Experiment: HBM-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ileS and lspA are separated by 0 nucleotides lspA and fkpB are separated by 9 nucleotides fkpB and ispH overlap by 20 nucleotides ispH and LU632_RS02700 are separated by 80 nucleotides LU632_RS02700 and LU632_RS02705 are separated by 60 nucleotides
LU632_RS02680: ileS - isoleucine--tRNA ligase, at 537,809 to 540,625
ileS
LU632_RS02685: lspA - signal peptidase II, at 540,626 to 541,120
lspA
LU632_RS02690: fkpB - FKBP-type peptidyl-prolyl cis-trans isomerase, at 541,130 to 541,600
fkpB
LU632_RS02695: ispH - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 541,581 to 542,534
ispH
LU632_RS02700: LU632_RS02700 - IS5 family transposase, at 542,615 to 543,440
_RS02700
LU632_RS02705: LU632_RS02705 - IS6 family transposase, at 543,501 to 543,963
_RS02705
Position (kb) 541
542
543 Strain fitness (log2 ratio) -1
0
1 at 541.118 kb on + strand at 541.143 kb on + strand at 541.363 kb on + strand, within fkpB at 542.546 kb on + strand at 542.552 kb on - strand at 542.620 kb on + strand at 543.471 kb on + strand at 543.471 kb on + strand at 543.473 kb on + strand at 543.473 kb on + strand at 543.473 kb on + strand at 543.501 kb on + strand at 543.501 kb on + strand at 543.503 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Glucose remove 541,118 + +0.8 541,143 + -0.3 541,363 + fkpB LU632_RS02690 0.49 -1.0 542,546 + +0.2 542,552 - +0.5 542,620 + -0.8 543,471 + -0.8 543,471 + +0.6 543,473 + -0.4 543,473 + -0.4 543,473 + +0.3 543,501 + +0.2 543,501 + -0.0 543,503 + +0.5
Or see this region's nucleotide sequence