Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS14235 and LU632_RS14240 overlap by 4 nucleotides LU632_RS14240 and LU632_RS14245 are separated by 18 nucleotides LU632_RS14245 and LU632_RS14250 are separated by 111 nucleotides LU632_RS14250 and LU632_RS14255 are separated by 102 nucleotides
LU632_RS14235: LU632_RS14235 - helix-turn-helix domain-containing protein, at 2,727,928 to 2,728,304
_RS14235
LU632_RS14240: LU632_RS14240 - phage antirepressor KilAC domain-containing protein, at 2,728,301 to 2,729,002
_RS14240
LU632_RS14245: LU632_RS14245 - lambda phage CII family protein, at 2,729,021 to 2,729,326
_RS14245
LU632_RS14250: LU632_RS14250 - helix-turn-helix domain-containing protein, at 2,729,438 to 2,729,665
_RS14250
LU632_RS14255: LU632_RS14255 - helix-turn-helix transcriptional regulator, at 2,729,768 to 2,730,499
_RS14255
Position (kb)
2729
2730 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2728.243 kb on - strand, within LU632_RS14235 at 2728.584 kb on + strand, within LU632_RS14240 at 2728.584 kb on + strand, within LU632_RS14240 at 2728.835 kb on + strand, within LU632_RS14240 at 2728.835 kb on + strand, within LU632_RS14240 at 2728.848 kb on + strand, within LU632_RS14240 at 2728.966 kb on + strand at 2729.007 kb on - strand at 2729.007 kb on - strand at 2729.310 kb on + strand at 2729.377 kb on - strand at 2729.576 kb on - strand, within LU632_RS14250 at 2729.576 kb on - strand, within LU632_RS14250
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 2,728,243 - LU632_RS14235 0.84 +0.3 2,728,584 + LU632_RS14240 0.40 +0.6 2,728,584 + LU632_RS14240 0.40 -0.2 2,728,835 + LU632_RS14240 0.76 -1.9 2,728,835 + LU632_RS14240 0.76 +0.7 2,728,848 + LU632_RS14240 0.78 +0.4 2,728,966 + +0.5 2,729,007 - -0.2 2,729,007 - -0.1 2,729,310 + +0.2 2,729,377 - -0.1 2,729,576 - LU632_RS14250 0.61 +1.9 2,729,576 - LU632_RS14250 0.61 -1.0
Or see this region's nucleotide sequence