kb on + strand at 620.428 kb on + strand at 620.429 kb on - strand at 620.559 kb on + strand, within ppdD at 620.559 kb on + strand, within ppdD at 620.560 kb on - strand, within ppdD at 620.672 kb on + strand, within ppdD at 620.673 kb on - strand, within ppdD at 620.756 kb on - strand, within ppdD at 620.767 kb on - strand, within ppdD at 620.816 kb on + strand, within ppdD at 620.817 kb on - strand, within ppdD at 620.892 kb on + strand at 620.893 kb on - strand at 620.918 kb on + strand at 620.919 kb on - strand at 621.032 kb on - strand at 621.032 kb on - strand at 621.032 kb on - strand at 621.032 kb on - strand at 621.127 kb on + strand at 621.144 kb on + strand at 621.194 kb on + strand at 621.342 kb on + strand, within nadC at 621.343 kb on - strand, within nadC at 621.455 kb on + strand, within nadC
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Glucose remove 618,092 + hofC LU632_RS03025 0.18 +1.1 618,132 + hofC LU632_RS03025 0.21 -1.2 618,132 + hofC LU632_RS03025 0.21 -1.8 618,133 - hofC LU632_RS03025 0.21 +0.2 618,135 - hofC LU632_RS03025 0.21 +0.2 618,135 - hofC LU632_RS03025 0.21 -0.3 618,135 - hofC LU632_RS03025 0.21 -0.9 618,136 + hofC LU632_RS03025 0.21 +0.5 618,136 + hofC LU632_RS03025 0.21 -1.8 618,137 - hofC LU632_RS03025 0.21 +0.3 618,156 + hofC LU632_RS03025 0.23 -1.4 618,157 - hofC LU632_RS03025 0.23 -0.3 618,223 + hofC LU632_RS03025 0.28 -1.0 618,228 + hofC LU632_RS03025 0.29 -0.3 618,274 - hofC LU632_RS03025 0.33 -0.1 618,274 - hofC LU632_RS03025 0.33 -0.5 618,274 - hofC LU632_RS03025 0.33 -0.2 618,277 + hofC LU632_RS03025 0.33 +0.7 618,278 - hofC LU632_RS03025 0.33 +0.1 618,278 - hofC LU632_RS03025 0.33 -1.2 618,348 + hofC LU632_RS03025 0.39 -0.3 618,395 + hofC LU632_RS03025 0.43 -1.5 618,406 + hofC LU632_RS03025 0.44 -0.2 618,406 + hofC LU632_RS03025 0.44 -0.8 618,407 - hofC LU632_RS03025 0.44 +0.5 618,460 + hofC LU632_RS03025 0.48 +1.1 618,460 + hofC LU632_RS03025 0.48 +0.5 618,503 + hofC LU632_RS03025 0.52 -0.3 618,503 + hofC LU632_RS03025 0.52 -2.1 618,503 + hofC LU632_RS03025 0.52 -0.2 618,505 + hofC LU632_RS03025 0.52 -0.2 618,506 - hofC LU632_RS03025 0.52 +0.8 618,527 - hofC LU632_RS03025 0.54 +0.1 618,613 + hofC LU632_RS03025 0.61 +2.3 618,666 + hofC LU632_RS03025 0.65 -1.5 618,769 - hofC LU632_RS03025 0.74 +0.3 618,864 + hofC LU632_RS03025 0.82 +0.8 618,869 - hofC LU632_RS03025 0.82 +0.0 618,920 - hofC LU632_RS03025 0.87 +0.6 618,974 - +0.1 619,103 + -0.1 619,120 + -0.3 619,158 - +0.6 619,188 - +2.3 619,193 + -0.1 619,194 - +1.0 619,194 - +0.3 619,282 - gspE LU632_RS03030 0.16 +1.8 619,504 + gspE LU632_RS03030 0.32 -1.0 619,505 - gspE LU632_RS03030 0.32 -0.2 619,508 + gspE LU632_RS03030 0.32 -0.6 619,513 + gspE LU632_RS03030 0.32 +0.1 619,513 + gspE LU632_RS03030 0.32 +0.1 619,591 + gspE LU632_RS03030 0.38 -0.1 619,591 + gspE LU632_RS03030 0.38 -1.0 619,592 - gspE LU632_RS03030 0.38 -0.1 619,595 - gspE LU632_RS03030 0.38 -0.1 619,661 + gspE LU632_RS03030 0.43 -0.4 619,662 - gspE LU632_RS03030 0.43 -0.3 619,662 - gspE LU632_RS03030 0.43 -0.5 619,714 + gspE LU632_RS03030 0.47 -1.2 619,743 + gspE LU632_RS03030 0.49 +0.0 619,744 - gspE LU632_RS03030 0.49 -0.1 619,744 - gspE LU632_RS03030 0.49 +0.5 619,744 - gspE LU632_RS03030 0.49 +1.8 619,758 + gspE LU632_RS03030 0.50 +0.5 619,903 + gspE LU632_RS03030 0.60 -0.4 619,903 + gspE LU632_RS03030 0.60 +0.8 620,008 + gspE LU632_RS03030 0.68 +0.6 620,009 - gspE LU632_RS03030 0.68 -0.3 620,102 + gspE LU632_RS03030 0.74 +1.5 620,103 - gspE LU632_RS03030 0.74 +0.3 620,105 - gspE LU632_RS03030 0.75 +0.8 620,105 - gspE LU632_RS03030 0.75 -0.0 620,105 - gspE LU632_RS03030 0.75 -0.8 620,134 + gspE LU632_RS03030 0.77 +0.2 620,159 - gspE LU632_RS03030 0.78 +0.8 620,159 - gspE LU632_RS03030 0.78 +0.3 620,189 + gspE LU632_RS03030 0.81 -0.2 620,218 + gspE LU632_RS03030 0.83 +0.5 620,295 + gspE LU632_RS03030 0.88 +0.1 620,305 + gspE LU632_RS03030 0.89 -0.2 620,314 + gspE LU632_RS03030 0.89 +0.8 620,315 - gspE LU632_RS03030 0.90 +0.3 620,324 - -0.5 620,342 + -0.6 620,342 + -0.6 620,343 - +0.0 620,392 + -1.2 620,428 + -0.4 620,428 + -0.1 620,429 - -0.3 620,559 + ppdD LU632_RS03035 0.22 -0.1 620,559 + ppdD LU632_RS03035 0.22 -0.5 620,560 - ppdD LU632_RS03035 0.22 -0.4 620,672 + ppdD LU632_RS03035 0.47 -0.3 620,673 - ppdD LU632_RS03035 0.47 +0.4 620,756 - ppdD LU632_RS03035 0.65 -0.8 620,767 - ppdD LU632_RS03035 0.68 +0.1 620,816 + ppdD LU632_RS03035 0.78 +0.8 620,817 - ppdD LU632_RS03035 0.79 -1.2 620,892 + +0.9 620,893 - -0.6 620,918 + +0.3 620,919 - +0.8 621,032 - +0.1 621,032 - +0.6 621,032 - +0.1 621,032 - -0.9 621,127 + -0.7 621,144 + +0.5 621,194 + +0.3 621,342 + nadC LU632_RS03040 0.26 +0.6 621,343 - nadC LU632_RS03040 0.26 +0.3 621,455 + nadC LU632_RS03040 0.39 +0.4
Or see this region's nucleotide sequence