Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tnpA and zapB are separated by 161 nucleotides zapB and LU632_RS19700 are separated by 434 nucleotides LU632_RS19700 and glpK are separated by 32 nucleotides
LU632_RS19690: tnpA - IS200/IS605 family transposase, at 3,740,746 to 3,741,183
tnpA
LU632_RS19695: zapB - cell division protein ZapB, at 3,741,345 to 3,741,584
zapB
LU632_RS19700: LU632_RS19700 - aquaporin family protein, at 3,742,019 to 3,742,867
_RS19700
LU632_RS19705: glpK - glycerol kinase GlpK, at 3,742,900 to 3,744,414
glpK
Position (kb)
3742
3743 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3741.429 kb on + strand, within zapB at 3741.429 kb on + strand, within zapB at 3741.430 kb on - strand, within zapB at 3741.531 kb on - strand, within zapB at 3741.551 kb on + strand, within zapB at 3741.590 kb on + strand at 3741.591 kb on - strand at 3741.670 kb on + strand at 3741.670 kb on + strand at 3741.670 kb on + strand at 3741.670 kb on + strand at 3741.671 kb on - strand at 3741.671 kb on - strand at 3741.674 kb on + strand at 3741.675 kb on - strand at 3741.769 kb on + strand at 3741.948 kb on - strand at 3741.993 kb on + strand at 3742.084 kb on + strand at 3742.085 kb on - strand at 3742.160 kb on - strand, within LU632_RS19700 at 3742.190 kb on + strand, within LU632_RS19700 at 3742.190 kb on + strand, within LU632_RS19700 at 3742.286 kb on + strand, within LU632_RS19700 at 3742.286 kb on + strand, within LU632_RS19700 at 3742.286 kb on + strand, within LU632_RS19700 at 3742.286 kb on + strand, within LU632_RS19700 at 3742.287 kb on - strand, within LU632_RS19700 at 3742.287 kb on - strand, within LU632_RS19700 at 3742.288 kb on + strand, within LU632_RS19700 at 3742.433 kb on + strand, within LU632_RS19700 at 3742.456 kb on - strand, within LU632_RS19700 at 3742.693 kb on + strand, within LU632_RS19700 at 3742.709 kb on - strand, within LU632_RS19700 at 3742.777 kb on + strand, within LU632_RS19700 at 3742.777 kb on + strand, within LU632_RS19700 at 3742.777 kb on + strand, within LU632_RS19700 at 3742.778 kb on - strand, within LU632_RS19700 at 3742.778 kb on - strand, within LU632_RS19700 at 3742.778 kb on - strand, within LU632_RS19700 at 3742.858 kb on + strand at 3742.859 kb on - strand at 3742.866 kb on - strand at 3743.197 kb on + strand, within glpK at 3743.273 kb on - strand, within glpK at 3743.275 kb on - strand, within glpK at 3743.527 kb on - strand, within glpK at 3743.699 kb on - strand, within glpK at 3743.715 kb on + strand, within glpK at 3743.728 kb on - strand, within glpK at 3743.804 kb on - strand, within glpK at 3743.804 kb on - strand, within glpK
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 3,741,429 + zapB LU632_RS19695 0.35 +0.0 3,741,429 + zapB LU632_RS19695 0.35 -0.5 3,741,430 - zapB LU632_RS19695 0.35 -0.0 3,741,531 - zapB LU632_RS19695 0.78 -0.5 3,741,551 + zapB LU632_RS19695 0.86 -0.3 3,741,590 + +0.1 3,741,591 - +0.4 3,741,670 + -0.1 3,741,670 + -1.2 3,741,670 + +0.3 3,741,670 + +0.4 3,741,671 - +0.4 3,741,671 - -0.3 3,741,674 + +0.2 3,741,675 - -1.0 3,741,769 + -0.4 3,741,948 - +0.4 3,741,993 + +0.5 3,742,084 + -0.6 3,742,085 - +1.1 3,742,160 - LU632_RS19700 0.17 +0.1 3,742,190 + LU632_RS19700 0.20 +0.1 3,742,190 + LU632_RS19700 0.20 -0.9 3,742,286 + LU632_RS19700 0.31 +0.7 3,742,286 + LU632_RS19700 0.31 +0.4 3,742,286 + LU632_RS19700 0.31 +0.2 3,742,286 + LU632_RS19700 0.31 -0.5 3,742,287 - LU632_RS19700 0.32 -0.8 3,742,287 - LU632_RS19700 0.32 +0.2 3,742,288 + LU632_RS19700 0.32 -0.0 3,742,433 + LU632_RS19700 0.49 +0.2 3,742,456 - LU632_RS19700 0.51 -1.1 3,742,693 + LU632_RS19700 0.79 +3.1 3,742,709 - LU632_RS19700 0.81 -0.9 3,742,777 + LU632_RS19700 0.89 -0.2 3,742,777 + LU632_RS19700 0.89 -0.4 3,742,777 + LU632_RS19700 0.89 -0.5 3,742,778 - LU632_RS19700 0.89 -1.2 3,742,778 - LU632_RS19700 0.89 +0.1 3,742,778 - LU632_RS19700 0.89 +0.5 3,742,858 + +1.1 3,742,859 - -0.4 3,742,866 - -0.5 3,743,197 + glpK LU632_RS19705 0.20 -0.9 3,743,273 - glpK LU632_RS19705 0.25 +2.1 3,743,275 - glpK LU632_RS19705 0.25 -1.2 3,743,527 - glpK LU632_RS19705 0.41 -0.9 3,743,699 - glpK LU632_RS19705 0.53 +2.1 3,743,715 + glpK LU632_RS19705 0.54 +1.1 3,743,728 - glpK LU632_RS19705 0.55 -0.3 3,743,804 - glpK LU632_RS19705 0.60 +0.1 3,743,804 - glpK LU632_RS19705 0.60 -1.0
Or see this region's nucleotide sequence