Experiment: HBM-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thrA and thrB are separated by 2 nucleotides thrB and thrC are separated by 3 nucleotides
LU632_RS02610: thrA - bifunctional aspartate kinase/homoserine dehydrogenase I, at 520,215 to 522,677
thrA
LU632_RS02615: thrB - homoserine kinase, at 522,680 to 523,609
thrB
LU632_RS02620: thrC - threonine synthase, at 523,613 to 524,899
thrC
Position (kb) 522
523
524 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 521.700 kb on - strand, within thrA at 521.700 kb on - strand, within thrA at 521.855 kb on + strand, within thrA at 521.905 kb on - strand, within thrA at 521.976 kb on + strand, within thrA at 521.976 kb on + strand, within thrA at 521.977 kb on - strand, within thrA at 521.977 kb on - strand, within thrA at 521.977 kb on - strand, within thrA at 521.989 kb on + strand, within thrA at 521.989 kb on + strand, within thrA at 521.989 kb on + strand, within thrA at 521.994 kb on + strand, within thrA at 522.061 kb on + strand, within thrA at 522.140 kb on + strand, within thrA at 522.141 kb on - strand, within thrA at 522.141 kb on - strand, within thrA at 522.166 kb on + strand, within thrA at 522.167 kb on - strand, within thrA at 522.435 kb on - strand at 522.519 kb on + strand at 522.519 kb on + strand at 522.650 kb on + strand at 522.651 kb on - strand at 522.723 kb on + strand at 522.735 kb on + strand at 522.787 kb on + strand, within thrB at 522.842 kb on - strand, within thrB at 522.869 kb on - strand, within thrB at 522.869 kb on - strand, within thrB at 523.016 kb on + strand, within thrB at 523.016 kb on + strand, within thrB at 523.017 kb on - strand, within thrB at 523.017 kb on - strand, within thrB at 523.219 kb on + strand, within thrB at 523.219 kb on + strand, within thrB at 523.262 kb on + strand, within thrB at 523.263 kb on - strand, within thrB at 523.263 kb on - strand, within thrB at 523.384 kb on + strand, within thrB at 523.427 kb on - strand, within thrB at 523.432 kb on + strand, within thrB at 523.499 kb on - strand, within thrB at 523.539 kb on - strand at 523.539 kb on - strand at 523.568 kb on - strand at 523.598 kb on - strand at 523.970 kb on + strand, within thrC
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Glucose remove 521,700 - thrA LU632_RS02610 0.60 -3.1 521,700 - thrA LU632_RS02610 0.60 -1.2 521,855 + thrA LU632_RS02610 0.67 -1.8 521,905 - thrA LU632_RS02610 0.69 -4.3 521,976 + thrA LU632_RS02610 0.71 -1.8 521,976 + thrA LU632_RS02610 0.71 -2.5 521,977 - thrA LU632_RS02610 0.72 -0.2 521,977 - thrA LU632_RS02610 0.72 -2.4 521,977 - thrA LU632_RS02610 0.72 -0.8 521,989 + thrA LU632_RS02610 0.72 -2.3 521,989 + thrA LU632_RS02610 0.72 -1.5 521,989 + thrA LU632_RS02610 0.72 -2.5 521,994 + thrA LU632_RS02610 0.72 -4.0 522,061 + thrA LU632_RS02610 0.75 -4.3 522,140 + thrA LU632_RS02610 0.78 -1.2 522,141 - thrA LU632_RS02610 0.78 -2.6 522,141 - thrA LU632_RS02610 0.78 -2.2 522,166 + thrA LU632_RS02610 0.79 -2.9 522,167 - thrA LU632_RS02610 0.79 -0.2 522,435 - -2.4 522,519 + -2.8 522,519 + -2.2 522,650 + -0.6 522,651 - -0.8 522,723 + -1.2 522,735 + -0.8 522,787 + thrB LU632_RS02615 0.12 -1.8 522,842 - thrB LU632_RS02615 0.17 -4.4 522,869 - thrB LU632_RS02615 0.20 -1.2 522,869 - thrB LU632_RS02615 0.20 -1.2 523,016 + thrB LU632_RS02615 0.36 -3.7 523,016 + thrB LU632_RS02615 0.36 -0.2 523,017 - thrB LU632_RS02615 0.36 -2.4 523,017 - thrB LU632_RS02615 0.36 +0.2 523,219 + thrB LU632_RS02615 0.58 -2.2 523,219 + thrB LU632_RS02615 0.58 -2.0 523,262 + thrB LU632_RS02615 0.63 -3.6 523,263 - thrB LU632_RS02615 0.63 -2.2 523,263 - thrB LU632_RS02615 0.63 -2.4 523,384 + thrB LU632_RS02615 0.76 -3.1 523,427 - thrB LU632_RS02615 0.80 -0.2 523,432 + thrB LU632_RS02615 0.81 -2.4 523,499 - thrB LU632_RS02615 0.88 -3.7 523,539 - -2.2 523,539 - -2.2 523,568 - -2.4 523,598 - -1.2 523,970 + thrC LU632_RS02620 0.28 -0.2
Or see this region's nucleotide sequence