Experiment: HBM-Sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS13835 and fliD are separated by 280 nucleotides fliD and fliS are separated by 13 nucleotides fliS and fliT are separated by 0 nucleotides fliT and amyA are separated by 133 nucleotides
LU632_RS13835: LU632_RS13835 - FliC/FljB family flagellin, at 2,654,944 to 2,656,227
_RS13835
LU632_RS13840: fliD - flagellar filament capping protein FliD, at 2,656,508 to 2,657,920
fliD
LU632_RS13845: fliS - flagellar export chaperone FliS, at 2,657,934 to 2,658,341
fliS
LU632_RS13850: fliT - flagella biosynthesis regulatory protein FliT, at 2,658,342 to 2,658,710
fliT
LU632_RS13855: amyA - alpha-amylase, at 2,658,844 to 2,659,646
amyA
Position (kb)
2656
2657
2658 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2655.534 kb on - strand, within LU632_RS13835 at 2655.534 kb on - strand, within LU632_RS13835 at 2655.662 kb on - strand, within LU632_RS13835 at 2655.662 kb on - strand, within LU632_RS13835 at 2655.962 kb on - strand, within LU632_RS13835 at 2655.967 kb on - strand, within LU632_RS13835 at 2655.967 kb on - strand, within LU632_RS13835 at 2656.015 kb on - strand, within LU632_RS13835 at 2656.230 kb on + strand at 2656.259 kb on + strand at 2656.388 kb on + strand at 2656.433 kb on + strand at 2656.433 kb on + strand at 2656.471 kb on + strand at 2656.471 kb on + strand at 2656.508 kb on - strand at 2656.518 kb on + strand at 2656.702 kb on + strand, within fliD at 2656.786 kb on + strand, within fliD at 2656.790 kb on + strand, within fliD at 2656.791 kb on - strand, within fliD at 2656.888 kb on + strand, within fliD at 2656.889 kb on - strand, within fliD at 2657.005 kb on + strand, within fliD at 2657.006 kb on - strand, within fliD at 2657.006 kb on - strand, within fliD at 2657.031 kb on - strand, within fliD at 2657.218 kb on + strand, within fliD at 2657.330 kb on - strand, within fliD at 2657.345 kb on + strand, within fliD at 2657.345 kb on + strand, within fliD at 2657.346 kb on - strand, within fliD at 2657.347 kb on + strand, within fliD at 2657.384 kb on + strand, within fliD at 2657.384 kb on + strand, within fliD at 2657.394 kb on + strand, within fliD at 2657.394 kb on + strand, within fliD at 2657.394 kb on + strand, within fliD at 2657.405 kb on - strand, within fliD at 2657.461 kb on + strand, within fliD at 2657.515 kb on + strand, within fliD at 2657.668 kb on + strand, within fliD at 2657.677 kb on + strand, within fliD at 2657.807 kb on + strand at 2657.863 kb on + strand at 2657.863 kb on + strand at 2657.937 kb on + strand at 2657.937 kb on + strand at 2658.198 kb on + strand, within fliS at 2658.339 kb on + strand at 2658.362 kb on - strand at 2658.533 kb on - strand, within fliT at 2658.648 kb on - strand, within fliT at 2658.657 kb on + strand, within fliT at 2658.658 kb on - strand, within fliT at 2658.659 kb on + strand, within fliT at 2658.763 kb on + strand at 2658.880 kb on + strand at 2658.880 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-Sucrose remove 2,655,534 - LU632_RS13835 0.46 +0.2 2,655,534 - LU632_RS13835 0.46 -0.4 2,655,662 - LU632_RS13835 0.56 -0.3 2,655,662 - LU632_RS13835 0.56 -0.6 2,655,962 - LU632_RS13835 0.79 +0.3 2,655,967 - LU632_RS13835 0.80 -1.8 2,655,967 - LU632_RS13835 0.80 -0.0 2,656,015 - LU632_RS13835 0.83 -0.7 2,656,230 + -0.3 2,656,259 + -0.3 2,656,388 + -1.0 2,656,433 + -0.4 2,656,433 + -0.2 2,656,471 + +0.0 2,656,471 + -0.6 2,656,508 - +0.4 2,656,518 + -0.4 2,656,702 + fliD LU632_RS13840 0.14 -0.7 2,656,786 + fliD LU632_RS13840 0.20 -2.0 2,656,790 + fliD LU632_RS13840 0.20 -0.8 2,656,791 - fliD LU632_RS13840 0.20 +0.4 2,656,888 + fliD LU632_RS13840 0.27 -0.1 2,656,889 - fliD LU632_RS13840 0.27 +0.7 2,657,005 + fliD LU632_RS13840 0.35 +0.4 2,657,006 - fliD LU632_RS13840 0.35 -0.2 2,657,006 - fliD LU632_RS13840 0.35 -0.3 2,657,031 - fliD LU632_RS13840 0.37 -1.4 2,657,218 + fliD LU632_RS13840 0.50 -1.2 2,657,330 - fliD LU632_RS13840 0.58 -0.2 2,657,345 + fliD LU632_RS13840 0.59 +1.2 2,657,345 + fliD LU632_RS13840 0.59 -0.5 2,657,346 - fliD LU632_RS13840 0.59 -0.3 2,657,347 + fliD LU632_RS13840 0.59 -1.3 2,657,384 + fliD LU632_RS13840 0.62 -1.0 2,657,384 + fliD LU632_RS13840 0.62 -0.7 2,657,394 + fliD LU632_RS13840 0.63 -1.7 2,657,394 + fliD LU632_RS13840 0.63 -0.6 2,657,394 + fliD LU632_RS13840 0.63 -1.0 2,657,405 - fliD LU632_RS13840 0.63 -0.3 2,657,461 + fliD LU632_RS13840 0.67 +0.1 2,657,515 + fliD LU632_RS13840 0.71 +0.4 2,657,668 + fliD LU632_RS13840 0.82 +0.0 2,657,677 + fliD LU632_RS13840 0.83 +0.6 2,657,807 + +0.1 2,657,863 + +0.5 2,657,863 + +1.0 2,657,937 + +1.8 2,657,937 + +0.8 2,658,198 + fliS LU632_RS13845 0.65 -0.5 2,658,339 + -2.5 2,658,362 - -0.3 2,658,533 - fliT LU632_RS13850 0.52 -0.9 2,658,648 - fliT LU632_RS13850 0.83 -0.5 2,658,657 + fliT LU632_RS13850 0.85 -0.6 2,658,658 - fliT LU632_RS13850 0.86 -0.3 2,658,659 + fliT LU632_RS13850 0.86 +0.1 2,658,763 + +0.5 2,658,880 + +0.2 2,658,880 + -0.2
Or see this region's nucleotide sequence