Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt panB and panC are separated by 15 nucleotides panC and panD are separated by 69 nucleotides panD and tnpA are separated by 68 nucleotides tnpA and LU632_RS20120 are separated by 235 nucleotides
LU632_RS20100: panB - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 3,828,991 to 3,829,785
panB
LU632_RS20105: panC - pantoate--beta-alanine ligase, at 3,829,801 to 3,830,655
panC
LU632_RS20110: panD - aspartate 1-decarboxylase, at 3,830,725 to 3,831,105
panD
LU632_RS20115: tnpA - IS200/IS605 family transposase, at 3,831,174 to 3,831,620
tnpA
LU632_RS20120: LU632_RS20120 - ABC transporter permease, at 3,831,856 to 3,832,626
_RS20120
Position (kb)
3830
3831
3832 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3829.729 kb on + strand at 3829.729 kb on + strand at 3829.730 kb on - strand at 3829.781 kb on - strand at 3829.781 kb on - strand at 3829.869 kb on + strand at 3829.912 kb on - strand, within panC at 3829.912 kb on - strand, within panC at 3829.957 kb on - strand, within panC at 3830.126 kb on + strand, within panC at 3830.257 kb on + strand, within panC at 3830.302 kb on + strand, within panC at 3830.375 kb on - strand, within panC at 3830.472 kb on + strand, within panC at 3830.629 kb on - strand at 3830.789 kb on - strand, within panD at 3830.845 kb on + strand, within panD at 3830.846 kb on - strand, within panD at 3830.859 kb on + strand, within panD at 3830.860 kb on - strand, within panD at 3830.863 kb on + strand, within panD at 3830.863 kb on + strand, within panD at 3830.864 kb on + strand, within panD at 3830.864 kb on - strand, within panD at 3830.864 kb on - strand, within panD at 3830.896 kb on + strand, within panD at 3830.897 kb on - strand, within panD at 3830.897 kb on - strand, within panD at 3830.901 kb on + strand, within panD at 3830.901 kb on + strand, within panD at 3830.979 kb on - strand, within panD at 3830.983 kb on - strand, within panD at 3831.034 kb on - strand, within panD at 3831.765 kb on + strand at 3831.854 kb on + strand at 3831.905 kb on + strand at 3831.905 kb on + strand at 3831.905 kb on + strand at 3831.943 kb on + strand, within LU632_RS20120
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 3,829,729 + -2.9 3,829,729 + -1.5 3,829,730 - -1.3 3,829,781 - -3.0 3,829,781 - +2.1 3,829,869 + -1.7 3,829,912 - panC LU632_RS20105 0.13 -1.2 3,829,912 - panC LU632_RS20105 0.13 -0.0 3,829,957 - panC LU632_RS20105 0.18 -1.3 3,830,126 + panC LU632_RS20105 0.38 -1.8 3,830,257 + panC LU632_RS20105 0.53 -0.9 3,830,302 + panC LU632_RS20105 0.59 -2.4 3,830,375 - panC LU632_RS20105 0.67 -2.1 3,830,472 + panC LU632_RS20105 0.78 -0.9 3,830,629 - -1.9 3,830,789 - panD LU632_RS20110 0.17 -0.5 3,830,845 + panD LU632_RS20110 0.31 -1.5 3,830,846 - panD LU632_RS20110 0.32 +0.1 3,830,859 + panD LU632_RS20110 0.35 -0.9 3,830,860 - panD LU632_RS20110 0.35 -1.7 3,830,863 + panD LU632_RS20110 0.36 -0.8 3,830,863 + panD LU632_RS20110 0.36 -0.5 3,830,864 + panD LU632_RS20110 0.36 -1.7 3,830,864 - panD LU632_RS20110 0.36 -0.6 3,830,864 - panD LU632_RS20110 0.36 -2.0 3,830,896 + panD LU632_RS20110 0.45 -0.6 3,830,897 - panD LU632_RS20110 0.45 -0.5 3,830,897 - panD LU632_RS20110 0.45 -1.1 3,830,901 + panD LU632_RS20110 0.46 -1.5 3,830,901 + panD LU632_RS20110 0.46 +1.7 3,830,979 - panD LU632_RS20110 0.67 -2.5 3,830,983 - panD LU632_RS20110 0.68 -3.7 3,831,034 - panD LU632_RS20110 0.81 -1.8 3,831,765 + +0.2 3,831,854 + +0.8 3,831,905 + +0.4 3,831,905 + +1.7 3,831,905 + -0.2 3,831,943 + LU632_RS20120 0.11 -1.7
Or see this region's nucleotide sequence