Experiment: HBM-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS00485 and LU632_RS00490 overlap by 11 nucleotides LU632_RS00490 and sctS overlap by 4 nucleotides sctS and sctT are separated by 12 nucleotides sctT and LU632_RS00505 are separated by 9 nucleotides
LU632_RS00485: LU632_RS00485 - FliM/FliN family flagellar motor switch protein, at 100,068 to 100,994
_RS00485
LU632_RS00490: LU632_RS00490 - EscR/YscR/HrcR family type III secretion system export apparatus protein, at 100,984 to 101,655
_RS00490
LU632_RS00495: sctS - type III secretion system export apparatus subunit SctS, at 101,652 to 101,906
sctS
LU632_RS00500: sctT - type III secretion system export apparatus subunit SctT, at 101,919 to 102,683
sctT
LU632_RS00505: LU632_RS00505 - EscU/YscU/HrcU family type III secretion system export apparatus switch protein, at 102,693 to 103,784
_RS00505
Position (kb)
101
102 Strain fitness (log2 ratio)
-1
0
1 at 100.751 kb on - strand, within LU632_RS00485 at 101.059 kb on - strand, within LU632_RS00490 at 101.096 kb on + strand, within LU632_RS00490 at 101.459 kb on + strand, within LU632_RS00490 at 101.750 kb on - strand, within sctS at 101.905 kb on - strand at 101.905 kb on - strand at 101.938 kb on - strand at 101.955 kb on - strand at 102.007 kb on + strand, within sctT at 102.010 kb on + strand, within sctT at 102.050 kb on - strand, within sctT at 102.110 kb on + strand, within sctT at 102.345 kb on - strand, within sctT at 102.348 kb on + strand, within sctT at 102.349 kb on - strand, within sctT at 102.349 kb on - strand, within sctT at 102.352 kb on + strand, within sctT at 102.465 kb on - strand, within sctT at 102.465 kb on - strand, within sctT at 102.467 kb on - strand, within sctT
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Glucose remove 100,751 - LU632_RS00485 0.74 +0.4 101,059 - LU632_RS00490 0.11 +0.2 101,096 + LU632_RS00490 0.17 -0.2 101,459 + LU632_RS00490 0.71 -0.6 101,750 - sctS LU632_RS00495 0.38 +0.6 101,905 - +0.9 101,905 - -0.3 101,938 - +0.0 101,955 - +0.4 102,007 + sctT LU632_RS00500 0.12 -1.0 102,010 + sctT LU632_RS00500 0.12 -0.9 102,050 - sctT LU632_RS00500 0.17 +0.1 102,110 + sctT LU632_RS00500 0.25 -0.3 102,345 - sctT LU632_RS00500 0.56 +0.4 102,348 + sctT LU632_RS00500 0.56 +0.3 102,349 - sctT LU632_RS00500 0.56 +1.5 102,349 - sctT LU632_RS00500 0.56 +0.8 102,352 + sctT LU632_RS00500 0.57 +0.6 102,465 - sctT LU632_RS00500 0.71 -1.0 102,465 - sctT LU632_RS00500 0.71 -0.5 102,467 - sctT LU632_RS00500 0.72 -0.3
Or see this region's nucleotide sequence