Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fkpA and LU632_RS22720 are separated by 286 nucleotides LU632_RS22720 and slyD are separated by 130 nucleotides slyD and LU632_RS22730 are separated by 136 nucleotides LU632_RS22730 and kefB are separated by 8 nucleotides
LU632_RS22715: fkpA - FKBP-type peptidyl-prolyl cis-trans isomerase, at 4,324,632 to 4,325,471
fkpA
LU632_RS22720: LU632_RS22720 - SlyX family protein, at 4,325,758 to 4,325,973
_RS22720
LU632_RS22725: slyD - peptidylprolyl isomerase, at 4,326,104 to 4,326,625
slyD
LU632_RS22730: LU632_RS22730 - YheV family putative metal-binding protein, at 4,326,762 to 4,326,956
_RS22730
LU632_RS22735: kefB - glutathione-regulated potassium-efflux system protein KefB, at 4,326,965 to 4,328,770
kefB
Position (kb) 4326
4327 Strain fitness (log2 ratio) -1
0
1
2 at 4325.272 kb on + strand, within fkpA at 4325.272 kb on + strand, within fkpA at 4325.451 kb on - strand at 4325.529 kb on + strand at 4325.581 kb on + strand at 4325.581 kb on + strand at 4325.650 kb on - strand at 4325.686 kb on - strand at 4325.847 kb on + strand, within LU632_RS22720 at 4325.847 kb on + strand, within LU632_RS22720 at 4325.847 kb on + strand, within LU632_RS22720 at 4325.848 kb on - strand, within LU632_RS22720 at 4325.888 kb on + strand, within LU632_RS22720 at 4325.968 kb on + strand at 4325.969 kb on - strand at 4325.972 kb on - strand at 4325.972 kb on - strand at 4326.016 kb on - strand at 4326.236 kb on - strand, within slyD at 4326.236 kb on - strand, within slyD at 4326.299 kb on + strand, within slyD at 4326.423 kb on + strand, within slyD at 4326.497 kb on + strand, within slyD at 4326.498 kb on - strand, within slyD at 4326.543 kb on - strand, within slyD at 4326.577 kb on + strand at 4326.589 kb on - strand at 4326.629 kb on + strand at 4326.630 kb on - strand at 4326.730 kb on - strand at 4327.108 kb on + strand at 4327.108 kb on + strand at 4327.108 kb on + strand at 4327.108 kb on + strand at 4327.108 kb on + strand at 4327.108 kb on + strand at 4327.108 kb on + strand at 4327.108 kb on + strand at 4327.108 kb on + strand at 4327.108 kb on + strand at 4327.108 kb on + strand at 4327.109 kb on - strand at 4327.109 kb on - strand at 4327.109 kb on - strand at 4327.109 kb on - strand at 4327.109 kb on - strand at 4327.351 kb on + strand, within kefB at 4327.401 kb on - strand, within kefB at 4327.441 kb on - strand, within kefB at 4327.446 kb on + strand, within kefB at 4327.451 kb on - strand, within kefB at 4327.451 kb on - strand, within kefB at 4327.496 kb on - strand, within kefB at 4327.496 kb on - strand, within kefB at 4327.496 kb on - strand, within kefB at 4327.602 kb on + strand, within kefB
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 4,325,272 + fkpA LU632_RS22715 0.76 -0.4 4,325,272 + fkpA LU632_RS22715 0.76 +0.3 4,325,451 - -0.6 4,325,529 + -0.3 4,325,581 + -0.4 4,325,581 + -0.1 4,325,650 - +0.0 4,325,686 - -0.3 4,325,847 + LU632_RS22720 0.41 +0.3 4,325,847 + LU632_RS22720 0.41 +0.1 4,325,847 + LU632_RS22720 0.41 +0.4 4,325,848 - LU632_RS22720 0.42 -0.2 4,325,888 + LU632_RS22720 0.60 +0.7 4,325,968 + +0.2 4,325,969 - +0.4 4,325,972 - +0.7 4,325,972 - -0.1 4,326,016 - +0.3 4,326,236 - slyD LU632_RS22725 0.25 +1.0 4,326,236 - slyD LU632_RS22725 0.25 -1.7 4,326,299 + slyD LU632_RS22725 0.37 +0.1 4,326,423 + slyD LU632_RS22725 0.61 -0.3 4,326,497 + slyD LU632_RS22725 0.75 -0.3 4,326,498 - slyD LU632_RS22725 0.75 -0.8 4,326,543 - slyD LU632_RS22725 0.84 +0.1 4,326,577 + -0.1 4,326,589 - -0.0 4,326,629 + +0.4 4,326,630 - +0.7 4,326,730 - +0.1 4,327,108 + +1.1 4,327,108 + +0.1 4,327,108 + +0.7 4,327,108 + -0.0 4,327,108 + +1.6 4,327,108 + +0.5 4,327,108 + +0.0 4,327,108 + -1.3 4,327,108 + +0.3 4,327,108 + +0.2 4,327,108 + +0.4 4,327,109 - +0.3 4,327,109 - -0.1 4,327,109 - -0.3 4,327,109 - +0.4 4,327,109 - +0.9 4,327,351 + kefB LU632_RS22735 0.21 +0.1 4,327,401 - kefB LU632_RS22735 0.24 +2.0 4,327,441 - kefB LU632_RS22735 0.26 -0.1 4,327,446 + kefB LU632_RS22735 0.27 +0.0 4,327,451 - kefB LU632_RS22735 0.27 +0.0 4,327,451 - kefB LU632_RS22735 0.27 -0.3 4,327,496 - kefB LU632_RS22735 0.29 -0.7 4,327,496 - kefB LU632_RS22735 0.29 -0.2 4,327,496 - kefB LU632_RS22735 0.29 +1.4 4,327,602 + kefB LU632_RS22735 0.35 +0.4
Or see this region's nucleotide sequence