Experiment: HBM-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mtnK and LU632_RS01125 are separated by 74 nucleotides LU632_RS01125 and LU632_RS01130 are separated by 130 nucleotides LU632_RS01130 and lpcA are separated by 213 nucleotides lpcA and LU632_RS01140 are separated by 43 nucleotides
LU632_RS01120: mtnK - S-methyl-5-thioribose kinase, at 238,847 to 240,032
mtnK
LU632_RS01125: LU632_RS01125 - IS5/IS1182 family transposase, at 240,107 to 240,743
_RS01125
LU632_RS01130: LU632_RS01130 - acyl-CoA dehydrogenase family protein, at 240,874 to 241,640
_RS01130
LU632_RS01135: lpcA - D-sedoheptulose 7-phosphate isomerase, at 241,854 to 242,432
lpcA
LU632_RS01140: LU632_RS01140 - class II glutamine amidotransferase, at 242,476 to 243,243
_RS01140
Position (kb) 240
241
242 Strain fitness (log2 ratio) -2
-1
0
1 at 239.894 kb on + strand, within mtnK at 239.896 kb on + strand, within mtnK at 239.896 kb on + strand, within mtnK at 239.896 kb on + strand, within mtnK at 239.897 kb on - strand, within mtnK at 239.897 kb on - strand, within mtnK at 239.897 kb on - strand, within mtnK at 239.984 kb on + strand at 239.985 kb on - strand at 240.084 kb on - strand at 240.084 kb on - strand at 240.119 kb on - strand at 240.841 kb on - strand at 240.841 kb on - strand at 240.989 kb on + strand, within LU632_RS01130 at 241.077 kb on + strand, within LU632_RS01130 at 241.078 kb on - strand, within LU632_RS01130 at 241.185 kb on - strand, within LU632_RS01130 at 241.303 kb on - strand, within LU632_RS01130 at 241.573 kb on - strand at 241.589 kb on - strand at 241.640 kb on + strand at 241.641 kb on - strand at 241.641 kb on - strand at 241.720 kb on + strand at 241.753 kb on - strand at 242.163 kb on + strand, within lpcA at 242.379 kb on - strand at 242.430 kb on + strand at 242.469 kb on + strand at 242.469 kb on + strand at 242.470 kb on - strand at 242.470 kb on - strand at 242.470 kb on - strand at 242.476 kb on - strand at 242.622 kb on + strand, within LU632_RS01140 at 242.623 kb on - strand, within LU632_RS01140 at 242.623 kb on - strand, within LU632_RS01140 at 242.623 kb on - strand, within LU632_RS01140 at 242.623 kb on - strand, within LU632_RS01140
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Glucose remove 239,894 + mtnK LU632_RS01120 0.88 -0.2 239,896 + mtnK LU632_RS01120 0.88 +0.6 239,896 + mtnK LU632_RS01120 0.88 +0.2 239,896 + mtnK LU632_RS01120 0.88 -0.7 239,897 - mtnK LU632_RS01120 0.89 -0.1 239,897 - mtnK LU632_RS01120 0.89 -0.2 239,897 - mtnK LU632_RS01120 0.89 +0.8 239,984 + -1.2 239,985 - -0.8 240,084 - +0.3 240,084 - -1.1 240,119 - -0.7 240,841 - -2.0 240,841 - -0.6 240,989 + LU632_RS01130 0.15 +0.4 241,077 + LU632_RS01130 0.26 +1.0 241,078 - LU632_RS01130 0.27 -0.1 241,185 - LU632_RS01130 0.41 +0.0 241,303 - LU632_RS01130 0.56 -1.9 241,573 - -0.5 241,589 - -0.4 241,640 + -0.2 241,641 - -0.7 241,641 - -0.0 241,720 + -0.1 241,753 - -0.0 242,163 + lpcA LU632_RS01135 0.53 +0.8 242,379 - -1.2 242,430 + +0.2 242,469 + -0.2 242,469 + +0.3 242,470 - -1.5 242,470 - +0.3 242,470 - -0.2 242,476 - +1.1 242,622 + LU632_RS01140 0.19 -0.0 242,623 - LU632_RS01140 0.19 -0.2 242,623 - LU632_RS01140 0.19 -0.3 242,623 - LU632_RS01140 0.19 -1.4 242,623 - LU632_RS01140 0.19 +0.8
Or see this region's nucleotide sequence