Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aaeB and aaeA are separated by 4 nucleotides aaeA and LU632_RS21410 are separated by 7 nucleotides LU632_RS21410 and aaeR are separated by 263 nucleotides
LU632_RS21400: aaeB - p-hydroxybenzoic acid efflux pump subunit AaeB, at 4,089,069 to 4,091,027
aaeB
LU632_RS21405: aaeA - p-hydroxybenzoic acid efflux pump subunit AaeA, at 4,091,032 to 4,091,964
aaeA
LU632_RS21410: LU632_RS21410 - p-hydroxybenzoic acid efflux pump operon protein AaeX, at 4,091,972 to 4,092,175
_RS21410
LU632_RS21415: aaeR - HTH-type transcriptional activator AaeR, at 4,092,439 to 4,093,357
aaeR
Position (kb)
4091
4092
4093 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4091.018 kb on - strand at 4091.018 kb on - strand at 4091.065 kb on + strand at 4091.066 kb on - strand at 4091.173 kb on - strand, within aaeA at 4091.241 kb on + strand, within aaeA at 4091.292 kb on + strand, within aaeA at 4091.297 kb on + strand, within aaeA at 4091.298 kb on - strand, within aaeA at 4091.378 kb on + strand, within aaeA at 4091.378 kb on + strand, within aaeA at 4091.378 kb on + strand, within aaeA at 4091.379 kb on - strand, within aaeA at 4091.379 kb on - strand, within aaeA at 4091.391 kb on - strand, within aaeA at 4091.608 kb on + strand, within aaeA at 4091.610 kb on + strand, within aaeA at 4091.678 kb on + strand, within aaeA at 4091.678 kb on + strand, within aaeA at 4091.871 kb on - strand at 4091.950 kb on - strand at 4092.055 kb on - strand, within LU632_RS21410 at 4092.150 kb on - strand, within LU632_RS21410 at 4092.250 kb on + strand at 4092.251 kb on - strand at 4092.308 kb on + strand at 4092.308 kb on + strand at 4092.317 kb on - strand at 4092.319 kb on + strand at 4092.319 kb on + strand at 4092.319 kb on + strand at 4092.319 kb on + strand at 4092.320 kb on - strand at 4092.320 kb on - strand at 4092.352 kb on + strand at 4092.352 kb on + strand at 4092.398 kb on + strand at 4092.402 kb on + strand at 4092.413 kb on - strand at 4092.492 kb on + strand at 4092.495 kb on + strand at 4092.496 kb on - strand at 4092.496 kb on - strand at 4092.515 kb on + strand at 4092.522 kb on - strand at 4092.581 kb on + strand, within aaeR at 4092.585 kb on + strand, within aaeR at 4092.591 kb on + strand, within aaeR at 4092.595 kb on - strand, within aaeR at 4092.595 kb on - strand, within aaeR at 4092.595 kb on - strand, within aaeR at 4092.699 kb on + strand, within aaeR at 4092.702 kb on + strand, within aaeR at 4092.860 kb on + strand, within aaeR at 4092.860 kb on + strand, within aaeR at 4092.861 kb on - strand, within aaeR at 4092.861 kb on - strand, within aaeR at 4092.890 kb on - strand, within aaeR at 4092.899 kb on - strand, within aaeR at 4092.950 kb on - strand, within aaeR at 4093.098 kb on + strand, within aaeR
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 4,091,018 - +0.4 4,091,018 - +0.8 4,091,065 + +0.1 4,091,066 - -0.0 4,091,173 - aaeA LU632_RS21405 0.15 -0.2 4,091,241 + aaeA LU632_RS21405 0.22 -0.8 4,091,292 + aaeA LU632_RS21405 0.28 +0.0 4,091,297 + aaeA LU632_RS21405 0.28 +0.4 4,091,298 - aaeA LU632_RS21405 0.29 -0.6 4,091,378 + aaeA LU632_RS21405 0.37 +0.9 4,091,378 + aaeA LU632_RS21405 0.37 -1.0 4,091,378 + aaeA LU632_RS21405 0.37 -0.2 4,091,379 - aaeA LU632_RS21405 0.37 +0.2 4,091,379 - aaeA LU632_RS21405 0.37 -0.4 4,091,391 - aaeA LU632_RS21405 0.38 +0.2 4,091,608 + aaeA LU632_RS21405 0.62 +0.1 4,091,610 + aaeA LU632_RS21405 0.62 +0.0 4,091,678 + aaeA LU632_RS21405 0.69 +0.9 4,091,678 + aaeA LU632_RS21405 0.69 +0.1 4,091,871 - +0.1 4,091,950 - -1.8 4,092,055 - LU632_RS21410 0.41 +3.2 4,092,150 - LU632_RS21410 0.87 +0.0 4,092,250 + -0.6 4,092,251 - +0.9 4,092,308 + -0.1 4,092,308 + -0.2 4,092,317 - -0.2 4,092,319 + +1.2 4,092,319 + +0.2 4,092,319 + -1.3 4,092,319 + +0.6 4,092,320 - +0.3 4,092,320 - +0.1 4,092,352 + +0.6 4,092,352 + +0.4 4,092,398 + +0.6 4,092,402 + -1.2 4,092,413 - +1.5 4,092,492 + +0.9 4,092,495 + -0.2 4,092,496 - -2.8 4,092,496 - +0.3 4,092,515 + -0.0 4,092,522 - -0.4 4,092,581 + aaeR LU632_RS21415 0.15 +1.4 4,092,585 + aaeR LU632_RS21415 0.16 -0.2 4,092,591 + aaeR LU632_RS21415 0.17 +0.2 4,092,595 - aaeR LU632_RS21415 0.17 -0.1 4,092,595 - aaeR LU632_RS21415 0.17 -0.2 4,092,595 - aaeR LU632_RS21415 0.17 +1.2 4,092,699 + aaeR LU632_RS21415 0.28 -0.1 4,092,702 + aaeR LU632_RS21415 0.29 -0.8 4,092,860 + aaeR LU632_RS21415 0.46 -1.3 4,092,860 + aaeR LU632_RS21415 0.46 +0.8 4,092,861 - aaeR LU632_RS21415 0.46 -1.5 4,092,861 - aaeR LU632_RS21415 0.46 -0.8 4,092,890 - aaeR LU632_RS21415 0.49 -0.0 4,092,899 - aaeR LU632_RS21415 0.50 +1.9 4,092,950 - aaeR LU632_RS21415 0.56 -0.4 4,093,098 + aaeR LU632_RS21415 0.72 +2.5
Or see this region's nucleotide sequence