Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliD and fliS are separated by 13 nucleotides fliS and fliT are separated by 0 nucleotides fliT and amyA are separated by 133 nucleotides amyA and LU632_RS13860 are separated by 23 nucleotides
LU632_RS13840: fliD - flagellar filament capping protein FliD, at 2,656,508 to 2,657,920
fliD
LU632_RS13845: fliS - flagellar export chaperone FliS, at 2,657,934 to 2,658,341
fliS
LU632_RS13850: fliT - flagella biosynthesis regulatory protein FliT, at 2,658,342 to 2,658,710
fliT
LU632_RS13855: amyA - alpha-amylase, at 2,658,844 to 2,659,646
amyA
LU632_RS13860: LU632_RS13860 - IS5 family transposase, at 2,659,670 to 2,660,514
_RS13860
Position (kb)
2658
2659 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2657.345 kb on + strand, within fliD at 2657.345 kb on + strand, within fliD at 2657.346 kb on - strand, within fliD at 2657.347 kb on + strand, within fliD at 2657.384 kb on + strand, within fliD at 2657.384 kb on + strand, within fliD at 2657.394 kb on + strand, within fliD at 2657.394 kb on + strand, within fliD at 2657.394 kb on + strand, within fliD at 2657.405 kb on - strand, within fliD at 2657.461 kb on + strand, within fliD at 2657.515 kb on + strand, within fliD at 2657.668 kb on + strand, within fliD at 2657.677 kb on + strand, within fliD at 2657.807 kb on + strand at 2657.863 kb on + strand at 2657.863 kb on + strand at 2657.937 kb on + strand at 2657.937 kb on + strand at 2658.198 kb on + strand, within fliS at 2658.339 kb on + strand at 2658.362 kb on - strand at 2658.533 kb on - strand, within fliT at 2658.648 kb on - strand, within fliT at 2658.657 kb on + strand, within fliT at 2658.658 kb on - strand, within fliT at 2658.659 kb on + strand, within fliT at 2658.763 kb on + strand at 2658.880 kb on + strand at 2658.880 kb on + strand at 2658.976 kb on + strand, within amyA at 2658.979 kb on - strand, within amyA at 2658.998 kb on - strand, within amyA at 2659.015 kb on - strand, within amyA at 2659.018 kb on + strand, within amyA at 2659.019 kb on - strand, within amyA at 2659.019 kb on - strand, within amyA at 2659.019 kb on - strand, within amyA at 2659.019 kb on - strand, within amyA at 2659.072 kb on + strand, within amyA at 2659.073 kb on - strand, within amyA at 2659.073 kb on - strand, within amyA at 2659.073 kb on - strand, within amyA at 2659.296 kb on + strand, within amyA at 2659.357 kb on + strand, within amyA at 2659.358 kb on - strand, within amyA at 2659.360 kb on + strand, within amyA at 2659.360 kb on + strand, within amyA at 2659.361 kb on - strand, within amyA at 2659.361 kb on - strand, within amyA at 2659.363 kb on - strand, within amyA at 2659.565 kb on - strand, within amyA at 2659.572 kb on - strand at 2659.572 kb on - strand at 2659.601 kb on + strand at 2659.649 kb on + strand at 2659.650 kb on - strand at 2659.650 kb on - strand at 2659.652 kb on - strand at 2659.652 kb on - strand at 2659.672 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 2,657,345 + fliD LU632_RS13840 0.59 +1.2 2,657,345 + fliD LU632_RS13840 0.59 -0.5 2,657,346 - fliD LU632_RS13840 0.59 -0.2 2,657,347 + fliD LU632_RS13840 0.59 -1.3 2,657,384 + fliD LU632_RS13840 0.62 -0.9 2,657,384 + fliD LU632_RS13840 0.62 -0.7 2,657,394 + fliD LU632_RS13840 0.63 -1.7 2,657,394 + fliD LU632_RS13840 0.63 -0.6 2,657,394 + fliD LU632_RS13840 0.63 -1.0 2,657,405 - fliD LU632_RS13840 0.63 -0.2 2,657,461 + fliD LU632_RS13840 0.67 +0.1 2,657,515 + fliD LU632_RS13840 0.71 +0.4 2,657,668 + fliD LU632_RS13840 0.82 +0.0 2,657,677 + fliD LU632_RS13840 0.83 +0.6 2,657,807 + +0.1 2,657,863 + +0.5 2,657,863 + +1.0 2,657,937 + +1.9 2,657,937 + +0.9 2,658,198 + fliS LU632_RS13845 0.65 -0.5 2,658,339 + -2.5 2,658,362 - -0.2 2,658,533 - fliT LU632_RS13850 0.52 -0.9 2,658,648 - fliT LU632_RS13850 0.83 -0.4 2,658,657 + fliT LU632_RS13850 0.85 -0.6 2,658,658 - fliT LU632_RS13850 0.86 -0.3 2,658,659 + fliT LU632_RS13850 0.86 +0.1 2,658,763 + +0.5 2,658,880 + +0.3 2,658,880 + -0.1 2,658,976 + amyA LU632_RS13855 0.16 +0.2 2,658,979 - amyA LU632_RS13855 0.17 +0.8 2,658,998 - amyA LU632_RS13855 0.19 -0.5 2,659,015 - amyA LU632_RS13855 0.21 +0.3 2,659,018 + amyA LU632_RS13855 0.22 -0.1 2,659,019 - amyA LU632_RS13855 0.22 -0.1 2,659,019 - amyA LU632_RS13855 0.22 -1.1 2,659,019 - amyA LU632_RS13855 0.22 -2.5 2,659,019 - amyA LU632_RS13855 0.22 +0.1 2,659,072 + amyA LU632_RS13855 0.28 -0.4 2,659,073 - amyA LU632_RS13855 0.29 -0.3 2,659,073 - amyA LU632_RS13855 0.29 +0.9 2,659,073 - amyA LU632_RS13855 0.29 +0.5 2,659,296 + amyA LU632_RS13855 0.56 +0.1 2,659,357 + amyA LU632_RS13855 0.64 +0.1 2,659,358 - amyA LU632_RS13855 0.64 +0.9 2,659,360 + amyA LU632_RS13855 0.64 +0.1 2,659,360 + amyA LU632_RS13855 0.64 +0.2 2,659,361 - amyA LU632_RS13855 0.64 -0.1 2,659,361 - amyA LU632_RS13855 0.64 +0.6 2,659,363 - amyA LU632_RS13855 0.65 -0.1 2,659,565 - amyA LU632_RS13855 0.90 +0.9 2,659,572 - +0.2 2,659,572 - +0.7 2,659,601 + +0.6 2,659,649 + -0.6 2,659,650 - -1.5 2,659,650 - +0.7 2,659,652 - +0.8 2,659,652 - +0.1 2,659,672 - +1.1
Or see this region's nucleotide sequence