Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pcnB and folK overlap by 4 nucleotides folK and panB are separated by 60 nucleotides panB and panC are separated by 15 nucleotides panC and panD are separated by 69 nucleotides
LU632_RS20090: pcnB - polynucleotide adenylyltransferase PcnB, at 3,827,039 to 3,828,454
pcnB
LU632_RS20095: folK - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase, at 3,828,451 to 3,828,930
folK
LU632_RS20100: panB - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 3,828,991 to 3,829,785
panB
LU632_RS20105: panC - pantoate--beta-alanine ligase, at 3,829,801 to 3,830,655
panC
LU632_RS20110: panD - aspartate 1-decarboxylase, at 3,830,725 to 3,831,105
panD
Position (kb)
3828
3829
3830 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3828.216 kb on + strand, within pcnB at 3828.958 kb on + strand at 3828.958 kb on + strand at 3828.958 kb on + strand at 3828.959 kb on - strand at 3828.974 kb on + strand at 3829.063 kb on + strand at 3829.072 kb on - strand, within panB at 3829.189 kb on + strand, within panB at 3829.189 kb on + strand, within panB at 3829.259 kb on - strand, within panB at 3829.300 kb on - strand, within panB at 3829.317 kb on + strand, within panB at 3829.318 kb on - strand, within panB at 3829.536 kb on + strand, within panB at 3829.574 kb on + strand, within panB at 3829.575 kb on - strand, within panB at 3829.575 kb on - strand, within panB at 3829.575 kb on - strand, within panB at 3829.579 kb on - strand, within panB at 3829.729 kb on + strand at 3829.729 kb on + strand at 3829.730 kb on - strand at 3829.781 kb on - strand at 3829.781 kb on - strand at 3829.869 kb on + strand at 3829.912 kb on - strand, within panC at 3829.912 kb on - strand, within panC at 3829.957 kb on - strand, within panC at 3830.126 kb on + strand, within panC at 3830.257 kb on + strand, within panC at 3830.302 kb on + strand, within panC at 3830.375 kb on - strand, within panC at 3830.472 kb on + strand, within panC at 3830.629 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 3,828,216 + pcnB LU632_RS20090 0.83 +0.4 3,828,958 + +0.1 3,828,958 + -0.1 3,828,958 + -0.8 3,828,959 - -1.1 3,828,974 + -0.3 3,829,063 + -0.9 3,829,072 - panB LU632_RS20100 0.10 +0.5 3,829,189 + panB LU632_RS20100 0.25 -1.0 3,829,189 + panB LU632_RS20100 0.25 -0.3 3,829,259 - panB LU632_RS20100 0.34 -2.0 3,829,300 - panB LU632_RS20100 0.39 -2.1 3,829,317 + panB LU632_RS20100 0.41 -1.0 3,829,318 - panB LU632_RS20100 0.41 -1.2 3,829,536 + panB LU632_RS20100 0.69 -1.0 3,829,574 + panB LU632_RS20100 0.73 -2.8 3,829,575 - panB LU632_RS20100 0.73 -2.5 3,829,575 - panB LU632_RS20100 0.73 -0.3 3,829,575 - panB LU632_RS20100 0.73 -1.9 3,829,579 - panB LU632_RS20100 0.74 -1.8 3,829,729 + -2.9 3,829,729 + -1.5 3,829,730 - -1.3 3,829,781 - -3.0 3,829,781 - +2.1 3,829,869 + -1.7 3,829,912 - panC LU632_RS20105 0.13 -1.2 3,829,912 - panC LU632_RS20105 0.13 -0.0 3,829,957 - panC LU632_RS20105 0.18 -1.3 3,830,126 + panC LU632_RS20105 0.38 -1.8 3,830,257 + panC LU632_RS20105 0.53 -0.9 3,830,302 + panC LU632_RS20105 0.59 -2.4 3,830,375 - panC LU632_RS20105 0.67 -2.1 3,830,472 + panC LU632_RS20105 0.78 -0.9 3,830,629 - -1.9
Or see this region's nucleotide sequence